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Abstract : Universiti Pendidikan Sultan Idris |
Type-2 diabetes mellitus is the most common chronic metabolic disorder characterized by elevation of blood glucose concentration (hyperglycemia) due to defect in carbohydrate, lipid and protein metabolism. Cytokines have an important role in impairing insulin signaling and selective destruction of insulin-producing beta cells. Thus, the second type of diabetes can be characterized as an immune-mediated disease. This study aimed to evaluate the serum levels of Interleukin-4 (IL-4) and Interleukin-6 (IL-6) of patients with type 2 diabetes mellitus among cases from Wasit province-Iraq. A total of 90 randomly selected subjects from Wasit province ? Iraq: (60) patients with T2DM, and (30) apparently healthy subjects with normal fasting blood sugar as a control group. Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of interleukin-4 IL-4 and interleukin-6 in sera from patients with type-2 diabetes mellitus. The results of this study showed that IL-4 concentrations had a non-significant difference when compared patients with type-2 diabetes mellitus with the control group (154 � 7.00 versus (vs) 151.49 � 21, P-value (P) = > 0.05). While patients with T2DM revealed elevated serum levels of IL-6 compared to control group (B 637.1 � 355.9 versus 266.3 � 128.8, P = < 0.001). ? 2021, Institute of Medico-Legal Publications. All rights reserved. |
References |
Abbatecola, A. M., Ferrucci, L., Grella, R., Bandinelli, S., Bonafè, M., Barbieri, M., . . . Paolisso, G. (2004). Diverse effect of inflammatory markers on insulin resistance and insulin-resistance syndrome in the elderly. Journal of the American Geriatrics Society, 52(3), 399-404. doi:10.1111/j.1532-5415.2004.52112.x Acharya, A. B., Thakur, S., Muddapur, M. V., & Kulkarni, R. D. (2016). Tumor necrosis factor-α, interleukin-4 and -6 in the serum of health, chronic periodontitis, and type 2 diabetes mellitus. Journal of Indian Society of Periodontology, 20(5), 509-513. doi:10.4103/0972-124X.201694 Al-Goblan, A. S., Al-Alfi, M. A., & Khan, M. Z. (2014). Mechanism linking diabetes mellitus and obesity. Diabetes, Metabolic Syndrome and Obesity, 7, 587-591. doi:10.2147/DMSO.S67400 Ates, I. (2018). Type 2 diabetes mechanisms, role of cytokines and their variations in disease development. Fabad Journal of Pharmaceutical Sciences, 43(1), 31-40. Retrieved from www.scopus.com Banerjee, M., & Saxena, M. (2014). Genetic polymorphisms of cytokine genes in type 2 diabetes mellitus. World J Diabetes, 5(4), 493-504. Retrieved from www.scopus.com Cieślak, M., Wojtczak, A., & Cieślak, M. (2015). Role of pro-inflammatory cytokines of pancreatic islets and prospects of elaboration of new methods for the diabetes treatment. Acta Biochimica Polonica, 62(1), 15-21. doi:10.18388/abp.2014_853 Elbe-Bürger, A., Egyed, A., Olt, S., Klubal, R., Mann, U., Rappersberger, K., . . . Stingl, G. (2002). Overexpression of IL-4 alters the homeostasis in the skin. Journal of Investigative Dermatology, 118(5), 767-778. doi:10.1046/j.1523-1747.2002.01753.x Erbaǧci, A. B., Tarakçioǧlu, M., Coşkun, Y., Sivasli, E., & Sibel Namiduru, E. (2001). Mediators of inflammation in children with type I diabetes mellitus: Cytokines in type I diabetic children. Clinical Biochemistry, 34(8), 645-650. doi:10.1016/S0009-9120(01)00275-2 Festa, A., D'Agostino Jr., R., Tracy, R. P., & Haffner, S. M. (2002). Elevated