UPSI Digital Repository (UDRep)
|
|
|
Abstract : Universiti Pendidikan Sultan Idris |
Background: Tropomyosin is a major allergen in crustaceans, including mud crab species, but its molecular and allergenic properties in Scylla olivacea are not well known. Thus, this study aimed to produce the recombinant tropomyosin protein from S. olivacea and subsequently investigate its IgE reactivity. Methods and Results: The tropomyosin gene was cloned and expressed in the Escherichia coli system, followed by SDS-PAGE and immunoblotting test to identify the allergenic potential of the recombinant protein. The 855-base pair of tropomyosin gene produced was found to be 99.18% homologous to Scylla serrata. Its 284 amino acids matched the tropomyosin of crustaceans, arachnids, insects, and Klebsiella pneumoniae, ranging from 79.03 to 95.77%. The tropomyosin contained 89.44% alpha-helix folding with a tertiary structure of two-chain alpha-helical coiled-coil structures comprising a homodimer heptad chain. IPTG-induced histidine tagged-recombinant tropomyosin was purified at the size of 42�kDa and confirmed as tropomyosin using anti-tropomyosin monoclonal antibodies. The IgE binding of recombinant tropomyosin protein was reactive in 90.9% (20/22) of the sera from crab-allergic patients. Conclusions: This study has successfully produced an allergenic recombinant tropomyosin from S. olivacea. This recombinant tropomyosin may be used as a specific allergen for the diagnosis of allergy. ? 2021, The Author(s), under exclusive licence to Springer Nature B.V. |
References |
Aalberse, R. C. (2000). Structural biology of allergens. Journal of Allergy and Clinical Immunology, 106(2), 228-238. doi:10.1067/mai.2000.108434 Abramovitch, J. B., Kamath, S., Varese, N., Zubrinich, C., Lopata, A. L., O'Hehir, R. E., & Rolland, J. M. (2013). IgE reactivity of blue swimmer crab (portunus pelagicus) tropomyosin, por p 1, and other allergens; cross-reactivity with black tiger prawn and effects of heating. PLoS ONE, 8(6) doi:10.1371/journal.pone.0067487 Ansotegui, I. J., Melioli, G., Canonica, G. W., Caraballo, L., Villa, E., Ebisawa, M., . . . Zuberbier, T. (2020). IgE allergy diagnostics and other relevant tests in allergy, a world allergy organization position paper. World Allergy Organization Journal, 13(2) doi:10.1016/j.waojou.2019.100080 Ar, N. I., Rosmilah, M., My, Z. H., Noormalin, A., Faizal, B., & Shahnaz, M. (2015). Identification of major and minor allergens of mud crab (scylla serrata). Med Health, 10(2), 90-97. Retrieved from www.scopus.com Asnoussi, A., Aibinu, I. E., Gasser, R. B., Lopata, A. L., & Smooker, P. M. (2017). Molecular and immunological characterisation of tropomyosin from anisakis pegreffii. Parasitology Research, 116(12), 3291-3301. doi:10.1007/s00436-017-5642-4 Asturias, J. A., Gómez-Bayón, N., Arilla, M. C., Martínez, A., Palacios, R., Sánchez-Gascón, F., & Martínez, J. (1999). Molecular characterization of american cockroach tropomyosin (periplaneta americana allergen 7), a cross-reactive allergen. Journal of Immunology, 162(7), 4342-4348. Retrieved from www.scopus.com Ayuso, R., Lehrer, S. B., & Reese, G. (2002). Identification of continuous, allergenic regions of the major shrimp allergen pen a 1 (tropomyosin). International Archives of Allergy and Immunology, 127(1), 27-37. doi:10.1159/000048166 Ayuso, R., Reese, G., Leong-Kee, S., Plante, M., & Lehrer, S. B. (2002). Molecular basis of arthropod cross-reactivity: Ige-binding cross-reactive epitopes of shrimp, house dust mite and cockroach tropomyosins. International Archives of Allergy and Immunology, 129(1), 38-48. doi:10.1159/000065172 Azemi, N. F. H., Misnan, R., Keong, P. B., & Yadzir, Z. H. M. (2020). Reference gene and tropomyosin expression in mud crab scylla