AbstractBackground: Eurycoma longifolia (E. longifolia), Labisia pumila (L. pumila), and Orthosiphon stamineus (O. stamineus) are popular species known for their therapeutic properties. An increase in local demand for herbal products makes them susceptible to adulteration, which poses a risk to their safety and efficacy. Current identification methods, such as organoleptic, microscopic, and macroscopic analysis, need to be revised to identify plant species in highly processed herbal products due to their limited ability to detect morphological features and provide comprehensive plant taxonomy information. Methods: This research objective was to develop a simple, reliable, and accurate DNA molecular identification method based on polymerase chain reaction_restriction fragment length polymorphism (PCR_RFLP) for E. longifolia, L. pumila, and O. stamineus, used to validate the species identification for herbal products. PCR_RFLP was developed for rapid identification using restriction enzymes TaqI.....