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Type :thesis
Subject :QR Microbiology
Main Author :Suzana Adenan
Title :Isolation and characterization of new thermophilic aminoacylase from Geobacillus sp. Strain Szn
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2018
Corporate Name :Universiti Pendidikan Sultan Idris
PDF Guest :Click to view PDF file
PDF Full Text :Click to view PDF file

Abstract : Universiti Pendidikan Sultan Idris
Kajian  ini  bertujuan  untuk  memencilkan  dan  mencirikan  aminoasilase  termostabil daripada bakterium termofilik.  Dalam pemencilan bakterium, sampel air dan sedimen telah   diperolehi   dari  Air  Panas  Ulu  Slim,  Perak.  Aminoasilase   SZN  termostabil diekstrak   secara intrasellular dan ditulenkan sehingga homogen dengan menggunakan kromatografi   pertukaran    ion  dan  pengasingan   saiz  dalam  eksperimen   seterusnya. Aminoasilase  SZN yang tulen telah  dicirikan pada pelbagai  suhu, pH, ion logam dan perencat.  Kajian  struktur  lebih  lanjut   menggunakan  spektrokopi  sirkular  dikroisma dilakukan  bagi menentukan  kestabilan  a-heliks dan  lembaran     dalam pelbagai  suhu. Penghasil    aminoasilase    termofilik    yang    dipencilkan     itu    dikenalpasti    sebagai Geobacillus  sp.  strain  SZN.  Aminoasilase  SZN  dicirikan   sebagai  enzim  termostabil dengan  aktiviti  optimum  pada  60°C,  pH  7.5,  tempoh  separuh   hayat  16  jam  dengan peningkatan  aktiviti  dan  kestabilan  dalam  beberapa  ion  logam  dan   perencat  yang diuji.  Keputusan  daripada  penentuan  struktur  menunjukkan   pengurangan    a-heliks secara   beransur-ansur   daripada   36   hingga   27.6%,   diikuti   dengan    disorientasi mendadak   struktur  tersebut  pada  peralihan   suhu  daripada  60  hingga  70°C   (27.6 hingga 19.5%). Sebaliknya, peratusan  lembaran   ,  telah meningkat  secara stabil pada suhu  yang diuji. Struktur a-heliks yang juga merupakan  lokasi residu-residu  pengikat logam dan  pemangkin  yang penting, lemah sepenuhnya pada suhu melebihi 70°C lalu mengakibatkan  kehilangan  aktiviti. Kesimpulannya, aminoasilase  SZN telah dicirikan sebagai   enzim   termostabil    berdasarkan   kepada   integriti   struktur   a-heliks   dan kestabilan  fungsinya  pada  suhu   tinggi.  Implikasi  kajian  ini  menunjukkan   bahawa aminoasilase  SZN  mampu  menjadi  enzim   altematif  kepada  bioindustri  berdasarkan kepada  peningkatan  aktiviti  enzim  dalam  suhu   tinggi  dan  kestabilan  dalam  pelbagai perencat yang diuji.  

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