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Type :thesis
Subject :QH Natural history
Main Author :Ateshan,Haidr Msahir
Title :Evaluation of habitat quality and allergenic characterization of mud crab (Scylla olivacea) from Sungai Merbok Kedah
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2021
Corporate Name :Universiti Pendidikan Sultan Idris
PDF Guest :Click to view PDF file
PDF Full Text :The author has requested the full text of this item to be restricted.

Abstract : Universiti Pendidikan Sultan Idris
This study aims to evaluate the habitat quality and allergenic characterization of mud crab, Scylla olivacea from Merbok River, Kedah. The habitat quality variables, biological contaminants and levels of heavy metals in both water and S. olivacea were analyzed using Ion chromatography, Coupled Plasma Mass Spectrometry (ICP-MS) and biological standard tests. Meanwhile, the allergenic characteristics of this crab were identified by proteomics methods including SDS-PAGE, immunoblotting, 2-DE and mass spectrometry analysis. The correlation of habitat quality and the crab allergenicity was determined by bivariate correlation. Most of the habitat quality parameters, biological contaminants and level of heavy metal present in concentrations exceeded the reference standard limits in both river water and S. olivacea, indicating pollution of the river water. The raw mix crab extracts have 26 to 33 protein bands, and 21 of them were allergenic. Four major allergens of S. olivacea were identified. The allergens of 20, 36, 43 and 75 kDa were identified as actin, tropomyosin, arginine kinase and hemocyanin, respectively. A majority of the allergenic proteins were detected in all crab parts but at various frequencies. Some of these major and minor allergens were stable to boiling and acid treatments. Strong positive correlations between Cr-Zn, Cr-As, Cr-Pb, Mn-As, Mn-Pb, Fe-Ni, Zn-As and Zn-Pb. It should be noted that Cr, As, Pb and Fe are strongly negatively correlated with total protein concentration and number of protein bands in raw mix parts of Scylla olivacea, suggesting that a relationship exists between habitat quality and the protein profiles of S. olivacea. This study could facilitate the improvement of crab allergic patients’ management as high contamination from the surrounding environment of S. olivacea might result in health risks to consumers including allergic patients.

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