UPSI Digital Repository (UDRep)
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Abstract : Universiti Pendidikan Sultan Idris |
The purpose of this study was to examine the propagation ability of Punica granatum
L. through tissue culture system. In addition, callus induction was also carried out in the study
to observe the rate of cell differentiation on explants. The experimental design that was used in
this research is Completely Randomized Design (CRD). The complete regeneration of the Punica
granatum L. plant and callus production was successfully produced through the tissue culture
technique. Explants used in this study were leaves, stem and roots from 8-week-old aseptic
seedlings. All cultures were stored at a temperature of 25 ± 1ºC and light 16 hours of light,
8 hours dark. Plant acclimatization was done on three different medium, garden soil, coco peat and
vermiculite. All data was recorded and analyzed using ANOVA. Leaf explants were found to
be very responsive and Murashige and Skoog (MS) medium added with 2.0 mg/l
Benzylaminoupurine (BAP) + 1.5 mg/l Napthalene Acetic Acid (NAA) has been identified as
the optimum medium for shoot regeneration. Leaf explant managed to produce the number of
shoots in vitro with 1.3333±0.3228 per explant at week 8. Whereas from stem explant, MS
media added with 0.5 mg/l BAP and 2.0 mg/l NAA gave the highest shoot development with 1.0000
± 0.2837 shoots per explant. Combination of NAA and BAP, at 1.5 mg/l respectively was
used for complete regeneration of Punica granatum L. in vitro. In addition, artificial
seeds of Punica granatum were also produced. As a conclusion, this method was proven to be
successful in producing new generation of Punica granatum L. and its features are preserved. This
proves that plant tissue culture technology could be an alternative solution to achieve
high quality of Punica granatum L, therefore could increase the crop production.
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References |
Abd El-Gawad, N.A., N.S. Zaied and M.A. Saleh, 2010. A comparative study on different carbon source concentrations and gelling agent on in vitro proliferation of pineapple (Ananas comosus). The Journal of Nat we and Science, 8(2): 1-5
Acheapong E. (1982). Multiplication and Conservation of Cocoyam (Xarthosoma sajiltifoljum) germplasm by application of tissue culture methods Ph.D Thesis. University of Burmingham, UK
Akoroda M. O. (1990). Ethno botany of Telfaria Occidtendalic Among Igbos of Nigeria. Econ. Bot. 44 (1): 29-39.
Aladele S. E. and Kuta D. (2008). Environmental and genotypic effects on the rowth rate of in vitro cassava plantlet (Manihot esceulenta) African Journal of Biotechnology Vol. 7 (4), pp. 381-385.
Al-Khateeb, A.A., (2008). Regulation of in vitro bud formation of date palm (Phoenix dactylifera L.) cv. Khanezi by different carbon sources. Bioresource Technology, 99(14): 6550-6555.
Al-Khateeb, A.A., (2008). Regulation of in vitro bud formation of date palm (Phoenix dactylifera L.) cv. Khanezi by different carbon sources. Bioresource Technology, 99(14): 6550-6555.
Al-Khayri, (2003). In vitro germination of somatic embryos in date palm : Effect of auxin concentration and strength of MS salts. Current Science, 84(3): 680-683.
Amin MN, Islam MN, Azad MAK (1999). Regeneration of plantlets in vitro from the seedling explants of pomegranate (Punica granatum L.). Plant Tissue Cult. 9(1): 53-61.
Andreu, P., J.A. Marín (2005). In vitro culture establishment and multiplication of the Prunus rootstock Adesoto 101’ (P. insititia L.) as affected by the type of propagation of the donor plant and by the culture medium composition. Scientia Horticulturae, 106(2): 258-267.
Ara H, Jaiswal Uma, Jaiswal VS ( 2000 ) Synthetic seed: Prospects and limitations, Current Science 78, 1438 – 1444
Attree, S.M.; Pomeroy, M.K.; Fowke, L.C. ( 1994 ) Production of vigoous, dessication tolerant white spruce (Picea-glauca (moench) voss) synthetic seeds in a bioreactor. Plant Cell Rep., 13, 601–606.
Baaya, M.H.M. (2002). Studies on propagation of some fruit species by using tissue culture techniques. M. Sci. Thesis, Hort. Dept., Fac. of Agric., Moshtohor, Zagazig University.
Babak V.K, Ahmad E. And Masoud T. ( 2013 ) In Vitro Propagation of Pomegranate ( Punica granatum ) Cv. ‘Males Yazdi’. Deparments of Horticulture Science, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran.
