UPSI Digital Repository (UDRep)
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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| Hydroxyapatite (HA) coatings on metallic implants have been extensively used in orthopedic applications to improve tissue-implant interactions, enhance their biocompatibility, and enhance their functionality. However, the expensive synthetic HA is the most widely used bioceramic for implant coatings, leading to high implants costs. Hence, this research explored the potential of an inexpensive biogenic HA derived from fish scales and FsHA/yttria-stabilized zirconia (YSZ) bioceramic coatings on a Ti6Al4V alloy as an alternative to synthetic HA coatings. The FsHA/YSZ powders and the coatings were examined with X-ray diffraction (XRD) and scanning electron microscopy/energy dispersive X-ray (SEM/EDX), and the surface roughness, microhardness, corrosion resistance, bioactivity, and in vitro cytotoxicity of the coatings were also determined. The morphological powder analysis revealed particles with a slightly irregular morphology and a fine spherical morphology, while the coating microstructure analysis revealed a fine lamellar morphology, with partially melted and unmelted FsHA particles, and fine microcracks along with evenly dispersed ZrO2 particles. The surface roughness of the FsHA coating increased by 87.5% compared with the uncoated substrate, and the addition of YSZ significantly reduced this value. A 35.5% increase in hardness was obtained in the FsHA + 20 wt.% YSZ coating, and the FsHA coating showed a 43.2% reduction in the corrosion rate compared with the uncoated substrate; a further 73% reduction was observed with the addition of YSZ. The microstructure of the coatings after 14 days of immersion in simulated body fluid (SBF) revealed enlarged cracks and delaminated segments with well-grown apatite spherulite layers on the whole surface of the coatings, while in vitro cytotoxicity analysis showed a good cell viability of 95% at the highest concentration of the specimen. 2023 by the authors. |
| References |
Rey, C.; Combes, C.; Glimcher, M.J. Bone mineral: Update on chemical composition and structure. Osteoporos. Int. 2009, 20, 1013–1021. [CrossRef] [PubMed] Eichert, D.; Drouet, C.; Sfihi, H.; Rey, C.; Combes, C. Nanocrystalline apatite-based biomaterials: Synthesis processing and characterization. In Biomaterials Research Advances; Kendall, J.B., Ed.; Nova Science Publishers: New York, NY, USA, 2008. Xue, W.; Moore, J.L.; Hosick, H.L.; Bose, S.; Bandyopadhyay, A.; Lu, W.W.; Cheung, K.M.C.; Luk, K.D.K. Osteoprecursor cell response to strontium-containing hydroxyapatite ceramics. J. Biomed. Mater. Res. A 2006, 79, 804–814. [CrossRef] [PubMed] Suchanek,W.; Yoshimura, M. Processing, Properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants. J. Mater. Res. 1998, 13, 94–117. [CrossRef] de Bruijn, J.D.; van Blitterswilk, C.A.; Davis, J.E. Initial bone matrix formation at the hydroxyapatite interface in vivo. J. Biomed. Mater. Res. 1995, 29, 89–99. [CrossRef] Grootde, K.; Wolke, J.G.C.; Jansen, J.A. Calcium phosphate coatings or medical Implants. Proc. Inst. Mech. Eng. H 1998, 212, 137–147. [CrossRef] Hing, K.A.; Revell, P.A.; Smith, N.; Buckland, T. Effect of silicon level on rate, quality and progression of bone healing within silicate substituted porous hydroxyapatite scaffolds. Biomaterials 2006, 27, 5014–5026. [CrossRef] Morks, M.F. Fabrication and characterization of plasma-sprayed HA/SiO2 coatings for biomedical application. J. Mech. Behav. Biomed. Mater. 