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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| The objectives of this study are to synthesize novel chitosan-hydroxyapatite composites doped with strontium, magnesium and zinc via in situ co-precipitation method, to investigate the bioactivity, cytotoxicity and antimicrobial properties of the materials. Physicochemical properties of the products obtained were analysed by X-ray powder diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential thermal analysis (DTA), field emission scanning electron microscopy (FESEM) and energy dispersive X-ray spectroscopy (EDX). The calcium release from the samples in simulated body fluid (SBF) were measured using atomic absorption spectroscopy (AAS). The synthesized composite materials were subjected to bioactivity studies in SBF. In vitro cytotoxicity studies were performed on the novel composite materials obtained. Antimicrobial activity investigations against E. coli were carried out on all the composite samples. Characterization of the composite samples revealed that three novel composites, viz; chitosan-hydroxyapatite composite doped with strontium, chitosan-hydroxyapatite composites doped with magnesium and chitosan-hydroxyapatite composite doped with zinc were successfully synthesized. All the composites obtained showed good bioactivity in SBF. The morphologies of the composites obtained by FESEM analysis revealed that they have good apatite forming ability in SBF. The calcium/phosphorous ratio (Ca/P) of the composites calculated after soaking in SBF have values that are comparable to that of natural apatite. Chitosan-hydroxyapatite composites doped with strontium and zinc were found to be non-cytotoxic against the cell culture used. Chitosan-hydroxyapatite composite doped with magnesium was found to be mildly cytotoxic. Chitosan-hydroxyapatite composites doped with strontium and magnesium show no antimicrobial effects against the E. coli bacteria. Chitosan-hydroxyapatite composite doped with zinc show substantial antibacterial effect against E. coli. The novel composites obtained thus far can be used potentially for bone grafting, bone filler and template for bone tissue engineering. |
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