levels of acute-phase proteins and plasminogen activator inhibitor-1 predict the development of type 2 diabetes: The insulin resistance atherosclerosis study. Diabetes, 51(4), 1131-1137. doi:10.2337/diabetes.51.4.1131 Galavi, H. R., Saravani, R., Alamdari, A. R., Ranjbar, N., Damani, E., & Khodakhier, T. N. (2016). Evaluating the effect of the rs2229238 and the rs4845625 interleukin 6 receptor gene polymorphisms on body mass index and the risk of type 2 diabetes in an iranian study population. International Journal of High Risk Behaviors and Addiction, 5(4) doi:10.5812/ijhrba.33289 Geerlings, S. E., Brouwer, E. C., Van Kessel, K. C. P. M., Gaastra, W., Stolk, R. P., & Hoepelman, A. I. M. (2000). Cytokine secretion is impaired in women with diabetes mellitus. European Journal of Clinical Investigation, 30(11), 995-1001. doi:10.1046/j.1365-2362.2000.00745.x Gomes, K. B. (2017). IL-6 and type 1 diabetes mellitus: T cell responses and increase in IL-6 receptor surface expression. Annals of Translational Medicine, 5(1) doi:10.21037/atm.2016.12.74 Groop, L. C., Saloranta, C., Shank, M., Bonadonnaj, R. C., Ferrannini, E., & Defronzo, R. A. (1991). The role of free fatty acid metabolism in the pathogenesis of insulin resistance in obesity and noninsulin-dependent diabetes mellitus. Journal of Clinical Endocrinology and Metabolism, 72(1), 96-107. doi:10.1210/jcem-72-1-96 Hak, A. E., Pols, H. A. P., Stehouwer, C. D. A., Meijer, J., Kiliaan, A. J., Hofman, A., . . . Witteman, J. C. M. (2001). Markers of inflammation and cellular adhesion molecules in relation to insulin resistance in nondiabetic elderly: The rotterdam study. Journal of Clinical Endocrinology and Metabolism, 86(9), 4398-4405. doi:10.1210/jcem.86.9.7873 Hashizume, M., & Mihara, M. (2011). IL-6 and lipid metabolism. Inflamm Regen, 31(3), 325-333. Retrieved from www.scopus.com Helaly, M. A. -., Hatata, E. -. Z., Abu-Elmagd, M., Ibrahem, E. F., Alsaid, A., El-Aal, I. A. A., & Settin, A. (2013). Association of IL-10 and IL-6 gene polymorphisms with type 2 diabetes mellitus among egyptian patients. [Mısır'lı hastalarda tip 2 diyabet ile IL-10 ve IL-6 gen poliformizmi arasındaki i̇lişki] European Journal of General Medicine, 10(3), 158-162. doi:10.29333/ejgm/82250 Hotamisligil, G. S., Arner, P., Caro, J. F., Atkinson, R. L., & Spiegelman, B. M. (1995). Increased adipose tissue expression of tumor necrosis factor-α in human obesity and insulin resistance. Journal of Clinical Investigation, 95(5), 2409-2415. doi:10.1172/JCI117936 Hotamisligil, G. S., Peraldi, P., Budavari, A., Ellis, R., White, M. F., & Spiegelman, B. M. (1996). IRS-1-mediated inhibition of insulin receptor tyrosine kinase activity in TNF-α- and obesity-induced insulin resistance. Science, 271(5249), 665-668. doi:10.1126/science.271.5249.665 Hussain, M. K., Deli, F. A., Algenabi, A. H. A., & Abdul-Rudha, K. H. (2018). Adiponectin gene polymorphisms as a predictor for development of type 2 diabetes mellitus in iraqi population. Gene, 662, 118-122. doi:10.1016/j.gene.2018.03.087 Jain, S. K., Kannan, K., Lim, G., Matthews-Greek, J., McVie, R., & Bocchini Jr., J. A. (2003). Elevated blood interleukin-6 levels in hyperketonemic type 1 diabetic patients and secretion by acetoacetate-treated cultured U937 monocytes. Diabetes Care, 26(7), 2139-2143. doi:10.2337/diacare.26.7.2139 Kiani, A., García-Cózar, F. J., Habermann, I., Laforsch, S., Aebischer, T., Ehninger, G., & Rao, A. (2001). Regulation