2008, 1, 105–111. [CrossRef] Gu, Y.W.; Khor, K.A.; Pan, D.; Cheang, P. Activity of plasma sprayed yttria stabilized zirconia reinforced hydroxyapatite/Ti–6Al–4V composite coatings in simulated body fluid. Biomaterials 2004, 25, 3177–3185. [CrossRef] Wu, C.; Ramaswamy, Y.; Chang, J.; Woods, J.; Chen, Y.; Zreiqat, H. The effect of Zn contents on phase composition, chemical stability and cellular bioactivity in Zn-Ca-Si system ceramics. J. Biomed. Mater. Res. Part B Appl. Biomater. 2008, 87, 346–353. [CrossRef] Xie, Y.; Liu, X.; Zheng, X.; Ding, C.; Chu, P.K. Improved stability of plasma-sprayed dicalcium silicate/zirconia composite coating. Thin Solid Film. 2006, 515, 1214–1218. [CrossRef] Piconi, C.; Maccauro, G. Zirconia as a ceramic biomaterial. Biomaterials 1999, 20, 1–25. [CrossRef] [PubMed] Kohorst, P.; Borchers, L.; Strempel, J.; Stiesch, M.; Hassel, T.; Bach, F.W.; Hübsch, C. Low-temperature degradation of different zirconia ceramics for dental applications. Acta Biomater. 2012, 8, 1213–1220. [CrossRef] [PubMed] Chou, B.Y.; Chang, E.; Yao, S.Y.; Chen, J.M. Phase transformation during plasma spraying of hydroxyapatite–10-wt.%-zirconia composite coating. J. Am. Ceram. Soc. 2002, 85, 661–669. [CrossRef] Chou, B.Y.; Chang, E. Plasma-sprayed hydroxyapatite coating on titanium alloy with ZrO2 second phase and ZrO2 intermediate layer. Surf. Coat. Technol. 2002, 153, 84–92. [CrossRef] Fu, L.; Khor, K.A.; Lim, J.P. Effects of yttria stabilized zirconia on plasma sprayed hydroxyapatite/yttria stabilized zirconia composite coatings. J. Am. Ceram. Soc. 2002, 85, 800–806. [CrossRef] Kasugu, T.; Yoshida, M.; Ikushima, A.J.; Tuchiya,M.; Kusakari, H. Stability of zirconia-toughened bioactive glass-ceramics—In vivo study using dogs. J. Mater. Sci. Mater. Med. 1993, 4, 36–49. [CrossRef] Duong, H.M.; Myint, S.M.; Tran, T.Q.; Le, D.K. Post-Spinning Treatments to Carbon Nanotube Fibers; Elsevier Ltd.: Amsterdam, The Netherlands, 2020. Tebyanian, H.; Norahan, M.H.; Eyni, H.; Movahedin, M.; Mortazavi, S.M.J.; Karami, A.; Nourani, M.R.; Baheiraei, N. Effects of collagen/ -tricalcium phosphate bone graft to regenerate bone in critically sized rabbit calvarial defects. J. Appl. Biomater. Funct. Mater. 2019, 17, 2280800018820490. [CrossRef] Deram, V.; Minichiello, C.; Vannier, R.N.; le Maguer, A.; Pawlowski, L.; Murano, D. Microstructural characterizations of plasma sprayed hydroxyapatite coatings. Surf. Coat. Technol. 