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Type :thesis
Subject :SD Forestry
Main Author :Siti Zubaidah Lood
Title :In vitro morphogenic response from different explants of sweet basils, Ocimum basillicum L.
Place of Production :Tanjong Malim
Publisher :Fakulti Teknikal dan Vokasional
Year of Publication :2018
Corporate Name :Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris
The purpose of this study was to examine the ability of seed germination Ocimum basilicum L. to germinate through tissue culture, the rate of time difference in seed germination in traditional or modern technology. In addition, callus formation was also carried out in the study to see the rate of cell differentiation at the exploratory part. At the end of the study, it was also tested by acclimatization of the explant to green house. The complete regeneration of the Ocimum basilicum L. plant and callus production was successfully produced through the tissue culture technique. The part of leaves, stems and roots of 8-week aseptic seedlings were used in this experiment. All cultures are stored at a temperature of 25 ± 1ºC and illuminated with fluorescent light for 16 hours of light, 8 hours dark. Plant acclimatization is done on three different soil types, namely garden soil, coconut powder and vermiculation. After completion, all data was recorded and analyzed using ANNOVA. Using MS medium (Murashige and Skoog, 1962) root explants were found to be very responsive for 0.5 mg / l BAP and 1.0 mg / l NAA concentration to produce maximum callus weight, and The stem explant for a concentration of 0.5 mg / l BAP with 1.5 mg / l of NAA will produce double shoots. Growth of seedlings are most optimum when regenerated from stem explants are acclimated in a garden with the survival rate of 88 ± 0.8%. As the conclusion, this method of tissue culture is the best method in producing new generation of Ocimum basilicum L. and its features are preserved. The implications of this study suggest that this tissue culture method can help to regenerate this species from extinction and the benefits of its use can continue to apply.

References

Abobkar I. M. Saad & Ahmad M. Elshahed (2012), Recent Advences in Plant in vitro

Culture, Chapter 2 : Plant Tissue Culture Media (pp. 22-40), INTECH

 

Acheapong  E.  (1982).  Multiplication  and  Conservation  of  Cocoyam  (Xarthosoma sajiltifoljum) 

germplasm by application of tissue culture methods Ph.D Thesis. University of Burmingham, UK

 

Aladele S. E. & Kuta D. (2008). Environmental and genotypic effects on the rowth rate  of  in  

vitro  cassava  plantlet  (Manihot  esceulenta)  African  Journal  of Biotechnology Vol. 7 (4), pp. 

381-385.

 

Aloni, R. (1980).  Role of auxin and sucrose in the differentiation of sieve and tracheary elements 

in plant tissue cultures. Planta. 150: 255 263.

 

Arditti, J., & Ernst, R. (1993). Micropropagation of Orchids. John Wiley & Sons, Inc, Canada, 

pp:1-665

 

Bapat, V.A., Mhatre, M. and Rao, P.S. (1987). Propagation of Morus indica (mulberry) by 

encapsulated shoot buds. Plant Cell Rep. 6: 393-395.

 

Bhojawi, S.S., & Razdan, M.K. (1996). Plant Tissue Culture: Theory and Practise, a Revised Edition, 

Elsever, Amsterdam, pp:39-62

 

Bhojwani,  S.S.  &  Razdan,  M.K.  (1992).  Plant  tissue  culture:  Theory  and  practice.

Elsivier, Amsterdam, London, New York, Tokyo.

 

Biren N. S., Avinash K. S. (2010), Pharmacognostic Studies of The Lagenaria siceraria

(Molina) Standley, Gujerat, India

 

Butenko, R.G. (1968). Plant tissue culture and plant morphogenesis. Translated from Russian.Israel 

program for Scientific Translation, Jerusalem.

 

Capuano  C,  Piccioni  E,  Standardi  A  (1998).  Effect  of  different  treatments  on  the 

conversion of M.26 apple rootstock synthetic seeds obtained from encapsulated apical and axillary 

micropropagated buds. J. Hort. Sci. 73:299-305.

 

Carew,D.P., & Staba, E.J. (1965). Plant tissue culture; its fundamentals, application and 

relationship to medicinal plant studies. Lloydia. 28: 1-26.

 

Cassells,   A.C.(1991).   Problems   in   tissue   culture:   Culture   contamination.   In: 

Micropropagation   Technology   tind   Application.   P.C.   Debergh   and   R.H. Zimmerm, cds. 

Kluwer Academic Publishers. Dordrecht, Netherlands. pp. 31-44.

 

Chaleff, R. S., and P. S. Carlson. (1974). Somatic cell genetics of higher plants. Ann.

Rev. Genet. 8: 267-278.

