The objectives of this study are to extract and isolate the alkaloids from two species of Alseodaphne; Alseodaphne peduncularis (Wall. ex Nees) Meisn from Kluang- Mersing, Johor and Alseodaphne corneri Kosterm from University Malaya, Kuala Lumpur. The extraction process of the plant material started by cold percolation process using hexane to remove non-polar organic compounds, waxes and fats. The plant material then re-extracted by dichloromethane using soxhlet extractor followed by acid-base extraction to get the alkaloid crude extract. The isolation and purification of alkaloids from the crude extract were done by using various chromatographic techniques including column chromatography and preparative thin layer chromatography. The elucidation of the isolated alkaloids were determine by using various spectroscopic methods such as 1D NMR (1H, 13C and DEPT) and 2D NMR (COSY, HMQC and HMBC), ultraviolet (UV), infrared (IR) and mass spectrometry (MS). The structures were further confirm.....

851 hits