2003, 166, 153–159. [CrossRef] Lawrence, S.K.; Gertrude, S.M. Studies on the relationship of the chemical constituents of blood and cerebrospinal fluid. J. Exp. Med. 1925, 42, 565–591. Cachinho, S.C.P.; Correia, R.N. Titanium scaffolds for osteointegration: Mechanical, in vitro and corrosion behavior. J. Mater. Sci. Mater. Med. 2008, 19, 451–457. [CrossRef] Yang, Y.; Kim, K.H.; Ong, J.L. A review on calcium phosphate coatings produced using a sputtering process: An alternative to plasma spraying. Biomaterials 2005, 26, 327–337. [CrossRef] [PubMed] Fernandez, J.; Gaona, M.; Guilemany, J.M. Tribological study of plasma hydroxyapatite coatings. Key Eng. Mater. 2004, 16, 383–386. [CrossRef] Habibovic, P.; Barrère, F.; de Groot, K. Learning from Nature How to Design New Implantable Biomaterials: From Biomineralization Fundamentals to Biomimetic Materials and Processing Routes. NATO Sci. Ser. 2005, 171, 105–121. Akram, M.; Ahmed, R.; Shakir, I.; Ibrahim, W.A.W.; Hussain, R. Extracting hydroxyapatite and its precursors from natural resources. J. Mater. Sci. 2014, 49, 1461–1475. [CrossRef] Milovac, D.; Gamboa-martínez, T.C.; Ivankovic, M.; Gallego, G.; Ivankovic, H. PCL coated hydroxyapatite scaffold derived from cuttle fish bone: In vitro cell culture studies. Mater. Sci. Eng. C 2014, 42, 264–272. [CrossRef] Mohd Pu’ad, N.A.S.; Koshy, P.; Abdullah, H.Z.; Idris, M.I.; Lee, T.C. Syntheses of hydroxyapatite from natural sources. Heliyon 2019, 5, E01588. [CrossRef] Ratner, B.D.; Hoffman, A.S.; Schoen, F.J.; Lemons, J.E. Biomaterials Science: An Introduction to Materials in Medicine, 2nd ed.; Elsevier: Toronto, ON, Canada, 2004. Doostmohammadi, A.; Monshi, A.; Fathi, M.H.; Braissant, O.A. A comparative physicochemical study of bioactive glass and bone-derived hydroxyapatite. Ceram. Int. 2011, 37, 1601–1607. [CrossRef] Sunil, B.R.; Jagannatham, M. Producing hydroxyapatite from fish bones by heat treatment. Mater. Lett. 2016, 185, 411–414. [CrossRef] Andronescu, E.; Grumezescu, A.M.; Madalina-Ionela, G.; Holban, A.; Ilie, F.; Irimia, A.; Nicoara, I.; Tone, M. Nano-hydroxyapatite: Novel approaches in biomedical applications. Nano Biomater. Hard Tissue Eng. 2016, 4, 189–213. Wang, H.X.; Guan, S.K.; Wang, X.; Ren, C.X.; Wang, L.G. In vitro degradation and mechanical integrity of Mg-Zn-Ca alloy coated with Ca-deficient hydroxyapatite by the pulse electrodeposition process. Acta Biomater. 2010, 6, 1743–1748. [CrossRef] Zainol, I.; Alwi, N.M.; Abidin, M.Z.; Haniza, H.M.Z.; Ahmad, M.S.; Ramli, A. Physicochemical properties of hydroxyapatite extracted from fish scales. J. Adv. Mater. Res. 2012, 545, 239–253. Burnat, B.; Walkowiak-Przybyło, M.; Błaszczyk, T.; Klimek, L. Corrosion behaviour of polished and sandblasted titanium alloys in PBS solution. Acta Bioeng. Biomech. 2013, 15, 87–95. [PubMed] Kokubo, T.; Takadama, H. How useful is SBF in predicting in vivo bone bioactivity? Biomaterials 2006, 27, 2907–2915. [CrossRef] ASTM F1926/F1926M; Standard Test Method for Dissolution Testing of Calcium Phosphate Granules, Fabricated Forms, and Coatings. ASTM International: West Conshohocken, PA, USA, 2021. Wolf, M.F.; Coleman, K.P.; Lewerenz, G.M. In Vitro Assessment of Cell and Tissue Compatibility. In Biomaterials Science: An Introduction to Materials in Medicine, 3rd ed.; Elsevier Ltd.: Amsterdam, The Netherlands, 2013; pp. 593–608. ISO 10993-5; Biological Evaluation of