Babbar, S.B. and S.C. Gupta (1986) . Effect of Carbon Source on Datura metel Microspore Embryogenesis and the Growth of Callus Raised from Microspore-derived Embryos. Biochemie und Physiologie der Pflanzen, 181(5): 331-338.
Bhatia P, Ashwath N (2005). Effect of medium pH on shoot regeneration from the cotyledonary explants of Tomato. Biotechnol., 4: 7-10.
Bridgen, Mark P. (1994). "A Review of Plant Embryo Culture". Hortscience 29: 1243– 1246.
Brown DCW, Leung DWM, Thorpe TA (1979). Osmotic requirement for shoot formation in tobacco callus. Physiol. Planta, 46: 36-41.
Chang, P.Y, Fujita, S., Kohno, K., Ashrafuzzaman, M.D., Nakamura, N. And Hayashi, N. (2000) Purification and characterization of polyphenol oxidase from banana ( Musa sapientum L.) pulp.Journal of Agricultural and Food Chemistry 48: 2732-2735.
Christian Raquin (1983). Utilization of Different Sugars as Carbon Source for in vitro Anther Culture of Petunia. Zeitschrift für Pflanzenphysiologie, 111(5): 453-457.
Conger, B. V. (1980). Cloning Agricultural Plants via In-vitro Techniques, CRC Press Inc, Boca Raton, Florida, pp; 1-50
Dalmacio I. F. (1992). Biotechnology for sustainable Agriculture National Institute of Biotechnology and Applied Microbiology, University of the Philippines.Dalziel, J. M. 1937. The useful plants of West Tropical Africa. Whitefriars Press, London, 612 pp.
Dalziel J.M. (1937) The useful plants of West Tropical Africa. Whitefriars press,London. 612pp.
Fagbemi T.N.F., Eleyinmi A.F., Atum H. N., Akpambang O. (2005) Nutritional composition of fermented fluted pumpkin (Telfairiaoccidentalis) seeds for productionof “ogiriugu”. IFT Annual Meeting, July 15-20 – New Orleans, Louisana.
Frusciante, L., Barone, A.,Carputo,D. Ercolano, M.R., Della Rocca, F., & Esposito, S. (2010). Evaluation and use of Plant Biodiversity for Food and Pharmaceutical. Fitoterpia. 71 : 66 – S72.
Fuentes, S.R.L., M.B.P. Calheiros, J. Manetti-Filho and L.G.E. Vieira (2000). The effect of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora. Plant Cell Tiss. Org. Cult., 60: 5-13.
Gamborg, O.L., T. Murshige, T.A. Thorpe and I.K. Vasil (1976). Plant tissue culture media. In vitro, 12(7): 473- 478.
Gautheret, R. J. (1934). Culture du tissus cambial. Comptes Rendus Hebdomadaires des Séances de l'Académie des Sciences, 198, 2195–2196.
Gautheret, R. J. (1935). Recherches sur la culture des tissus végétaux. Ph.D. Thesis, Paris
Gautheret, R. J. (1939). Sur la possibilité de réaliser la culture indéfinie des tissus de tubercules de carotte. Comptes Rendus Hebdomadaires des Séances de l'Académie des Sciences, 208, 118–120.
Gbile Z.O.(1998). Collection, Conservation and Utilization of medicinal plants. In Robert PA, & Janice EA (eds.). Conservation of Plant Genes III: Conservation and Utilization of African Plants. Missouri Botanical Gardens Press. pp. 163-174.
Gibson IS (2000). Plant sugar-response pathways. Part of a complex regulatory web. Plant Physiol., 124: 1532-1539.
Gurel S, Gulsen Y (1998). The effects of different sucrose, agar and pH levels on in vitro shoot production of Almond (Amygdalus communis L.). Tr. J. Bot., 22: 363-373.
Haberlandt, G. (1902) Kulturversuche mit isolierten Pflanzenzellen. Sitzungsber. Akad. Wiss. Wien. Math.-Naturwiss. Kl., Abt. J. 111, 69–92.
Hassan, S., All. M. (2012). Studies on improving flowering and fruiting of lecont e pears. Ph.D. Hort., Dept., Fac. of Cairo Univ.
IUCN (2006). IUCN Red List of Threatened Species. IUCN, Gland, Switzerland.
IUCN (2006). IUCN Red List of Threatened Species. IUCN, Gland, Switzerland.
