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Type :thesis
Subject :SB Plant culture
Main Author :Muhammad Al-Amin Mazlan
Title :Plant tissue culture of pomegranate, Punica granatum L.
Place of Production :Tanjong Malim
Publisher :Fakulti Teknikal dan Vokasional
Year of Publication :2018
Corporate Name :Universiti Pendidikan Sultan Idris
PDF Guest :Click to view PDF file

Abstract : Universiti Pendidikan Sultan Idris
The purpose of this study was to examine the propagation ability of Punica granatum L. through tissue culture system. In addition, callus induction was also carried out in the study  to observe the rate of cell differentiation on explants. The experimental design that was used in  this research is Completely Randomized Design (CRD). The complete regeneration of the Punica  granatum L. plant and callus production was successfully produced through the tissue culture  technique. Explants used in this study were leaves, stem  and  roots  from  8-week-old  aseptic   seedlings.  All  cultures  were  stored  at  a temperature of 25 ± 1ºC and light 16 hours of light,  8 hours dark. Plant acclimatization was done on three different medium, garden soil, coco peat and  vermiculite. All data was  recorded  and  analyzed  using  ANOVA.  Leaf  explants  were  found  to   be  very responsive   and   Murashige   and   Skoog   (MS)   medium   added   with   2.0   mg/l  Benzylaminoupurine  (BAP)  +  1.5  mg/l  Napthalene  Acetic  Acid  (NAA)  has  been identified as  the optimum medium for shoot regeneration.  Leaf  explant managed to produce  the  number  of   shoots  in  vitro  with  1.3333±0.3228  per  explant  at  week  8. Whereas from stem explant, MS  media added with 0.5 mg/l BAP and 2.0 mg/l NAA gave  the  highest  shoot  development  with  1.0000   ±  0.2837  shoots  per  explant. Combination  of  NAA  and  BAP,  at  1.5  mg/l  respectively  was   used  for  complete regeneration  of  Punica  granatum  L.  in  vitro.  In  addition,  artificial   seeds  of  Punica granatum were also produced. As a conclusion, this method was proven to be  successful in producing new generation of Punica granatum L. and its features are preserved. This  proves that plant tissue culture technology could be an alternative solution to achieve high quality of Punica granatum L, therefore could increase the crop production.  

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