Anticancer effect of hypophyllanthin, niranthin and lintetralin from phyllanthus amarus on HeLa cells and NIH/3T3 cells.

Mohd Azlan Nafiah

QR Code Link :
Type :	article
Subject :	R Medicine (General)
Main Author :	Mohd Azlan Nafiah
Additional Authors :	Nur Affira Mohd Noor Syed Ahmad Tajudin Tuan Johari Muhammad Hafiz Husna Hasnan Tan, Siow-Ping Liew, Sook Yee Unang Supratman
Title :	Anticancer effect of hypophyllanthin, niranthin and lintetralin from phyllanthus amarus on HeLa cells and NIH/3T3 cells.

Place of Production :	Tanjong Malim
Publisher :	Fakulti Sains dan Matematik
Year of Publication :	2019
Corporate Name :	Universiti Pendidikan Sultan Idris

Abstract : Universiti Pendidikan Sultan Idris

Phyllanthus amarus which is locally known as Dukung Anak was one of the herb used in traditional medicine and research of P. amarus in Malaysia was not widely reported. This present research has isolated three chemical compounds and its anticancer effect of this species has been determined. Three lignans namely hypophyllanthin was afforded from hexane crude while niranthin and lintetralin were afforded from ethanol crude. Anticancer test against HeLa cells and NIH/3T3 cells by MTT assays was tested for its anticancer effect. The result shows that hypophyllanthin was considered to possess an active anticancer effect on HeLa cells than NIH/3T3 cells compared to niranthin and lintetralin.

References

1. T. O. Ajala, C. I. Igwilo, I. A. Oreagba, O. A. Odeku, “The antiplasmodial effect of the extracts and formulated capsules of Phyllanthus amarus on plasmodium yoelii infection in mice,” Asian Pacific Journal of Tropical Medicine, vol. 4, 2011, pp. 283-287.

2. A. H. M. Mahbubur Rahman, M. Akter, “Taxonomy and medicinal uses of Euphorbiaceae (Spurge) family of Rajshahi, Bangladesh,” Research in Plant Sciences, vol. 1, no.3, 2013, pp. 74-80.

3. S. Prasad M, S. Joshi DSD, K. Narendra, K. Srinivas, S. Bai J, L. Chandana M, K. Satya A, “Phytochemical and pharmacological evaluation of Euphorbiaceae family plant leaves- Acalypha Indica L., Croton Bonplandianum Baill,” Mintage Journal of Pharmaceutical & Medical Science, vol. 4, no. 3, 2016, pp. 17-22.

4. A. K. Tripathi, R. K. Verma, A. K. Gupta, M. M. Gupta, S. P. S. Khanuja, “Quantitative determination of phyllanthin and hypophyllanthin in Phyllanthus species by high-performance thin layer chromatography, ”Phytochemical Analysis, vol. 17, 2006, pp. 394-397.

5. A. Adewale Adeneye, A. Senebo Benebo, “Protective effect of the aqueous leaf and seed extract of Phyllanthus amarus on gentamicin and acetaminophen-induced nephrotoxic rats,” Journal of Ethnopharmacology, vol. 118, 2008, pp. 318-323.

6. B. Joseph and S. Justin Raj, “A comparitive study on various properties of five medicinally important plants, ”International Journal of Pharmacology, vol. 7, no. 2, 2011, pp. 206-211.

7. B. Joseph and S. Justin Raj, “An overview: pharmacognostic properties of Phyllanthus amarus Linn, ”International Journal of Pharmacology, vol.7, no. 1, 2011, pp. 40-45.

8. S. Khatoon, “Macro-microscopy & planar chromatography-important tools for quality control and identification of adulterants/substitutes of unani drugs,” International Journal of Advances in Pharmacy Medicine and Bioallied Sciences, vol. 3, no. 1, 2015, pp. 58-64.

9. J. Ram Patel, P. Tripathi, V. Sharma, N. Singh Chauhan and V. Kumar Dixit, “ Phyllanthus amarus: Ethnomedicinal uses, phytochemistry and pharmacology: A review,” Journal of Ethnopharmacology, vol. 138, no. 2, 2011, pp. 286-313.

10. S. Verma, H. Sharma and M. Garg, “ Phyllanthus amarus : A review” Journal of Pharmacognosy and Phytochemistry, vol. 3, no. 2, 2014, pp.18-22.

11. A. A. Adeneye, “The leaf and seed aqueous extract of Phyllanthus amarus improves insulin resistance diabetes in experimental animal studies,” Journal of Ethnopharmacology, vol. 144, no 3, 2012, pp. 705-711.