Jain, N., M.W. Bairu, W.A. Stirk, J. Van Staden (2009). The effect of medium, carbon source and explant on regeneration and control of shoot-tip necrosis in Harpagophytum procumbens. South African Journal of Botany, 75(1): 117-121.
M., K. Imizu and T. Hirano (2001). Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear. Scientia Horticulturae, 89(3,4): 207-215.
Kumar HGA Murthy HN and Paek KY (2003 ). Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L. Scientia Hort , 98: 213-222
Levin, R., Stav, R., Alper, Y. and Watad, A. A. (1998). A technique for repeated nonaxenic subculture of plant tissues in a bioreactor on liquid medium containing sucrose. Plant Tissue Culture Biotechnology. (3):41–45.
Lloyd, G. and B.H. McCown 1(981). Commerciallyfeasiblemicropropagation of Mountain Laurel, Kalmia latifolia, by shoot tip culture. Proc. Int. Plant Prop. Soc., 30: 421- 427.
Lyam P.T.,Musa M. L., Jamaleddine, Z.O., Okere, A.U., Carlos A. and Odofin, W.T. (2012). The potentials of Temporary immersion bioreactor TIBS in meeting crop production demand in Nigeria. Journal of biology and life science ISSN 2157 -6076 Vol. 3:1.
Mantel A.H. and Smith H. Culture factors that influence secondary metabo lism accumulation in plat cell and tissue culture. In: Mantell. A., Smith H(Eds). Plant biotechnology, Cambridge University Press, Cambridge, 1983; 75-110.
Mantel S.H., Mathews J.A. and McKee R.A. (1985). Principles of plant Biotechnology,Blackwell Scientific Publications, London.
Miyajuma, D. (2006). "Ovules that failed to form seeds in zinnia (Zinnia violacec Cav)". Sci Hortic 107 (2): 176–182. doi:10.1016/j.scienta.2005.06.014.
Mukherjee, P., N. Husain, S.C. Misra and V.S. Rao (2010). In vitro propagation of a grape rootstock, deGrasset (Vitis champinii Planch.): Effects of medium compositions and plant growth regulators. Scientia Horticulturae, 126(1): 13-19.
Murashige, T. and F. Skoog (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15: 473-497.
Murashige, T. (1973). Nutrition of plant cells and organs in vitro. In Vitro, 9: 81-85.
Murkute, A., Patil, S., Patil, B.N., Mayakumari, M.S., (2002) Micropropagation in pomegranate, callus induction and differentiation. South Indian Hort. 50:49–55.
Musa M. L., Lyam P.T., Jamaleddine, Z.O., Okere, A.U., Carlos A. and Odofin, W.T. (2012). The potentials of Temporary immersion bioreactor TIBS in meeting crop production demand in Nigeria. Journal of biology and life science ISSN 2157 -6076 Vol. 3:1.
Naik, S., Pattnaik, S., Chand, P., (2000) High frequency auxiliary shoot proliferation and plant regeneration from cotyledonary nodes of pomegranate. Sci. Hort. 79:175- 83.
Nehanjali P., Kamlesh K. Ajay Kumar T. (2012 ) In Vitro Organogenesis from Cotyledon Derived Callus Cultures of Punica granatum L. cv. Kandhari Kabuli. National Academy of Science, India.
Nehanjali P., Kamlesh K. Ajay Kumar T. (2013 ) Direct Organogenesis in Punica granatum L. cv. Kandhari Kabuli from Hypocotyl Explants. National Academy of Science, India
Nowak B, Milczzynski K, Hudy L (2004). Sugar uptake and utilization during adventitious bud differentiation on in vitro leaf explants of Wegierka Zwykla plum (Prunus domestica). Plant Cell, Tissue Organ Cult., 76: 255-260.
Okere, A. U. and Adegeye, A. (2011). In vitro propagation of an endangered medicinal timber species Khaya grandifoliola C. Dc. African Journal of Biotechnology Vol. 10(17), pp. 3335-3339.
Omura, M., Matsuta, N., Moriguchi, T., Kuzaki, I., Sanada, T., (1987) Establishment of tissue culture methods in dwarf pomegranate (Punica granatum L. var. ?Nana?) and application for the induction of variants. Bull. Fruit Tree Res. 14:17-44.
Pajimi O. and Fashola T. R. (2010). In vitro micro-propagation of Plukenetia conophora Mull.Arg. Journal of South Pacific Agriculture. 14(1&2):1-5
Petersen, K.K., J. Hansen, A. Bouvet, R. Galvayrac, M. Paques (2001). Significance of different carbon sources and sterilization methods on callus induction and plant regeneration of Miscanthus X ogiformis Honda Giganteus. Plant Cell Tiss. Org. Cult., 58: 189-197.