olivacea, scylla paramamosain and scylla tranquebarica. Molecular Biology Reports, 47(12), 9765-9777. doi:10.1007/s11033-020-05966-7 Azra, M. N., & Ikhwanuddin, M. (2016). A review of maturation diets for mud crab genus scylla broodstock: Present research, problems and future perspective. Saudi Journal of Biological Sciences, 23(2), 257-267. doi:10.1016/j.sjbs.2015.03.011 Bird, J. A., Lack, G., & Perry, T. T. (2015). Clinical management of food allergy. Journal of Allergy and Clinical Immunology: In Practice, 3(1), 1-11. doi:10.1016/j.jaip.2014.06.008 Chapman, M. D., Smith, A. M., Vailes, L. D., Arruda, L. K., Dhanaraj, V., & Pomés, A. (2000). Recombinant allergens for diagnosis and therapy of allergic disease. Journal of Allergy and Clinical Immunology, 106(3), 409-418. doi:10.1067/mai.2000.109832 Cui, Y., Zhou, Y., Wang, Y., Ma, G., & Yang, L. (2013). The group 10 allergen of dermatophagoides farinae (acari: Pyroglyphidae): CDNA cloning, sequence analysis, and expression in escherichia coli BL21. Journal of Medical Entomology, 50(1), 205-208. doi:10.1603/ME12019 Devi, P. L., & Joseph, A. (2015). Taxonomy of mud crabs of the genus scylla (de haan) from cochin backwaters, kerala india. Indian J Fish, 62(3), 45-51. Retrieved from www.scopus.com Faisal, M., Vasiljevic, T., & Donkor, O. N. (2019). Effects of selected processing treatments on antigenicity of banana prawn (fenneropenaeus merguiensis) tropomyosin. International Journal of Food Science and Technology, 54(1), 183-193. doi:10.1111/ijfs.13922 Fazhan, H., Waiho, K., Quinitio, E., Baylon, J. C., Fujaya, Y., Rukminasari, N., . . . Ikhwanuddin, M. (2020). Morphological descriptions and morphometric discriminant function analysis reveal an additional four groups of scylla spp. PeerJ, 2020(1) doi:10.7717/peerj.8066 Galli, S. J., & Tsai, M. (2012). IgE and mast cells in allergic disease. Nature Medicine, 18(5), 693-704. doi:10.1038/nm.2755 González-Fernández, J., Rodero, M., Daschner, A., & Cuéllar, C. (2014). New insights into the allergenicity of tropomyosin: A bioinformatics approach. Molecular Biology Reports, 41(10), 6509-6517. doi:10.1007/s11033-014-3534-6 Ikhwanuddin, M., Muhd-Farouk, H., Memon, A. J., Wendy, W., & Abol-Munafi, A. B. (2014). Sperm viability assessment over elapsing time maintained at 2°c of orange mud crab, scylla olivacea (herbst, 1796). Pakistan Journal of Biological Sciences, 17(9), 1069-1073. doi:10.3923/pjbs.2014.1069.1073 James, J. K., Pike, D. H., Khan, I. J., & Nanda, V. (2018). Structural and dynamic properties of allergen and non-allergen forms of tropomyosin. Structure, 26(7), 997-1006.e5. doi:10.1016/j.str.2018.05.002 Jasim, H. A., Misnan, R., Yadzir, Z. H. M., Abdullah, N., Bakhtiar, F., Arip, M., . . . Keong, P. B. (2021). Identification of common and novel major crab allergens in scylla tranquebarica and the allergen stability in untreated and vinegar-treated crab. Iranian Journal of Allergy, Asthma, and Immunology, 20(1), 76-87. doi:10.18502/ijaai.v20i1.5414 Jeebhay, M. F., Robins, T. G., Seixas, N., Baatjies, R., George, D. A., Rusford, E., . . . Lopata, A. L. (2005). Environmental exposure characterization of fish processing workers. Annals of Occupational Hygiene, 49(5), 423-437. doi:10.1093/annhyg/meh113 Jeong, K. Y. (2004). Characterization of allergenic properties of german cockroach tropomyosin using recombinant proteins. Anim Cells Syst, 8, 73. Retrieved from www.scopus.com Jeong, K. Y., Lee, J., Lee, I. -., Ree, H. -., Hong, C. -., & Yong, T. -. (2003). Allergenicity of recombinant bla g 7, german cockroach tropomyosin. Allergy: European Journal of Allergy and Clinical Immunology, 58(10), 1059-1063. doi:10.1034/j.1398-9995.2003.00167.x Jeong, K. Y., Yum, H. -., Lee, I. -., Ree, H. -., Hong, C. -., Kim, D. S., & Yong, T. -. (2004). Molecular