12. A. Islam, T. Selvan, U. K. Mazumder, M. Gupta and S. Ghosal, “Antitumour Effect of phyllanthin and hypophyllanthin from Phyllanthus amarus against ehrlich ascites carcinoma in mice,” Pharmacologyonline, vol. 2, 2008, pp. 796-807.

13. A. D. Kandhare, P. Ghosh, A. E. Ghule, G. N. Zambare and S. L. Bodhankar, “ Protective effect of Phyllanthus amarus by modulation of endogenous biomarkers and DNA damage in acetic acid induced ulcerative colitis?: Role of phyllanthin and hypophyllanthin,” Apollo Medicine, vol. 10, no. 1, 2013, pp. 87 97.

14. C. A. L. Kassuya, A. A. Silvestre, V. L. G. Rehder and J. B. Calixto, “Anti-allodynic and anti-oedematogenic properties of the extract and lignans from Phyllanthus amarus in models of persistent inflammatory and neuropathic pain,” European Journal of Pharmacology, vol. 478, 2003, pp. 145-153.

15. P. M. Kiran, and B. G. Rao, “Evaluation of anti-inflammatory activity of different extracts and isolated lignans of Phyllanthus amarus Schum. & Thonn. aerial parts,” International Journal of Pharma and Bio Sciences, vol. 4, no. 2, 2013, pp. 803-808.

16. B. Sarin, N. Verma, J. Pedro Martin and A. Mohanty, “An overview of important ethnomedicinal herbs of Phyllanthus species: Present status and future prospects,” The Scientific World Journal, 2014, pp. 1-12.

17. U. T. Mamza, O. A. Sodipo and I. Z. Khan, “Gas chromatography-mass spectrometry?: Analysis of bioactive components of Phyllanthus amarus leaves,” International Research Journal of Plant Science, vol. 3, no. 10, 2012, pp. 208-215.

18. G. Abhyankar, K. V. Rao and V. D. Reddy, “Genomic and metabolomic fingerprinting of Phyllanthus amarus (Schumm & Thonn) hairy root clones,” Annals of Phytomedicine, vol. 2, no. 1, 2013, pp. 74-88.

19. G. Abhyankar, P. Suprasanna, B. N. Pandey, K. P. Mishra, K. V. Rao and V. D. Reddy, “Hairy root extract of Phyllanthus amarus induces apoptotic cell death in human breast cancer cells,” Innovative Food Science and Emerging Technologies, vol. 11, no. 3, 2010, pp. 526-532.

20. R. Krithika, R. J. Verma, P. S. Shrivastav and L. Suguna, “Phyllanthin of standardized Phyllanthus amarus extract attenuates liver oxidative stress in mice and exerts cytoprotective activity on human hepatoma cell line,” Journal of Clinical and Experimental Hepatology, vol. 1, no. 2, 2011, pp. 57-67.

21. B. Sparzak, M. Krauze-Baranowska, A. Kawiak, P. Sowinski, “Cytotoxic lignan from the non-transformed root culture of Phyllanthus amarus,” Molecules, vol. 20, no. 5, 2015, pp. 7915-7924.

22. J. S. Thakur, R. K. Agarwal and M. D. Kharya, “Immobilization mediated enhancement of phyllanthin and hypophyllanthin from Phyllanthus amarus,” Chinese Journal of Natural Medicines, vol. 10, no.3, 2012, pp. 207-212.

23. N. P. Yadav, A. Pal, K. Shanker, D. U. Bawankule, A. K. Gupta, M. P. Darokar and S. P. S. Khanuja, “Synergistic effect of silymarin and standardized extract of Phyllanthus amarus against CCl 4 -induced hepatotoxicity in Rattus norvegicus,” Journal of Phytomedicine, vol. 15, 2008, pp. 1053-1061.

24. S. Liu, W. Wei, K. Shi, X. Cao, M. Zhou, Z. Liu, “In vitro and in vivo anti-hepatitis B virus activities of the lignan niranthin isolated from Phyllanthus niruri L.,” Journal of Ehnopharmacology, vol. 155, no. 2, 2014, pp. 1061-1067.

25. M. A. M. Maciel, A. F. Cunha and T. N. C. Dantas, “NMR characterization of bioactive lignans from Phyllanthus amarus Schum & Thorn., Annals of Magnetic Resonance, vol. 6, no. 3, 2007, pp. 76-82

This material may be protected under Copyright Act which governs the making of photocopies or reproductions of copyrighted materials.
You may use the digitized material for private study, scholarship, or research.

Back to previous page