Redenbaugh, K.; Paasch, B.D.; Nichol, J.W.; Kossler, M.E.; Viss, P.R.;Walker, K.A. ( 1986 ) Somatic seeds-encapsulation of asexual plant embryos. Nat. Biotechnol, 4, 797–801. [CrossRef]
Redenbaugh, K.; Fujii, J.A.; Slade, D ( 1988 ). Encapsulated plant embryos. In Advances in Biotechnological Processes; Mizrahi, A., Ed.; Alan R. Liss Inc.: New York, NY, USA; pp. 225–248.
Redenbaugh, K.; Fujii, J.A.; Slade, D.( 1993 ) Hydrated coating for synthetic seeds. In Synseeds: Application of the Synthetic Seeds to Crop Improvement; CRC Press: Boca Raton, FL, USA, ; pp. 305–327.
Raj D. And Kamlesh K. ( 2010 ). In Vitro Regeneration of Punica granatum plants from different Juvenile Explants. Department of Biotechnology, University of Horticulture and Forestry, India.
Reed, Sandra (2005). Robert N. Trigiano and Dennis J. Gray, ed. Plant Development and Biotechnology. CRC Press. pp. 235–239. ISBN 0-8493-1614-6.
Reinert, J. and P.R. White (1956). The cultivation in vitro of tumor tissues and normal tissues of Picea glauca. Physiol. Plant., 9: 117-189.
Reynolds T.L. Am. J. Bot. 1982; 73: 914-918
Risser, P.G. and P.R. White (1964). Nutritional requirements of spruce tumor cellsin vitro. Physiol. Plant. 17: 620-635.
Sakano K (1990). Proton/phosphate stoichiometry in uptake of inorganic phosphate by cultured cells of Catharanthus roseus (L.) G. Don. Plant Physiol., 93: 479-483.
Shah MI, Jabeen M, Ilahi I. 2003. In v?tro callus induction, its proliferation and regeneration in seed explants of wheat (Triticum aestivum L.) variety Lu-26S. Plant Journal of Botany 35(2), 209- 217.
Sanusi I. S., Odofin W. T,, Aladele S. E, , Olayode M. N., Gamra E. O., Fajimi O. (2008) In vitro culture of Telferia occidentalis under different cytokinins and auxin combination. African journal of biotechnology. 7 (14):2407-2408
Singh N.V., Singh S.K., Patel V.B. 2007. In vitro axillary shoot proliferation and clonal propagation of ‘G-137’ pomegranate (Punica granatum). INDIANJ. AGRIC. SCI. 77(8): 505-508.
Stavarek SJ, Croughan TP, Rains DW (1980). Regeneration of plants from long-term cultures of alfalfa cells. Plant Sci. Lett., 19: 253-261.
Steward, F.C., Mapes, M.O., and Mears, K. (1958). Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells, American Journal of Botany. 45:705-708
Tange, H., Y. Low and C. Lui (2008). Plant regenerate from in vitro leaves of four commercial pyrus species. Plant Soil Environs, 54(4): 140-148.
Teisson, C. and Alvard, D. (1995). A new concept of plant in vitro cultivation in liquid medium: Temporary Immersion. In: Current Issues in Plant Molecular and Cellular Biology.Terzi, M., Cella, R. and Falavigna, A. (Editors). Kluwer Academic Publisher pp
Thomas, D.T & Philip B. (2005). Thidiazuron-induced high frequency shoot organogenesis from leaf-derived callus of a medicinal climber,Tylophora indica (Burm.F) Merrill.In Vitro Cell .Dev.Biol- Plant. 41:124-128.
Vespasiano, B.d.N. and W.C. Otoni (2003). Carbon sources and their osmotic potential in plant tissue culture: does it matter?. Scientia Horticulturae, 97(3-4): 193-202.
White, P.R. (1963). The Cultivation of Animal and Plant Cells. 2nd ed. Ronald Press, New York.
Yang ZH, Ludders P (1993).Organogenesis of Punica granatum L. var.Nana. Angewandte Botanik, 67(5-6): 151-156.
Zhu LW, Zhang SM, Song FS, Gong XM, Fang WJ, Sun J, Li SW (2003). Regeneration system of pomegranate by in vitro culture. Acta. Hort. Sinica, 430(2): 207-208.
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