Antifungal activity and phytochemical screening of vernonia amygdalina extract against botrytis cinerea causing gray mold disease on tomato fruits

Siti Fairuz Yusoff

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Type :	article
Subject :	S Agriculture (General)
Main Author :	Siti Fairuz Yusoff
Additional Authors :	Farah Farhanah Haron Mahmud Tengku Muda Mohamed Norhayu Asib Siti Zaharah Sakimin Faizah Abu Kassim Siti Izera Ismail
Title :	Antifungal activity and phytochemical screening of vernonia amygdalina extract against botrytis cinerea causing gray mold disease on tomato fruits

Place of Production :	Tanjong Malim
Publisher :	Fakulti Teknikal dan Vokasional
Year of Publication :	2019
Corporate Name :	Universiti Pendidikan Sultan Idris

Abstract : Universiti Pendidikan Sultan Idris

Gray mold disease caused by Botrytis cinerea is a damaging postharvest disease in tomato plants, and it is known to be a limiting factor in tomato production. This study aimed to evaluate antifungal activities of Vernonia amygdalina leaf extracts against B. cinerea and to screen the phytochemical compound in the crude extract that had the highest antifungal activity. In this study, crude extracts of hexane, dichloromethane, methanol, and water extracts with concentration levels at 100, 200, 300, 400, and 500 mg/mL were shown to significantly affect the inhibition of B. cinerea. Among the crude extracts, dichloromethane extract was shown to be the most potent in terms of antifungal activities. The SEM observation proved that the treatment altered the fungal morphology, which leads to fungal growth inhibition. For the in vivo bioassay, the fruits treated with dichloromethane extract at 400 and 500 mg/mL showed the lowest disease incidence with mild severity of infection. There were 23 chemical compounds identified in V. amygdalina dichloromethane extract using GCMS analysis. The top five major compounds were dominated by squalene (16.92%), phytol (15.05%), triacontane (11.31%), heptacosane (7.14%), and neophytadiene (6.28%). Some of these significant compounds possess high antifungal activities. This study proved that V. amygdalina from dichloromethane extract could be useful for inhibiting gray mold disease on tomato fruit and has potential as a natural antifungal agent.

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