UPSI Digital Repository (UDRep)
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Abstract : Universiti Pendidikan Sultan Idris |
The work presented in this thesis evaluates the status of Peninsular Malaysian
Cyatheaceae and used molecular and morphological identification tools for the local
species. 419 Cyatheaceae frond sample were collected from the widest possible range
of Peninsular Malaysia to obtain material for morphology and molecular study. 15
Cyathea species were identified and the species information for Peninsular Malaysia
was updated. The species was incorporated into the existing Cyatheaceae phylogeny
by using four plastid regions: matK, rbcL, trnG-trnR and trnL-trnF. Bayesian MCMC
analysis of the concatenated sequence data resulted in a 50% majority rule consensus
tree confirm the placement of the four groups: Cyathea, Alsophila, Gymnosphaera
and Sphaeropteris in the family. However, the resulting tree representing nested
monophyletic groups, proposing Cyatheaceae to be monogeneric, i.e., Cyathea with
two large groups: Cyathea and Sphaeropteris. The same plastid regions were then
evaluated to develop DNA barcodes. trnL-trnF was proposed as a barcode for this
family as it almost satisfied the three most important criteria: primer universality,
sequence quality and species discrimination. This research also developed a multiaccess
key for Cyatheaceae field identification based on fifteen taxa identified, by
extensive field sampling of the currently recognised species. All of the Cyatheaceae
species recognises in this study had also been assessed for the conservation status
based on the IUCN Red List criteria. Nine species fall under Least Concern (LC), four
species are Near Threatened (NT) and two species are Vulnerable (VU). The thorough
knowledge regarding Cyatheaceae in Peninsular Malaysia gained through the work
done in this research will benefit in making appropriate conservation strategies for the
survival of this family. Overall, the most important outcome of this research was the
combination of morphology and molecular data for the purpose of updating
taxonomy, identification and conservation of the Cyatheaceae family in Peninsular
Malaysia |
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