cloning and characterization of tropomyosin, a major allergen of chironomus kiiensis, a dominant species of nonbiting midges in korea. Clinical and Diagnostic Laboratory Immunology, 11(2), 320-324. doi:10.1128/CDLI.11.2.320-324.2004 Kamath, S. D., Rahman, A. M. A., Komoda, T., & Lopata, A. L. (2013). Impact of heat processing on the detection of the major shellfish allergen tropomyosin in crustaceans and molluscs using specific monoclonal antibodies. Food Chemistry, 141(4), 4031-4039. doi:10.1016/j.foodchem.2013.06.105 Keenan, C. P., Davie, P. J. F., & Mann, D. L. (1998). A revision of the genus scylla de haan, 1833 (crustacea: Decapoda: Brachyura: Portunidae). Raffles Bulletin of Zoology, 46(1), 217-245. Retrieved from www.scopus.com Koeberl, M., Kamath, S. D., Saptarshi, S. R., Smout, M. J., Rolland, J. M., O'Hehir, R. E., & Lopata, A. L. (2014). Auto-induction for high yield expression of recombinant novel isoallergen tropomyosin from king prawn (melicertus latisulcatus) for improved diagnostics and immunotherapeutics. Journal of Immunological Methods, 415, 6-16. doi:10.1016/j.jim.2014.10.008 Krieger, J., Raming, K., Knipper, M., Grau, M., Mertens, S., & Breer, H. (1990). Cloning, sequencing and expression of locust tropomyosin. Insect Biochemistry, 20(2), 173-184. doi:10.1016/0020-1790(90)90010-R Kumjim, S., Jirapongsananuruk, O., & Piboonpocanun, S. (2016). Cloning and characterization of recombinant tropomyosin of giant freshwater shrimp M. rosenbergii to determine major allergens causing allergic reactions among shrimp-allergic children. Asian Pacific Journal of Allergy and Immunology, 34(3), 229-235. doi:10.12932/AP0698 Kyoung, Y. J., Lee, H., Jae, S. L., Lee, J., Lee, I. -., Ree, H. -., . . . Yong, T. -. (2007). Molecular cloning and the allergenic characterization of tropomyosin from tyrophagus putrescentiae. Protein and Peptide Letters, 14(5), 431-436. doi:10.2174/092986607780782777 Larsen, J. M., Bang-Berthelsen, C. H., Qvortrup, K., Sancho, A. I., Hansen, A. H., Andersen, K. I. H., . . . Bøgh, K. L. (2020). Production of allergen-specific immunotherapeutic agents for the treatment of food allergy. Critical Reviews in Biotechnology, 40(6), 881-894. doi:10.1080/07388551.2020.1772194 Leung, P. S. C., Chen, Y. -., Gershwin, M. E., Wong, S. H., Kwan, H. S., & Chu, K. H. (1998). Identification and molecular characterization of charybdis feriatus tropomyosin, the major crab allergen. Journal of Allergy and Clinical Immunology, 102(5), 847-852. doi:10.1016/S0091-6749(98)70027-2 Leung, P. S. C., Wing Kuen Chow, Duffey, S., Hoi Shan Kwan, Gershwin, M. E., & Ka Hou Chu. (1996). IgE reactivity against a cross-reactivity allergen in crustacea and mollusca: Evidence for tropomyosin as the common allergen. Journal of Allergy and Clinical Immunology, 98(5), 954-961. doi:10.1016/S0091-6749(96)80012-1 Liang, Y. -., Cao, M. -., Su, W. -., Zhang, L. -., Huang, Y. -., & Liu, G. -. (2008). Identification and characterisation of the major allergen of chinese mitten crab (eriocheir sinensis). Food Chemistry, 111(4), 998-1003. doi:10.1016/j.foodchem.2008.05.023 Liu, G. -., Cao, M. -., Huang, Y. -., Cai, Q. -., Weng, W. -., & Su, W. -. (2010). Comparative study of in vitro digestibility of major allergen tropomyosin and other food proteins of chinese mitten crab (eriocheir sinensis). Journal of the Science of Food and Agriculture, 90(10), 1614-1620. doi:10.1002/jsfa.3988 Lopata, A. L., & Kamath, S. (2012). Shellfish allergy diagnosis - gaps and needs. Current Allergy and Clinical Immunology, 25(2), 60-66. Retrieved from www.scopus.com Misnan, R., Abdul Rahman, N. I., & Yadzir, Z. H. M. (2015). Characterization of major allergens of local mud crab (scylla serrata). Sci Res J, 12(1), 1-10. Retrieved from