of interferon-γ gene expression by nuclear factor of activated T cells. Blood, 98(5), 1480-1488. doi:10.1182/blood.V98.5.1480 Kieffer, T. J., Heller, R. S., Leech, C. A., Holz, G. G., & Habener, J. F. (1997). Leptin suppression of insulin secretion by the activation of ATP- sensitive K+ channels in pancreatic β-cells. Diabetes, 46(6), 1087-1093. doi:10.2337/diab.46.6.1087 Kristiansen, O. P., & Mandrup-Poulsen, T. (2005). Interleukin-6 and diabetes: The good, the bad, or the indifferent? Diabetes, 54(SUPPL. 2), S114-S124. doi:10.2337/diabetes.54.suppl_2.S114 Kulseng, B., Skjåk-BræK, G., Følling, I., & Espevik, T. (1996). TNF production from peripheral blood mononuclear cells in diabetic patients after stimulation with alginate and lipopolysaccharide. Scandinavian Journal of Immunology, 43(3), 335-340. doi:10.1046/j.1365-3083.1996.d01-43.x Liu, S., Tinker, L., Song, Y., Rifai, N., Bonds, D. E., Cook, N. R., . . . Manson, J. E. (2007). A prospective study of inflammatory cytokines and diabetes mellitus in a multiethnic cohort of postmenopausal women. Archives of Internal Medicine, 167(15), 1676-1685. doi:10.1001/archinte.167.15.1676 Lowe, G., Woodward, M., Hillis, G., Rumley, A., Li, Q., Harrap, S., . . . Chalmers, J. (2014). Circulating inflammatory markers and the risk of vascular complications and mortality in people with type 2 diabetes and cardiovascular disease or risk factors: The advance study. Diabetes, 63(3), 1115-1123. doi:10.2337/db12-1625 Malenica, M., Šilar, M., Dujić, T., Bego, T., Semiz, S., Škrbo, S., . . . Čaušević, A. (2017). Importance of inflammatory markers and IL-6 for diagnosis and follow up of patients with type 2 diabetes mellitus. [Značaj inflamatornih markera i IL-6 u dijagnozi i praćenju pacijenata s dijabetes melitusom tipa 2] Medicinski Glasnik, 14(2), 169-175. doi:10.17392/920-17 Mcneely, M. J., Chessler, S. D., Boyko, E. J., Leonnetti, D. L., Weigle, D. S., Fujimoto, W. Y., & Shofer, J. B. (1999). Association between baseline plasma leptin levels and subsequent development of diabetes in japanese americans. Diabetes Care, 22(1), 65-70. doi:10.2337/diacare.22.1.65 Nuhiar, R. S., Salman, A. N., & AL-Rekaby, H. R. (2018). The some cytokines levels (TGFβ1, IL-4, IL-6, and IL-17) in sera patients with diabetes mellitus Type1, type 2 in nassiriya city. J.Thi-Qar Sci, 6(4), 42-48. Retrieved from www.scopus.com Panagi, L., Poole, L., Hackett, R. A., & Steptoe, A. (2019). Sex differences in interleukin-6 stress responses in people with type 2 diabetes. Psychophysiology, 56(6) doi:10.1111/psyp.13334 Papaetis, G. S., Papakyriakou, P., & Panagiotou, T. N. (2015). Central obesity, type 2 diabetes and insulin: Exploring a pathway full of thorns. Archives of Medical Science, 11(3), 463-482. doi:10.5114/aoms.2015.52350 Pesu, M., Takaluoma, K., Aittomäki, S., Lagerstedt, A., Saksela, K., Kovanen, P. E., & Silvennoinen, O. (2000). Interleukin-4-induced transcriptional activation by Stat6 involves multiple serine/threonine kinase pathways and serine phosphorylation of Stat6. Blood, 95(2), 494-502. doi:10.1182/blood.v95.2.494 Pradhan, A. D., Manson, J. E., Rifai, N., Buring, J. E., & Ridker, P. M. (2001). C-reactive protein, interleukin 6, and risk of developing type 2 diabetes mellitus. Journal of the American Medical Association, 286(3), 327-334. doi:10.1001/jama.286.3.327 Punthakee, Z., Goldenberg, R., Katz, P., & Diabetes Canada Clinical Practice Guidelines Expert