The effect of Yttria-Stabilized Zirconia (YSZ) addition on the synthesis of? ETA-Tricalcium Phosphate (?-TCP) from biogenic hydroxyapatite

Ismail Zainol

Abstract

The utilization of ?-tricalcium phosphate (?-TCP) in medical field has been on demand for their excellent biocompatibility, bioactivity and solubility properties. This material can be produced through degradation of hydroxyapatite (HA) at high temperature. In this research, biogenic hydroxyapatite from fish scales (FsHA) was selected as pre-cursor for the synthesis of ?-TCP. The effect of yttria-stabilized zirconia (YSZ) addition on degradation of FsHA into ?-TCP have been investigated. Different amount of YSZ ranging from 5 to 15 wt% were mixed with FsHA and ball milled into fine powder. The mixture was then sintered at temperature of 1200 C. The materials were characterized using X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). The results from FTIR, XRD and SEM-EDX analyses proved that the presence of ?-TCP in the sintered sample at 1200oC. The percentage of ?-TCP was calculated ranging from 17 to 21 % as analyzed from XRD pattern. It was found that the FsHA/YSZ composite with 5 wt% of YSZ addition produced the highest composition of ?-TCP with smallest crystallite size at the ideal sintering temperature of 1200 C. The results also shown that the FsHA was not fully converted into ?-TCP during sintering. Furthermore, XRD analysis also clearly shown the presence of another phase known as calcium zirconate (CaZrO3) phase. The triphasic calcium phosphate (BCP) has high potential to be used as biomaterials for bone fillers applications. Malaysian Journal of Microscopy (2023). All rights reserved.

References

Suresh, S., Romana, S., Md-Zillur, R., Muhamad, F. I., Anita, L., Is, F., Noor, H. M. K.&Won-Chun, O. (2023). Recent advancements in polymer matrix nanocomposites for bone tissue engineering applications. Journal of Drug Delivery Science and Technology.82, 104313.

Zainol, I., Zainurin, M.A.N., Bakar, N.H.A., Jaafar, C.N.A. &Mudhafar, M. (2022). Characterisation of porous hydroxyapatite beads prepared from fish scale for potential bone filler applications.Malaysian Journal of Microscopy. 18 (2), 48-57

Mariana F. V., Nunes, P. J., Sónia, P. M., Ilídio, J. C.&Abílio, P.S. (2019). Microstructural, mechanical and biological properties of hydroxyapatite-CaZrO3biocomposites. Ceramics International. 45, 8195–8203.

Joviniano, M. O. J., Pedro, G.M., Rafael, C. C. & Elizabeth, F. M. (2021). Physical characterization of biphasic bioceramic materials with diferent granulation sizes and their infuence on bone repair and infammation in rat calvaria. Scientifc Reports. 11, 4484.

Atchara, K., Thanachai, B., Wantana, K., Penphitcha, A., H-thaichnok, C., Nutth, T., Adulphan, P., Sujittra, D., Pitphichaya, S., Wutthigrai, S., Drusawin, V.,Atipong, B.&Boonrat, L. (2021). Sintering behavior and mechanical properties of hydroxyapatiteceramics prepared from Nile Tilapia (Oreochromis niloticus) bone and commercial powder for biomedical applications. Ceramics International. 47, 34575–34584.

Evis, Z., Ergun, C., & Doremus, R.H. (2005). Hydroxylapatite–zirconia composites: thermal stability of phases and sinterability as related to the CaO–ZrO2 phase diagram. Journal of Materials Science. 40(5), 1127–1134.

Ferreira, C.R.D., Santiago, A.A.G., Vasconcelos, R.C., Paiva, D. F.F., Pirih, F.Q., Araújo, A. A., Motta, F.V.&Bomio, M.R.D. (2022). Study of microstructural, mechanical, and biomedical properties of zirconia/hydroxyapatite ceramic composites. Ceramics International.48, 12376–12386.

Evis, Z. (2006). Reactions in hydroxylapatite-zirconia composites. CeramicsInternational. 33(6), 987–91.

Evis, Z., Usta, M. & Kutbay, I. (2008). Hydroxyapatite and zirconia composites: effect of MgO and MgF2 on the stability of phases and sinterability. Materials Chemistry and Physics. 110(1), 68–75.

Ananth, K.P., Shanmugam, S., Jose, S.P., Nathanael, A.J., Oh, T.H., Mangalaraj, D. & Ballamurugan, A.M. (2015). Structural and chemical analysis of silica-doped β-TCP ceramic coatings on surgical grade 316L SS for possible biomedical application. Journal of Asian Ceramic Societies. 3, 317-324.

Joint Committee for Powder Diffraction Studies [JCPDS] –International Center for Diffraction Data, and American Society for Testing and Materials, Powder Diffraction File. Joint Committee for Powder Diffraction Studies, Swarthmore, PA, 1991.

Stoch, P., Szczerba, J., Lis, J., Madej,D.&Pedzich, Z. (2012).Crystal structure and ab initio calculations of CaZrO3, Journal of the European Ceramic Society. 32, 665–670.

Rapacz-Kmita, A., Slosarczyk, A., Paszkiewicz, Z. & Paluszkiewicz, C. (2004). Phase stability of hydroxyapatite-zirconia (HAp/ZrO2) composites for bone replacement. Journal of Molecular Structure. 704(1-3), 333-340.

Towler, M. R. & Gibson, I. R. (2001). The effect of low levels of zirconia addition on the mechanical properties of hydroxyapatite. Journal of Materials Science Letters. 20, 1719–1722.

Balaji, D.S., Basavaraja, S., Deshpande, R., Mahesh, D.B., Prabhakar, B.K. & Venkataraman, A. (2009). Extracellular biosynthesis of functionalized silver nanoparticles by strains of Cladosporium cladosporioides fungus. Colloids and Surfaces B: Biointerfaces. 68, 88-92.

Ruys, A. (2019). Processing, structure, and properties of alumina ceramics. In Alumina Ceramics: Biomedical and Clinical Applications.(Woodhead Publishing Series in Biomaterials),pp. 71–121.

Parashurama, L. & Devia, V.S.A. (2018). Combustion Synthesis of Calcium Zirconate Ceramic Nano Powders -Their Structural and Morphological Studies. Der Pharmacia Lettre.10(7), 65-69.

Ashok, M., Sundaram, N.M.& Kalkura, S.N. (2003). Crystallization of hydroxyapatite at physiological temperature. Materials Letters. 57, 2066–2070.

Bollino, F., Armenia, E. & Tranquillo, E. (2017). Zirconia/Hydroxyapatite Composites Synthesized Via Sol-Gel: Influence of Hydroxyapatite Content and Heating on Their Biological Properties.Materials.10, 757.

Mansour, S.F., El-dek, S.I. & Ahmed, M.K. (2017). Physico-mechanical and morphological features of zirconia substituted hydroxyapatite nano crystals. Science Report. 7,4320.

Sayer, M., Stratilatov, A.D., Reid, J., Calderin, L., Stott, M.J., Yin, X., Mackenzie,M., Smith, T.J.N., Hendry, J.A. & Langstaff, S.D. (2003). Structure and composition of silicon-stabilized tricalcium phosphate. Biomaterials. 24, 369–382.

Salehi, S. & Fathi, M.H. (2010). Fabrication and characterization of sol–gel derived hydroxyapatite/zirconia composite nanopowderswith various yttria contents.Ceramics International. 36, 1659–1667.