UPSI Digital Repository (UDRep)
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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| The study aims to investigate the effect of photo-autotrophic cultural conditions on the productivity and biomass composition of Chlorella vulgaris. The following five photo-autotrophic cultural conditions were investigated: light intensity (4000, 10,500, 17,000, 23,000, 30,000 lux), temperature (25, 28, 32, 35, 40 C), pH (6, 7, 8, 9, 10), CO2 (0.03, 2.5, 5, 7.5, 10%) and NO3 (0, 250, 500, 750, 1000 mg/L). Results indicate that lipid and protein yields were increased by 3.19 fold and decreased by 1.47 fold, respectively, under NO3 deficiency (0 mg/L). Meanwhile, carbohydrate yield was increased by 1.39 fold in 5% CO2. Further cultivation with parameters of the highest biomass productivity (10,500 lux, 28 C, pH 8, 5% CO2 and 500 mg/L NO3 ) achieved the maximum biomass productivity of 0.468 g/L/day. Moreover, cultivation with parameters of the highest lipid yield (23,500 lux, 40 C, pH 8, 0.03% CO2 and 0 mg/L NO3) achieved the maximum lipid yield of 43.70%. The major fatty acid methyl ester compositions produced were C20:0 (39.08%) and C16:0 (37.15%), producing biodiesel with high cetane number and oxidative stability. These promising results provide a comprehensive comparison regarding the effect of photo-autotrophic cultural conditions on microalgae biomass and its potential application as a biofuels feedstock. |
| References |
[1] Vello V, Phang SM, Chu WL, et al. Lipid productivity and fatty acid composition-guided selection of Chlorella strains isolated from Malaysia for biodiesel production. J Appl Phycol. 2014;26: 1399–1413. [2] Mujtaba G, Choi W, Lee CG, et al. Lipid production by Chlorella vulgaris after a shift from nutrient-rich to nitrogen starvation conditions. Bioresour Technol. 2012;123:279–283. [3] Daliry S, Hallajisani A, Roshandeh JM, et al. Investigation of optimal condition for Chlorella vulgaris microalgae growth. Glob J Environ Sci Manage. 2017;3:217–230. [4] Shen XF, Liu JJ, Chauhan AS, et al. Combining nitrogen starvation with sufficient phosphorus supply for enhanced biodiesel productivity of Chlorella vulgaris fed on acetate. Algal Res. 2016;17:261–267. [5] Robles-Heredia JC, Sacramento-Rivero JC, Canedo-Lopez Y, et al. A multistage gradual nitrogen reduction strategy for increased lipid productivity and nitrogen removal in wastewater using Chlorella vulgaris and Scenedesmus obliquus. Braz J Chem Eng. 2015;32:335–345. [6] Xin L, Hu H, Ke G, et al. Effects of different nitrogen and phosphorus concentrations on the growth, nutrient uptake, and lipid accumulation of a freshwater microalga Scenedesmus sp. Bioresour Technol. 2010;101:5494–5500. [7] Prakash M, Rai MP, Gautom T, et al. Effect of salinity, pH, light intensity on growth and lipid production of microalgae for bioenergy application. Online J Biol Sci. 2015;15:260–267. [8] Raeesossadati MJ, Ahmadzadeh H, McHenry MP, et al. CO2 bioremediation by microalgae in photobioreactors: impacts of biomass and CO2 concentrations, light, and temperature. Algal Res. 2014;6:78–85. [9] Takeshita T, Ota S, Yamazaki T, et al. Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresour Technol. 2014;158:127–134. [10] Ho SH, Chen CY, Chang JS. Effect of light intensity and nitrogen starvation on CO2 fixation and lipid/carbohydrate production of an indigenous microalga Scenedesmus obliquus CNW-N. Bioresour Technol. 2012;113:244–252. [11] Nordin N, Yusof N, Samsudin S. Biomass production of Chlorella sp., Scenedesmus sp., and Oscillatoria sp. in nitrified landfill leachate. Waste Biomass Valorization. 