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Type :thesis
Subject :QR180 Immunology
Main Author :Nor Asyikin Kamarazaman
Title :Characterization of major allergens of selective local snails and their cross-reactivity against common shellfish (IR)
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2017
Notes :Masters
Corporate Name :Universiti Pendidikan Sultan Idris
PDF Full Text :The author has requested the full text of this item to be restricted.

Abstract : Universiti Pendidikan Sultan Idris
This study aimed to identify the major allergens of two species of snails, Cerithidea obtusa (C. obtusa) and Pila polita (P. polita). C. obtusa and P. polita samples were obtained from Perak and Kelantan, respectively.Subsequently, the cross-reactive allergens between C. obtusa and P. polita with common local shellfish including Penaeus monodon (P. monodon), Loligo edulis (L. edulis) dan Scylla serrata (S. serrata) were identified. Proteins of C. obtusa, P. polita, P. monodon, L. edulis and S. serrata were extracted from their flesh. Protein profiles were determined by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). Major and cross-reactive allergens were then identified by immunoblotting and immunoblotting inhibition tests, respectively using sera from patients with allergy to C. obtusa, P. polita, P. monodon, L. edulis dan S. serrata. The major allergens of C. obtusa and P. polita were then identified by mass spectrometry analysis. Research findings showed that C. obtusa and P. Polita extracts contain 31 bands between 9 to 240 kDa and 24 bands between 9 to 245 kDa, respectively. Immunoblotting detected three major allergens in C. obtusa and four in P. polita. A 49 kDa band was identified as the major allergen in both snails, while other major allergens at 33 and 42 kDa, and 30, 35 and 42 kDa were identified in C. obtusa and P. polita, respectively. Mass spectrometry analysis identified the 49 kDa protein band of both snails and the 42 kDa band of C. obtusa as actin. Meanwhile, the major allergens of 33 kDa of C. obtusa and the 30 and 35 kDa of P. polita were identified as tropomyosin. Immunoblotting inhibition tests demonstrated the existence of cross-reactivity among major and minor allergens of both snails,with either complete or partial inhibitions. In addition, cross-reactivity between C. obtusa dan P. polita against P. monodon, L. edulis and S. serrata allergens was also revealed. As a conclusion, actin and tropomyosin of both species of snails were identified as the major and cross-reactive allergens among local snail-allergic patients. The implication of this study is to provide insights of local snail allergens to improve strategy of diagnosis and management of snail allergic patients in this country.


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