 

Chan  , P.Y., Fujita, S., Kohno, K., Ashrafuzzaman, M.D., Nakamura, N. and Hayashi,

N. (2000). Purification and characterization of polyphenol oxidase from banana (Musa sapietum L.) pulp. Journal of Agricultural and Food Chemistry 48: 2732-

2735.

 

Cocking, E. C. 1972. Plant cell protoplasts-isolation and development. Ann. Rev. Plant Physiol. 23: 

29-50.

 

Conger, B. V. (1980). Cloning Agricultural Plants via In-vitro Techniques, CRC Press Inc, Boca 

Raton, Florida, pp; 1-50

 

Dalmacio I. F. (1992). Biotechnology for sustainable Agriculture National Institute of 

Biotechnology and Applied Microbiology, University of the Philippines.Dalziel,

J. M. 1937. The useful plants of West Tropical Africa. Whitefriars Press, London, 612 pp.

 

Daniel, R.L. (1998). The many dimension of plant tissue culture research. Webmaster of Aggie 

Horticulture Publications, pp.201-210.

 

de Fossard R.(1976)  Tissue culture for plant propagation. Armidale: University of New England.

 

Driver,  J.A.,  &  Kuniyuki,  A.H.  (1984)  In  vitro  propagation  of  paradox  walnut rootstock. 

Hotr. Sci., 19: 507-709.

 

Encyclopedia of Life (2009), Introduction.  http://eol.org/pages/281/overview 

 

Enjalric,  F.,  Carron,  M.P.,  Lardet,  L.(1988).  Contamination  of  Primary  Cultures  in

Tropical Areas: The Case of Hevea Brasiliensis. In:Bacterial and Bacteria-like Contaminants of 

Plant Tissue Cultures. ISHS Acta Horticulturae. 225:

 

Fabijan D, Taylor JS & Reid DM (1981) Adventitious rooting in hypocotyls of sunflower (Helianthus 

annuus) seedlings II. Action of gibberellins, cytokinins, auxins and ethylene. Physiologia 

Plantarum 53: 589?597.

 

Food  and  Agriculture  Organization  (FAO).  (2005).  Statistics  Database  (Agriculture Data) 

2001. On internet http:/apps.fao.org.

 

Frusciante, L.,  Barone, A.,Carputo,D. Ercolano, M.R., Della Rocca, F., & Esposito,

S. (2000). Evaluation and use of Plant Biodiversity for Food and Pharmaceutical. Fitoterpia. 71 : 

66 – S72.

 

Gamborg, O., Miller, R. & Ojima, K.  1968). Nutrient requirement suspensions cultures of soybean 

root cells. Experimental Cell Research. 50(1):151-158.

 

Gautheret, R. J. (1934). Culture du tissus cambial. Comptes Rendus Hebdomadaires des Séances de 

l'Académie des Sciences, 198, 2195–2196.

 

Gautheret, R. J. (1935). Recherches sur la culture des tissus végétaux. Ph.D. Thesis,

Paris

 

Gautheret, R. J. (1939). Sur la possibilité de réaliser la culture indéfinie des tissus de 

tubercules   de   carotte. Comptes   Rendus   Hebdomadaires   des   Séances   de l'Académie des 

Sciences, 208, 118–120.

 

Gbile  Z.O.(1998).  Collection,  Conservation  and  Utilization  of  medicinal  plants.  In Robert 

PA, & Janice EA (eds.). Conservation of Plant Genes III: Conservation and Utilization of African 

Plants. Missouri Botanical Gardens Press. pp. 163-17

 

Georges, D., Chenieux, J.C. & Ochatt. S.J. (1993). Plant regeneration from aged-callus of the woody 

ornamental species Lonicera japonica cv. "Hall's prolific". Plant Cell Reports. 13:91-94. Growth of 

monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199-204.

 

Han,  J-S.,  Oh,  D-G.,  Mok,  I-G.,  Park,  H-G.,  Kim,  C.  K.,  (2004),  Efficient  plant 

regeneration  from  cotyledon  explants  of  bottle  gourd  (Lagenaria  siceraria Standl.). Plant 

Cell Rep., 23:291-296 Improvement of cucurbits; In: Biology and utilization of the cucurbitaceae; 

pp. 367?381 eds. D M Bates, R W Robinson & C Jeffrey(Ithaca, London: Cornell Univ. Press).

 

Hartmann, H.T., Kester, D.E. & Davies, F.T. (1990). Plant propagation: principles and practices. 

Englewood Cliffs, NJ: Prentice Hall.