Medical Devices-Part 5: Tests for In Vitro Cytotoxicity. International Organization for Standardization: Vernier Geneva, Switzerland, 2009. ISO 13779-2008; Implants for Surgery-Hydroxyapatite Part 3: Chemical Analysis and Characterization of Crystallinity and Phase Purity. International Organization for Standardization: Geneva, Switzerland, 2008. Tanya, J.L. Optimisation of Plasma Sprayed Hydroxyapatite Coatings. Ph.D. Thesis, Dublin City University, Dublin, Ireland, 2008. Lee, T.M.; Tsai, R.S.; Chang, E.; Yang, C.Y.; Yang, M.R. The cell attachment and morphology of neonatal rat calvarial osteoblasts on the surface of Ti6Al4V and plasma sprayed HA coating: Effect of surface roughness and serum contents. J. Mater. Sci. Mater. Med. 2002, 13, 341–350. [CrossRef] Liu, J.; Long, X.; Zhu, H.; Zhu,W.; Chen, Z.; He, D.; Song, N.;Wang, X. Effects of La2O3 contents on microstructure and properties of laser-cladded 5 wt.% CaB6/HA bioceramic coating. Biomed. Mater. 2022, 17, 025007. [CrossRef] Singh, G.; Singh, H.; Sidhu, B.S. Corrosion behavior of plasma sprayed hydroxyapatite and hydroxyapatite-silicon oxide coatings on AISI 304 for biomedical application. Appl. Surf. Sci. 2013, 284, 811–818. [CrossRef] Cizek, J.; Aik Khor, K. Role of in-flight temperature and velocity of powder particles on plasma sprayed hydroxyapatite coating characteristics. Surf. Coat. Technol. 2012, 206, 2181–2191. [CrossRef] Sun, L.; Berndt, C.C.; Gross, K.A.; Kucuk, A. Material fundamentals and clinical performance of plasma-sprayed hydroxyapatite coatings: A review. J. Biomed. Mater. Res. 2001, 58, 570–592. [CrossRef] Anene, F.A.; Jaafar, C.N.A.; Zainol, I.; Hanim, M.A.A.; Suraya, M.T. Evaluation of Corrosion Inhibition of Plasma Sprayed FsHA/YSZ Coating on -Titanium (Ti-13Nb-13Zr) Alloy Using Electrochemical Techniques. J. Biomim. Biomater. Biomed. Eng. 2022, 58, 45–57. [CrossRef] Tlotleng, M.; Akinlabi, E.; Shukla, M.; Pityana, S. Microstructures, hardness and bioactivity of hydroxyapatite coatings deposited by direct laser melting process. Mater. Sci. Eng. C 2014, 43, 189–198. [CrossRef] Hou, B.; Liua, Y.; Chena, H.; Yang, Y. In vitro Bioactivity, Bio-Corrosion Resistance and Antibacterial Property of Laser Cladded HA Coatings with Different Content of ZnO on Ti-6Al-4V Substrate. Mater. Res. 2019, 22 (Suppl. S2), e20180744. [CrossRef] Ahmed, Y.; Rehman, M.A.U. Improvement in the surface properties of stainless steel via zein/hydroxyapatite composite coatings for biomedical applications. J. Surf. 2020, 20, 589–624. [CrossRef] Solanke, S.; Gaval, V. Most commonly used metallic biomaterials for plasma sprayed hydroxyapatite coatings. IOP Conf. Ser. Mater. Sci. Eng. 2021, 1168, 012013. [CrossRef] Ning, C.Q.; Zhou, Y. In vitro bioactivity of a biocomposite fabricated from HA and Ti powders by powder metallurgy method. Biomaterials 2002, 23, 2909–2915. [CrossRef] [PubMed] Guo, H.; Murakami, H.; Kuroda, S. Effect of hollow spherical powder size distribution on porosity and segmentation cracks in thermal barrier coatings. J. Am. Ceram. Soc. 2006, 89, 3797–3804. [CrossRef] |
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