2017;8:2301–2311. [12] Ganapathi S, Shetty V, Mokashi K. Enhanced lipid productivity approaches in microalgae as an alternate for fossil fuels: a review. J Energy Inst. 2015;89:1–5. [13] Safi C, Zebib B, Merah O, et al. Morphology, composition, production, processing and applications of Chlorella vulgaris: a review. Renew Sustain Energy Rev. 2014;35:265–278. [14] Markou G, Depraetere O, Vandamme D, et al. Cultivation of Chlorella vulgaris and Arthrospira platensis with recovered phosphorus from wastewater by means of zeolite sorption. Int J Mol Sci. 2015;16:4250–4264. [15] Suthar S, Verma R, Kumar K. Production of Chlorella vulgaris under varying nutrient and abiotic conditions: a potential microalga for bioenergy feedstock. Process Saf Environ Prot. 2018;113:141–148. [16] Neves B, Jacob-Lopes E, Franco TT. Microalgae as feedstock for biodiesel production: carbon dioxide sequestration, lipid production and biofuel quality. J Chem Technol Biotechnol. 2010; 85:395–403 [17] Fung KS, Liew EWT, Ngu HLN. Optimization of nutrient media composition for microalgae biomass production using central composite design. In: Wang L, editors. Proceeding of Chemeca 2013: Challenging Tomorrow; 2013; Barton, ACT: Engineers Australia; 2013. p. 278–282. [18] Hong SJ, Lee CG. Statistical optimization of culture media for production of phycobiliprotein by Synechocystis sp. PCC 6701.Biotechnol Bioprocess Eng. 2008;13:491–498. [19] Ho SH, Huang SW, Chen CY, et al. Characterization and optimization of carbohydrate production from an indigenous microalga Chlorella vulgaris FSP-E. Bioresour Technol. 2013;135:157–165. [20] Nan Y, Liu J, Lin R, et al. Production of biodiesel from microalgae oil (Chlorella protothecoides) by non-catalytic transesterification in supercritical methanol and ethanol: process optimization. J Supercrit Fluids. 2015;97:174–182. [21] Tuantet K, Temmink H, Zeeman G, et al. Nutrient removal and microalgal biomass production on urine in a short light-path photobioreactor. Water Res. 2014;5:162–174. [22] Blair MF, Kokabian B, Gude VG. Light and growth medium effect on Chlorella vulgaris biomass production. J Environ Chem Eng. 2014;2:665–674. [23] Li L, Cui J, Liu Q, et al. Screening and phylogenetic analysis of lipid-rich microalgae. Algal Res. 2015;11:381–386. [24] Zhu S, Huang W, Xu J, et al. Metabolic changes of starch and lipid triggered by nitrogen starvation in the microalga Chlorella zofingiensis. Bioresour Technol. 2014;152:292–298. [25] Ramos MJ, Fernandez CM, Casas A, et al. Influence of fatty acid composition of raw materials on biodiesel properties. Bioresour Technol. 2009;100:261–268. [26] Krisnangkura K. A simple method for estimation of cetane index of vegetable oil methyl esters. J Am Oil Chem Soc. 1986; 63:552–553. [27] Talebi AF, Mohtashami SK, Tabatabaei M, et al. Fatty acids profiling: a selective criterion for screening microalgae strains for biodiesel production. Algal Res. 2013;2:258–267. [28] Arora N, Patel A, Pruthi PA, et al. Synergistic dynamics of nitrogen and phosphorous influences lipid productivity in Chlorella Minutissima for biodiesel production. Bioresour Technol. 2016; 213:79–87. [29] Su YC, Liu YA, Diaz Tovar CA, et al. Selection of prediction methods for thermophysical properties for process modeling and product design of biodiesel manufacturing. Ind Eng Chem Res. 2011;50:6809–6836. [30] Barghbani R, Rezaei K, Javanshir A. Investigating the effects of several parameters on the growth of Chlorella vulgaris using Taguchi’s experimental approach. Int J Biotechnol Wellness Ind. 2012;1:128–133. [31] Converti A, Casazza AA, Ortiz EY, et al. