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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| The genus Premna in Lamiaceae family was categorized into two groups namely P. serratifolia-group and P. trichostoma-group by de Kok in 2013. The division was based on three distinct morphological characters which are twigs, calyx and fruit. This group category lacks of anatomical and micromorphological evidence and solely based on those morphological characters. Therefore, in this study, anatomical and micromorphological characters were analysed on six Premna species (P. interrupta, P. serratifolia, P. trichostoma, P. tomentosa, P. parasitica and P. odorata) from few localities in Peninsular Malaysia. They were investigated to determine the size of stomata and some epidermal characters among the species members to provide some evidence towards the division. The methods involved in this research were epidermal peel preparation and field emission microscopy (FESEM) technique. The stomata were observed under the microscope and also photographed at different magnifications. Based on the observation, primary sculpturing pattern of leaf surface showed four types of patterns and labelled as type 1, 2, 3 and 4. All six species of Premna have the same undulating, four sides and number of anticlinal walls which showed shared characteristics of the genus. However, the stomatal size, sculpturing and distribution show some taxonomic significance in identification of the species. Stomatal size shows significant difference between species which ranged from 3 m to 20 m. Three types of stomata presented at the abaxial surface, anisocytic, paracytic and diacytic while four different types of stomatal sculpturing were observed which are type 1, 2, 3, 4 and stomatal distribution resulted in variances in stomatal density. Therefore, the division of Premna as suggested by de Kok was supported based on stomatal type and secondary stomata sculpturing evidences. Malaysian Journal of Microscopy (2023). All rights reserved. |
| References |
Harley, R. M., Atkins, S., Budantsev, P. D., Cantino, P. D., Conn, B. J.,Grayer,R.,Harley, M. M., De Kok, R., Kresstovskaja, T., Morales, R., Paton, A. J.,Ryding, O.& Upson, T. (2004). Labiatae.In TheFamilies and Genera of Vascular Plants, vol. 7.Ed. Kubitzki, K. Flowering Plants-Dicotyledons.(Springer-Verlag,Germany),pp167–275. Mabberley, D. J. (2008). Architecture of Verbenaceae of Madang Province, PapuaNew Guinea, with preliminary notes on their reproductive biology. Science New Guinea. 19(1), 37-45. Verdcourt, B. (1992). Verbenaceae.InFlora of Tropical EastAfrica. Ed.Polhill, R. M. (Balkema, Rotterdam), 623-624. Corner, E. J. H. (1940). Notes on the systematic and distribution of Malayan phanerogams III, In two volumes: Wayside Tree of Malaya, vol.10. Cherry, W. T. (Government Printing Office, Singapore), pp. 239-260. de Kok, R. P. J. (2013). The genus PremnaL. (Lamiaceae) in the FloraMalesiana area. Kew Buletin.68(1), 1-30. de Kok, R. P. J. (2013). The genus PremnaL. (Lamiaceae) in the Flora Malesianaarea. Kew Bulletin.68(1), 55-84. Holland, N. & Richardson A. (2009). Stomatal Length Correlates with Elevation of Growth in Four Temperate Species. Journal of Sustainable Forestry.28(1), 63-73. Burkill, I. H. (1935). A Dictionary of the economic products of the Malay Peninsula.Vol. II.3rd printing (Governments of Malaysia and Singapore by the Ministry of Agriculture and Co-operatives, Kuala Lumpur, Malaysia.) pp. 2277-2282. Klaudija, C., Marko, P., Martina, G., Jasna, P., Dalibor, B., Mirjana, H. C. & Zlatko, S. (2016). Medicinal Plants of the Family Lamiaceae as Functional Foods -a Review. Czech Journal of Food Science. 34(5), 377-390. Yi, L. T., Li, J., Geng, D., Liu, B. B., Fu, Y., Tu, J. Q.,Liu, Y. & Weng, L. J. (2013). Essential oil of Perilla frutescens-induced change in hippocampal expression ofbrain-derived neurotrophic factor in chronic unpredictable mild stress in mice. Journal of Ethnopharmacology.147(1), 245-253. Atul, K., Ruchika. K. &Uttam. S. B. (2015). PremnaSpecies: A Review. Journal of Biological andChemicalChronicle.1(1), 55-59. Li, B., Cantino, P. D., Olmstead, R. G., Bramley, G. L., Xiang, C. L., Ma, Z. H., Tan, Y. H. & Zhang, D. X.(2016).A large-scale chloroplast phylogeny of the Lamiaceae sheds new light on its subfamilialclassification.Scientific Reports. 6(34343), 1-18. Zhao, F., Chen, Y. P., Salmaki, Y., Drew, B. T., Wilson, T. C., Scheen, A. C., Celep, F., Bräuchler, C., Bendiksby, M., Wang, Q., Min, D. Z., Peng, H., Olmstead, R. G., Li, B. & Xiang, C. L.(2021). An updated tribal classification of Lamiaceae based on plastome phylogenomics. BMC Biology. 19(2), 1-27. Bramley, G.L.C. (2019). Flora Malesiana.Series I: Volume 23. Lamiaceae. (National Park Board Singapore)pp. 324-326. Noraini, T., Che Nurul Aini, C. A., Nurhanim, M. N., Nabilah, M., Mohd Norfaizal, G., Ahmad Fitri, Z. &Hamidun, B. (2022). Taxonomic Significance of Leaf Epidermis Micromorphological Characteristics of PentaceL. (Malvaceae s.l) in Malaysia. Malaysian Journal of Microscopy. 18(1), 192-201. Syanawiyyah, M. Z., Fatimah, M. & Nor Nafizah, M. N. (2022). Numerical Taxonomic Evaluation of Leaf Architectural Morphology of VitexL. species (Lamiaceae Martinov) in Peninsular Malaysia. Journal of Science and Mathematics Letters.10(2), 1-15. Mazatul Azrin, R., Nor Nafizah, M. N., Fatimah, M. & Norhayati, D.(2019). Leaf morphology and numerical taxonomic evaluation of ClerodendrumL. (Lamiaceae).The Malayan Nature Journal.71(1), 97-109. Nor Nafizah, M. N., Fatimah, M., Hasimah, A., Mazatul Azrin, R. & Hilda, E. (2018).Comparative anatomical study and morphological observation of three medicinal Vitex species (Lamiaceae). The Malayan Nature Journal. 70(3), 285-296. Ramayya, N. & Rao, V. J. (1969). Range of structural and ontogeneticstomatal variations in three species of Ocimum(Labiatae). Engineering and Technology Journals.38(4), 79-82. Metcalfe C. R. & Chalk L. (1950). Anatomy of dicotyledons. Vol. II. (Clarendon Press, Oxford.) pp. 243-245. Inamdar, J. A. & Bhatt, D. C. (1972). Structure and development of stomata in some Labiatae. Annals of Botany. 36(2), 335-344. El-gazzar, A. & Watson, L. (1970). A taxonomic study of Labiatae and related genera. TheNew Phytologist.69(2), 451-486. De Kok, R. P. J., Grayer, R. J. & Kite, G. C. (2000). Relationships of the endemic Australian genus Huxleya Ewart & Rees(Labiatae) based on fruit andflavonoidcharacters.AustralianSystematicBotany.13(3), 425-428. |
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