 

IUCN (2006). IUCN Red List of Threatened Species. IUCN, Gland, Switzerland 

 

Kanchanapoom,  K.,  Jantaro,  S.  &  Rakchad,  D.  (2001).  Isolation  and  fusion  of

protoplasts from mesophyll cells of Dendrobium pompadour. Science Asia. 27:

29-34.

 

Kane,     M.     (2003).     Bacterial     and     fungal     indexing     of     tissue     

cultures. http://www.hos.ufl.edu/

 

Nwe, N., T., & Tamur, H. (2010). Production of Fungal Chitosan by Enzymatic Method and Applications 

in Plant Tissue Culture and Tissue Engineering: 11 Years of Our    Progress,    Present    

Situation    and    Future    Prospects. Biopolymers. doi:10.5772/10261

 

Kasha, K. J. (1974). Haploids in Higher Plants. University of Guelph, Guelph.

 

Katare, C., Agrawal, S., Jain, M., Rani, S., Saxena, S., Bisen, P.S., Prasad, G.B.K.S. (2011). 

Lagenaria siceraria : A Potential Source of Anti-Hyperlipidermic and Other Pharmacological Agents. 

Current Nutrition & Food Science Vol. 7, No 3, pp 1- 9

 

Krikorian  AD  (1995)  Hormones  in  tissue  culture  and  micropropagation.  In:  Plant hormones:  

          physiology, biochemistry and molecular biology (P.J. Davies, ed.). Kluwer Academic        

  Publishers, Dordrecht, p. 774?796.

 

Lee, J.M. (1994). Cultivation of grafted vegetables I. Current status, grafting methods, and 

benefits. HortSci. 29: 235-239.

 

Lee, J.M. (2003). Advances in vegetable grafting. Chronica Horticulturae. 43:13-19.

 

Lloyd,   G.   &   McCown,   B.   (1980)   Commercially-feasible   micropropagation   of 

mountainlaurel, Kalmia latifolia, by use of shoot-tip culture. Intern. Plant Prop. Soc. Proc. 30: 

421–427.

 

Mendi, Y.Y., Ipek, M., Buzkan, N., Kacar, Y. A., Curuk, S., (2009), Regeneration and Histological 

Analysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.). Turk J Agric For 

33(2009) pp. 165-172

 

Moreno V & Roig LA (1990) Somaclonal variation in cucurbits; In: Biotechnology in agriculture and 

forestry Vol 11: Somaclonal variation in crop improvement I; pp 435?464 ed. Y P S Bajaj (Berlin, 

Heidelberg:Springer? Verlag).

 

Murashige T, Skoog F.  (1962) A revised medium for rapid growth and bioassays with tobacco tissue 

cultures. Physiol. Plant.; 15: 473-479.

 

Murashige,  T.  (1974).  Plant  propagation  through  tissue  cultures.  Ann.  Rev.  Plant Physiol. 

25: 135-166.

 

Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco 

cultures, Physiol. Plant. 15:473-497

 

Murashige, T.; Skoog, F. (1962), A revised medium for rapid growth and bioassays with tobacco 

tissue cultures. Physiol. Plant, 15, 473-497.

 

Nakamura, K., Wakita, Y. & Yokota, S. (1995). Induction of multiple shoots by shoot apex culture in 

Magnolia obovata Thunb. Plant Tissue Culture Letters. 12: 34- 40.

 

Nishibayashi,  S.,  H.  Kaneko  &  T.  Hayakawa.  (1996).  Transformation  of  cucumber (Cucumis 

sativus L.) plants using Agrobacterium tumefaciens and regeneration from hypocotyl explants. Plant 

Cell Rep. 15: 809-814.

 

Nitsch JP 1951. Growth and development in vitro of excised ovaries. Am. J. Bot. 38: 566?577.

 

Nitsch, J.P. & Nitsch, C (1969) Haploid plants from pollen grains. Science. 163: 85–87

 

Nobécourt, P. (1939). Sur la pérennité  et l’augmentation de volume des cultures de tissues 

végétaux. Comptes Rendus des Séances de la Société de Biologie et de ses Filiales, 130, 1270–1271.

 

Okere, A. U. & Adegeye, A. (2011). In vitro propagation of an endangered medicinal timber species 

Khaya grandifoliola C. Dc. African Journal of Biotechnology Vol. 10(17), pp. 3335-3339

 

Philips, Greogory, C. (2004). In vitro morphogenesis in plants – recent advances. In Vitro Cellular 

and Development Biology – Plant. 40: 342-345

 

Pierik, R. I. M. (1987). In vitro Culture of Higher Plants. Martinus Nijhoff Publishers,

Dordrecht, ppt:1-344

 

Puhan, Z., & Martin. S. M. (1971). The industrial potential of plant cell culture. Prog.

