This study, implemented DNA Barcoding which offers great potential as a reliable identification tool to classify catches in Malaysia. Overall, this study had successfully barcoded a total of 107 species, 69 genera, 36 families and 10 order of commercial Malaysian fishes. It is found that, the snappers, threadfin fishes, parrot fishes and goatfishes sampled were detected to either experience cryptic speciation or consist of unknown/undescribed species. Conventional identification to species level using morphological characters were found to be difficult for this genera especially in cases where juvenile and adult specimens exhibit variation in colouration. Using COl gene, deep genetic divergence (K2P = 6.1%) values was obtained between LLI and LL2 groups of L. lutjanus and the subsequent analysis of both COl and cyt b sequences revealed diagnostic nucleotide substitution sites exclusively to each group. Thus, at present, this study exposed that at least one lineage represents a currentl.....

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Background: Eurycoma longifolia (E. longifolia), Labisia pumila (L. pumila), and Orthosiphon stamineus (O. stamineus) are popular species known for their therapeutic properties. An increase in local demand for herbal products makes them susceptible to adulteration, which poses a risk to their safety and efficacy. Current identification methods, such as organoleptic, microscopic, and macroscopic analysis, need to be revised to identify plant species in highly processed herbal products due to their limited ability to detect morphological features and provide comprehensive plant taxonomy information. Methods: This research objective was to develop a simple, reliable, and accurate DNA molecular identification method based on polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) for E. longifolia, L. pumila, and O. stamineus, used to validate the species identification for herbal products. PCR–RFLP was developed for rapid identification using restriction enzyme.....

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Horseshoe crab populations are said to be declining worldwide. However, there is still no published  report on the status of horseshoe crabs in Malaysia. In this study, microsatellite markers were  developed using 5'anchored ISSR-PCR enrichment procedure to diagnose population genetic structure  of the mangrove horseshoe crab, Carcinoscorpius rotundicauda. A total of 134 microsatellite regions  have been succesfully isolated using this technique and 36 novel microsatellite markers were  developed. Out of these, eleven informative microsatellite loci were identified in an analysis of  127 samples representing five C. rolundicauda populations namely Kuala Juru, Kg. Pennatang, Kg.  Chuah, Kg. Sekokoh and Kg. Sg. Pulai  from Peninsular Malaysia. The mean expected (He) and observed  heterozygosity (Ho) values obtained ranged between 0.299-0.42 l and 0.148-0.197 respectively. Significant departures from Hardy-Weinberg equilibrium were detected m C. rotundicauda populationsÂ.....

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