UPSI Digital Repository (UDRep)
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Abstract : Universiti Pendidikan Sultan Idris |
This study aims to evaluate the habitat quality and allergenic characterization of mud crab,
Scylla olivacea from Merbok River, Kedah. The habitat quality variables, biological
contaminants and levels of heavy metals in both water and S. olivacea were analyzed using
Ion chromatography, Coupled Plasma Mass Spectrometry (ICP-MS) and biological
standard tests. Meanwhile, the allergenic characteristics of this crab were identified by
proteomics methods including SDS-PAGE, immunoblotting, 2-DE and mass spectrometry
analysis. The correlation of habitat quality and the crab allergenicity was determined by
bivariate correlation. Most of the habitat quality parameters, biological contaminants and
level of heavy metal present in concentrations exceeded the reference standard limits in
both river water and S. olivacea, indicating pollution of the river water. The raw mix crab
extracts have 26 to 33 protein bands, and 21 of them were allergenic. Four major allergens
of S. olivacea were identified. The allergens of 20, 36, 43 and 75 kDa were identified as
actin, tropomyosin, arginine kinase and hemocyanin, respectively. A majority of the
allergenic proteins were detected in all crab parts but at various frequencies. Some of these
major and minor allergens were stable to boiling and acid treatments. Strong positive
correlations between Cr-Zn, Cr-As, Cr-Pb, Mn-As, Mn-Pb, Fe-Ni, Zn-As and Zn-Pb. It
should be noted that Cr, As, Pb and Fe are strongly negatively correlated with total protein
concentration and number of protein bands in raw mix parts of Scylla olivacea, suggesting
that a relationship exists between habitat quality and the protein profiles of S. olivacea.
This study could facilitate the improvement of crab allergic patients’ management as high
contamination from the surrounding environment of S. olivacea might result in health risks
to consumers including allergic patients. |
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