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Type :thesis
Subject :QH Natural history
Main Author :Siti Fatimah Baharudin
Title :Aktiviti biologi Sargassum Ilicifolium (Turner) C. Agardh serta potensinya sebagai hormon penggalak pertumbuhan pokok cili
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2022
Corporate Name :Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris
Kajian ini bertujuan untuk mengkaji aktiviti biologi Sargassum ilicifolium (Turner) C. Agardh serta potensinya sebagai hormon penggalak pertumbuhan pokok cili. Aktiviti biologi dikenal pasti melalui kehadiran bahan bioaktif menggunakan ekstrak S. ilicifolium dalam pelarut heksana, metanol, diklorometana dan air. Ujian toksisiti dilakukan menggunakan kaedah Brine Shrimp Lethality (BSL) terhadap udang brin (Artemia salina). Kajian antibakteria pula, menggunakan kaedah penyebaran cakera ke atas enam jenis bakteria dengan kepekatan ekstrak yang berbeza. Sementara itu, S. ilicifolium telah diekstrak menggunakan pelarut dengan kepekatan yang berbeza untuk dijadikan sebagai hormon penggalak pertumbuhan pokok cili. Hasil kajian menunjukkan S. ilicifolium mengandungi alkaloid, terpenoid, tanin, saponin dan flavonoid. Ujian toksisiti menunjukkan nilai LC50 yang berbeza dalam lingkungan bertoksik (30 – 1000 mg ml-l) untuk kesemua ekstrak mentah iaitu heksana (234.42 mg ml-1), methanol (371.54 mg ml-1), diklorometana (549.54 mg ml -1) dan air (93.33 mg ml-1). Ini membuktikan semua ekstrak dianggap sebagai bertoksik. Bagi hasil kajian antibakteria pula, ekstrak air S. ilicifolium menunjukkan aktiviti perencatan tertinggi terhadap bakteria gram negatif, Escherichia coli (12.67 ± 1.2 mm). Kepekatan yang rendah bagi ekstrak diklorometana (0.5mg-1) memberikan percambahan biji benih cili yang paling tinggi berbanding kawalan. Daripada sepuluh parameter yang dikaji, hasil menunjukkan lapan parameter iaitu panjang pokok cili (24.9 sm), panjang ruas (3.6 sm), diameter buah (12.18 mm), lebar daun (11.5 sm) panjang buah (113.86 mm), berat segar buah (7.8 g), panjang akar (15.9 sm) dan bilangan daun (30 helai) memberikan perbezaan min yang signifikan berbanding kawalan. Kesimpulannya, ekstrak S. ilicifolium adalah bertoksik, mengandungi sifat antibakteria dan mempunyai metabolit sekunder yang menggalakkan pertumbuhan pokok cili. Implikasinya, S. ilicifolium berpotensi untuk digunakan dalam pelbagai aktiviti biologi seperti penskrinan fitokimia, pigmentasi, antibakteria, toksisiti dan sebagai hormon penggalak pertumbuhan pokok cili.

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