www.scopus.com Misnan, R., Murad, S., Yadzir, Z. H. M., & Abdullah, N. (2012). Identification of the major allergens of charybdis feriatus (red crab) and its cross-reactivity with portunus pelagicus (blue crab). Asian Pacific Journal of Allergy and Immunology, 30(4), 285-293. Retrieved from www.scopus.com Nasrat, M., Misnan, R., & Yadzir, Z. H. M. (2018). Comparison of protein profiles and allergenicity of different body parts and genders of scylla paramamosain. Int J Sci Environ Technol, 7(5), 1759-1765. Retrieved from www.scopus.com Pekar, J., Ret, D., & Untersmayr, E. (2018). Stability of allergens. Molecular Immunology, 100, 14-20. doi:10.1016/j.molimm.2018.03.017 Piboonpocanun, S., Malainual, N., Jirapongsananuruk, O., Vichyanond, P., & Thomas, W. R. (2006). Genetic polymorphisms of major house dust mite allergens. Clinical and Experimental Allergy, 36(4), 510-516. doi:10.1111/j.1365-2222.2006.02464.x Rahman, A. M. A., Lopata, A. L., O'Hehir, R. E., Robinson, J. J., Banoubd, J. H., & Helleur, R. J. (2010). Characterization and de novo sequencing of snow crab tropomyosin enzymatic peptides by both electrospary ionization and matrix-assisted laser desorption ionization QqToF tandemmass spectrometry. Journal of Mass Spectrometry, 45(4), 372-381. doi:10.1002/jms.1721 Rolland, J. M., Varese, N. P., Abramovitch, J. B., Anania, J., Nugraha, R., Kamath, S., . . . O'Hehir, R. E. (2018). Effect of heat processing on IgE reactivity and cross-reactivity of tropomyosin and other allergens of asia-pacific mollusc species: Identification of novel sydney rock oyster tropomyosin sac g 1. Molecular Nutrition and Food Research, 62(14) doi:10.1002/mnfr.201800148 Rosmilah, M., Shahnaz, M., Zailatul, H. M. Y., Noormalin, A., & Normilah, I. (2012). Identification of tropomyosin and arginine kinase as major allergens of portunus pelagicus (blue swimming crab). Tropical Biomedicine, 29(3), 467-478. Retrieved from www.scopus.com Shafique, R. H., Inam, M., Ismail, M., & Chaudhary, F. R. (2012). Group 10 allergens (tropomyosins) from house-dust mites may cause covariation of sensitization to allergens from other invertebrates. Allergy Rhinol (Providence), 3(2), e74-e90. Retrieved from www.scopus.com Sherson, D., Hansen, I., & Sigsgaard, T. (1989). Occupationally related respiratory symptoms in trout‐processing workers. Allergy, 44(5), 336-341. doi:10.1111/j.1398-9995.1989.tb00455.x Verhoeckx, K. C. M., Vissers, Y. M., Baumert, J. L., Faludi, R., Feys, M., Flanagan, S., . . . Kimber, I. (2015). Food processing and allergenicity. Food and Chemical Toxicology, 80, 223-240. doi:10.1016/j.fct.2015.03.005 Waiho, K., Mustaqim, M., Fazhan, H., Wan Norfaizza, W. I., Megat, F. H., & Ikhwanuddin, M. (2015). Mating behaviour of the orange mud crab, scylla olivacea: The effect of sex ratio and stocking density on mating success. Aquaculture Reports, 2, 50-57. doi:10.1016/j.aqrep.2015.08.004 Wang, X., Li, L., Xu, F., & Zhang, G. (2011). Tropomyosin is a nice marker gene for phylogenetic analysis of molluscs. Molecular Biology Reports, 38(7), 4589-4593. doi:10.1007/s11033-010-0591-3 Zailatul, H. M. Y., Leecyous, B., & Amelia Suhana, Z. (2020). Isolation and cloning of tropomyosin and arginine kinase from tiger prawn penaeus monodon and blue swimming crab portunus trituberculatus. J Sci Math Lett, 8(2), 2019-2021. Retrieved from www.scopus.com Zhang, Y., Matsuo, H., & Morita, E. (2006). Cross-reactivity among shrimp, crab and scallops in a patient with a seafood allergy. Journal of Dermatology, 33(3), 174-177. doi:10.1111/j.1346-8138.2006.00040.x |
This material may be protected under Copyright Act which governs the making of photocopies or reproductions of copyrighted materials. You may use the digitized material for private study, scholarship, or research. |