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Type :thesis
Subject :QH Natural history
Main Author :Thamer, Dalfi Mazin Maky
Title :Molecular characterization of interleukin-4 and interleukin-6 in patients with type-2 diabetes mellitus in Iraq
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2021
Corporate Name :Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris
This study aimed to investigate the association of polymorphisms of Interleukin-4 (IL- 4) and Interleukin-6 (IL-6) genes to the susceptibility and severity of type-2 diabetes mellitus (T2DM) and to evaluate the serum levels of these interleukins in patients with T2DM. A number of 90 individuals from Wasit province, Iraq inclusive of 64 patients with T2DM and 26 healthy individuals with normal fasting blood sugar as a control group. Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of IL-4 and IL-6 in sera from patients with T2DM. The allele-specific polymerase chain reaction (PCR) technique was used to investigate the polymorphism of the interleukins: IL4 -590C/T, (rs2243250) and IL6 -174G/C, (rs1800795) genes polymorphisms. Results of this study showed that IL-4 concentrations had a non-significant difference (p = >0.052) between patients with T2DM (154.48 pg/ml ± 7.00) with the control group (151.49 pg/ml ± 6.21). Patients with T2DM revealed an elevated serum level of IL-6 (637.1 pg/ml ± 355.9) compared to control group (266.3 pg/ml ± 128.8). IL4 −590 C/T displayed that C allele is a major allele in the studied group. The CC genotype has an association with T2DM and this genotype increases the probability of contracting the T2DM with an odds ratio (OR) = 1.2222. The genetic polymorphism of IL6 −174G/C patients with T2DM showed a significant frequency (chi-square = 0.601, p = 0.001) of IL-6 G/C genotype (31.25%) in comparison with controls (23%). IL6 −174G/C displayed that G allele is a major allele in the studied group. As a conclusion, the levels of IL-4 may not be associated with T2DM, whereas, the elevated levels of IL-6 may be related with T2DM. The study implicates that functional gene polymorphisms of IL-4 and IL-6 will provide additional information related to the pathogenesis of diabetes mellitus.

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