UPSI Digital Repository (UDRep)
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Abstract : Universiti Pendidikan Sultan Idris |
Kajian ini bertujuan untuk mengkaji aktiviti biologi Sargassum ilicifolium (Turner) C.
Agardh serta potensinya sebagai hormon penggalak pertumbuhan pokok cili. Aktiviti
biologi dikenal pasti melalui kehadiran bahan bioaktif menggunakan ekstrak S.
ilicifolium dalam pelarut heksana, metanol, diklorometana dan air. Ujian toksisiti
dilakukan menggunakan kaedah Brine Shrimp Lethality (BSL) terhadap udang brin
(Artemia salina). Kajian antibakteria pula, menggunakan kaedah penyebaran cakera ke
atas enam jenis bakteria dengan kepekatan ekstrak yang berbeza. Sementara itu, S.
ilicifolium telah diekstrak menggunakan pelarut dengan kepekatan yang berbeza untuk
dijadikan sebagai hormon penggalak pertumbuhan pokok cili. Hasil kajian
menunjukkan S. ilicifolium mengandungi alkaloid, terpenoid, tanin, saponin dan
flavonoid. Ujian toksisiti menunjukkan nilai LC50 yang berbeza dalam lingkungan
bertoksik (30 – 1000 mg ml-l) untuk kesemua ekstrak mentah iaitu heksana (234.42 mg
ml-1), methanol (371.54 mg ml-1), diklorometana (549.54 mg ml -1) dan air (93.33 mg
ml-1). Ini membuktikan semua ekstrak dianggap sebagai bertoksik. Bagi hasil kajian
antibakteria pula, ekstrak air S. ilicifolium menunjukkan aktiviti perencatan tertinggi
terhadap bakteria gram negatif, Escherichia coli (12.67 ± 1.2 mm). Kepekatan yang
rendah bagi ekstrak diklorometana (0.5mg-1) memberikan percambahan biji benih cili
yang paling tinggi berbanding kawalan. Daripada sepuluh parameter yang dikaji, hasil
menunjukkan lapan parameter iaitu panjang pokok cili (24.9 sm), panjang ruas (3.6
sm), diameter buah (12.18 mm), lebar daun (11.5 sm) panjang buah (113.86 mm), berat
segar buah (7.8 g), panjang akar (15.9 sm) dan bilangan daun (30 helai) memberikan
perbezaan min yang signifikan berbanding kawalan. Kesimpulannya, ekstrak S.
ilicifolium adalah bertoksik, mengandungi sifat antibakteria dan mempunyai metabolit
sekunder yang menggalakkan pertumbuhan pokok cili. Implikasinya, S. ilicifolium
berpotensi untuk digunakan dalam pelbagai aktiviti biologi seperti penskrinan
fitokimia, pigmentasi, antibakteria, toksisiti dan sebagai hormon penggalak
pertumbuhan pokok cili. |
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