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem Eng Process Process Intensif. 2009;48:1146–1151. [32] Binnal P, Babu PN. Statistical optimization of parameters affecting lipid productivity of microalga Chlorella protothecoides cultivated in photobioreactor under nitrogen starvation. South African J Chem Eng. 2017;23:26–37 [33] Bidle KD, Falkowski PG. Cell death in planktonic photosynthetic microorganisms. Nat Rev Microbiol. 2004;2:643–655. [34] Zuppini A, Andreoli C, Baldan B. Heat stress: an inducer of programmed cell death in Chlorella saccharophila. Plant Cell Physiol. 2007;48:1000–1009. [35] Serra-Maia R, Bernard O, Gonzalves A, et al. Influence of temperature on Chlorella vulgaris growth and mortality rates in a photobioreactor. Algal Res. 2016;18:352–359. [36] Nguyen TDP, Frappart M, Jaouen P, et al. Harvesting Chlorella vulgaris by natural increase in pH: effect of medium composition. Environ Technol. 2014;35:1378–1388. [37] Kumar A, Ergas S, Yuan X, et al. Enhanced CO2 fixation fixation and biofuel production via microalgae: recent developments and future directions. Trends Biotechnol. 2010;28:371–380. [38] Thawechai T, Cheirsilp B, Louhasakul Y, et al. Mitigation of carbon dioxide by oleaginous microalgae for lipids and pigments production: effect of light illumination and carbon dioxide feeding strategies. Bioresour Technol. 2016;219:139–149. [39] Singh A, Olsen SI. A critical review of biochemical conversion, sustainability and life cycle assessment of algal biofuels. Appl Energy. 2011;88:3548–3555. [40] Li T, Gargouri M, Feng J, et al. Regulation of starch and lipid accumulation in a microalga Chlorella sorokiniana. Bioresour Technol. 2015;180:250–257. [41] Abdelaziz AEM, Leite GB, Belhaj MA, et al. Screening microalgae native to Quebec for wastewater treatment and biodiesel production. Bioresour Technol. 2014;157:140–148. [42] Fan J, Xu H, Luo Y, et al. Impacts of CO2 concentration on growth, lipid accumulation, and carbon-concentrating-mechanism-related gene expression in oleaginous Chlorella. Appl Microbiol Biotechnol. 2015;99:2451–2462. [43] Battah M, El-Ayoty Y, El-Fatah SA, et al. Optimization of growth and lipid production of the chlorophyte microalga Chlorella vulgaris as a feedstock for biodiesel production. World Appl Sci J. 2013;28:1536–1543. [44] Ram?rez-Lopez C, Chairez I, Fern andez-Linares L. A novel culture medium designed for the simultaneous enhancement of biomass and lipid production by Chlorella vulgaris UTEX 26. Bioresour Technol. 2016;212:207–216. [45] Hu Q, Zeng R, Zhang SX, et al. Production of microalgal lipids as biodiesel feedstock with fixation of CO2 by Chlorella vulgaris. Food Technol Biotechnol. 2014;52:285–291. [46] Shekh AY, Shrivastava P, Krishnamurthi K, et al. Stress enhances poly-unsaturation rich lipid accumulation in Chlorella sp. and Chlamydomonas sp. Biomass Bioenergy. 2016;84:59–66. [47] Tale M, Ghosh S, Kapadnis B, et al. Isolation and characterization of microalgae for biodiesel production from Nisargruna biogas plant effluent. Bioresour Technol. 2014;169:328–335. [48] Sinha SK, Gupta A, Bharalee R. Production of biodiesel from freshwater microalgae and evaluation of fuel properties based on fatty acid methyl ester profile. Biofuels. 2016;7:69–121. [49] Rawat I, Ranjith KR, Mutanda T, et al. Biodiesel from microalgae: a critical evaluation from laboratory to large scale production. Appl Energy. 2013;103:444–467.
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