Ind. Microbiol. 9: 13-39.

 

Reddy M. C., Murthy K. S. R & Pullalah T (2012), Synthetic seeds : A review in agriculture and 

forestry, African Journal of Biotechnology Vol. 11(78), pp. 14254-14275

 

S. Habibur Rahman (2003), Bottle Gourd (Lagenaria siceraria)  A vegetable good for health, Research 

and Development, Amal Herbal Formulation Pvt. Ltd., M. G. Road, Ghatkopar, Mumbai

 

Saidin, I. Sayuran tradisional, ulam dan penyedap rasa (2000). Penerbit Universiti kebangsaan 

Malaya. pp 1-33

 

Sarowar S, Oh HY, Hyung NI, Min BW, Harn CH,Yang SK, Ok SH & Shin JS (2003) In  vitro  

micropropagation  of  a  Cucurbita  interspecific  hybrid  cultivar  ?  a  root stock plant. Plant 

Cell Tiss. Organ Cult. 75: 179?182.

 

Sarowar, S., H.Y. Oh, N.I. Hyung, B.W. Min, C.H. Harn, S.K. Yang, S.H.Ok & J.S. Shin. (2003). In 

vitro propagation of a Cucurbita interspecific hybrid cultivar-a root stock plant. Plant Cell, 

Tissue Org Cult. 75: 179-182.

 

Schenk, R. V., & A. C. Hildebraudt. 1972. Medium and techniques for induction and Skoog, F., & 

Miller, C.O.(1957). Chemical regulation of growth and organ formation

in plant tissue cultured in vitro . Symp. Exp Biol. 11:118-131.

 

Skoog, F., Miller, C.O (1957). Chemical regulation of growth and organ formation in plant tissue 

cultured in-vitro. Symp. Exp Biol. 11:118-131

 

Steiner AA, Winden H. (1970) Recipe for ferric salts of ethelenediaminetetracetic acid. Plant 

Physiol.; 46: 862- 863.

 

Steward, F.C., Mapes, M.O., & Mears, K. (1958). Growth and organized development of cultured cells. 

II. Organization in cultures grown from freely suspended cells, American Journal of Botany. 

45:705-708

 

Street,  H.E.  (1973).  Plant  tissue  and  cell  culture.  Botanical  Monogr.  Blackwell 

Scientific Publications, London. 2.

 

Taji, A., Prakash, P.K. & Prakash, L. (2002). In Vitro Plant Breeding. Howarth Press Inc. New York

 

Tempe,  J.,  Ed.  (1972).  Protoplastes  et  Fusion  de  Cellules  Somatiques  Vegetales.

Colloq. Int. Cent. Natl. Rech. Sci., Paris. 222.

 

Thomas,   D.T   &   Philip   B.   (2005).   Thidiazuron-induced   high   frequency   shoot 

organogenesis from leaf-derived callus of a medicinal climber,Tylophora indica

(Burm.F)  Merrill.In Vitro Cell .Dev.Biol- Plant. 41:124-128.

 

Thomas,  T.D.  &  Jacob,  A.,  2004.  Direct  somatic  embryogenesis  of  Curculigo orchioides 

Gaertn. an Endangered medicinal herb. J. Plant Biotech., 6 :193-197.

 

Thorpe,  T.  A.  (1990).  The  current  status  of  plant  tissue  culture.  In  S.  S.  Bhojwani 

(Ed.), Plant tissue culture: Applications and limitations (pp. 1–33). Amsterdam: Elsevier.

 

Torres KC. (1989), editor. Tissue culture techniques for horticultural crops. New York, London: 

Chapman and Hall.

 

Torres, K.C. (1989). Tissue culture techniques for horticultural crops. AVI Books, New.

York.

 

Vasil, I. K., & V. Vasil. (1972) Totipotency and embryogenesis in plant cell and tissue cultures. 

In Vitro. 8: 117-125.

 

Vinterhalter D, Vinterhalter BS. Micropropagation of Dracaena sp. (1997)In: Bajaj YPS (ed.) 

Biotechnology in agriculture and forestry 40, High-tech. and Micropropagation VI. Berlin, 

Heidelberg: Springer; . p131- 146.

 

Wehner TC, Cade RM & Locy RD (1990) Cell, tissue and organ culture techniques for genetic

 

White, P. R. 1963. The Cultivation of Animal and Plant Cells. 2nd ed. Ronald Press, New York.

 

White,  P.R.  (1943).  A  Handbook  of  Plant  Tissue  Culture.  Jacques  Cattell  Press,

Lancaster, PA. pp:227

 

 

 

 

 


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