Committee. (2018). Definition, classification and diagnosis of diabetes, prediabetes and metabolic syndrome. Canadian Journal of Diabetes, 42, S10-S15. doi:10.1016/j.jcjd.2017.10.003 Qu, D., Liu, J., Lau, C. W., & Huang, Y. (2014). IL-6 in diabetes and cardiovascular complications. British Journal of Pharmacology, 171(15), 3595-3603. doi:10.1111/bph.12713 Ribeiro, F. V., De Mendoncxa, A. C., Santos, V. R., Bastos, M. F., Figueiredo, L. C., & Duarte, P. M. (2011). Cytokines and bone-related factors in systemically healthy patients with chronic periodontitis and patients with type 2 diabetes and chronic periodontitis. Journal of Periodontology, 82(8), 1187-1196. doi:10.1902/jop.2011.100643 Rodrigues, K. F., Pietrani, N. T., Bosco, A. A., Campos, F. M. F., Sandrim, V. C., & Gomes, K. B. (2017). IL-6, TNF-α, and IL-10 levels/ polymorphisms and their association with type 2 diabetes mellitus and obesity in brazilian individuals. Archives of Endocrinology and Metabolism, 61(5), 438-446. doi:10.1590/2359-3997000000254 Saxena, M., Agrawal, C. G., Srivastava, N., & Banerjee, M. (2014). Interleukin-6 (IL-6)-597 A/G (rs1800797) & -174 G/C (rs1800795) gene polymorphisms in type 2 diabetes. Indian Journal of Medical Research, 140(JUL), 60-68. Retrieved from www.scopus.com Schultz, O., Oberhauser, F., Saech, J., Rubbert-Roth, A., Hahn, M., Krone, W., & Laudes, M. (2010). Effects of inhibition of interleukin-6 signalling on insulin sensitivity and lipoprotein (A) levels in human subjects with rheumatoid diseases. PLoS ONE, 5(12) doi:10.1371/journal.pone.0014328 Shih, Y. L., Ho, K. T., Tsao, C. H., Chang, Y. H., Shiau, M. Y., & Huang, C. N. (2013). Role of cyotkines in metabolism and type 2 diabetes mellitus. Int J Biomed Lab Sci, 2(1), 1-6. Retrieved from www.scopus.com Spranger, J., Kroke, A., Möhlig, M., Hoffmann, K., Bergmann, M. M., Ristow, M., . . . Pfeiffer, A. F. H. (2003). Inflammatory cytokines and the risk to develop type 2 diabetes: Results of the prospective population-based european prospective investigation into cancer and nutrition (EPIC)-potsdam study. Diabetes, 52(3), 812-817. doi:10.2337/diabetes.52.3.812 Steppan, C. M., Bailey, S. T., Bhat, S., Brown, E. J., Banerjee, R. R., Wright, C. M., . . . Lazar, M. A. (2001). The hormone resistin links obesity to diabetes. Nature, 409(6818), 307-312. doi:10.1038/35053000 Tavangar, A., Khozeimeh, F., Ghoreishian, F., & Boroujeni, M. A. (2016). Serum level of interleukin-8 in subjects with diabetes, diabetes plus oral lichen planus, and oral lichen planus: A biochemical study. Dental Research Journal, 13(5), 413-418. doi:10.4103/1735-3327.192277 Vidhate, D. A., Thomas, J., & Gupte, A. M. (2013). Association of IL-6 with diabetes mellitus in indian population from navi mumbai. International Journal of Recent Trends in Science and Technology, 8(2), 100-102. Retrieved from www.scopus.com Wȩdrychowicz, A., Dziatkowiak, H., Sztefko, K., & Wȩdrychowicz, A. (2004). Interleukin-6 (IL-6) and IGF-IGFBP system in children and adolescents with type 1 diabetes mellitus. Experimental and Clinical Endocrinology and Diabetes, 112(8), 435-439. doi:10.1055/s-2004-821189 Wei, H., Feng, G., Pei, W., Gang, W., & Yuelin, Z. (2017). Association between interleukin-4 genetic polymorphisms and the risk of cerebral infarction in a population of china. Biomedical Research (India), 28(21), 9198-9203. Retrieved from www.scopus.com |
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