In Malaysia, Alstonia spathulataBl. is locally known as pulai basong which distribute in peat forest. The separations of the chemical components from hexane crude extract (bark) of Alstonia spathulata were carried out using different chromatographic techniques (column chromatography and thin layer chromatography). One compound were isolated and identified as lupeol acetate (1). This compound have never been reported and isolated from this species. The structures of this compound were elucidated based on the basis of detailed 1D-NMR (1H, 13C and DEPT) and 2DNMR (COSY, HSQC, HMBC) spectroscopic analysis. The data analysis was also compared with the literature...

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The aim of this study was to extract and identify the chemical compounds isolated from Alstonia spathulata and Kopsia singapurensis. The isolated compounds were tested for their biological activities include cytotoxicity (MCF-7 cell line), antibacterial (Bacillus cereus) and antioxidant (DPPH). The separation of the chemical components from both species was carried out using different chromatographic techniques (column chromatography and thin layer chromatography). The structures of isolated compounds have been elucidated through spectral analysis including 1D-NMR (1H, 13C and DEPT), 2D-NMR (1H-1H COSY, HSQC/HMQC and HMBC), IR, UV, MS (GCMS) and also by comparison with previous literature data. Studies on the chemical constituents of the bark of Alstonia spathulata has led to the isolation of five triterpenes; β-amyrin 113, β-amyrin acetate 114, β-sitosterol 115, lupeol acetate 116, stigmasterol 117 and a mixture of three triterpenes (lupeol 118, α-amyrin 119 and β-amyrin 113). Fo.....

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In Malaysia, Alstonia spathulata Bl. is locally known as ‘Pulai Basong’ which distribute in peat forest. The separation of the chemical components from hexane and DCM crude extract of Alstonia spathulata was carried out using column chromatography (CC) and thin layer chromatography (TCL) techniques. A total of five known compound were isolated from the stem bark extracts and identified as: β-amyrin, β-amyrin acetate, lupeol acetate, stigmasterol and β-sitosterol. The elucidation structures of these compounds were established using 1D-NMR (1H, 13C and DEPT) and 2D-NMR (COSY, HSQC, HMBC) spectroscopic analysis, involving also comparison with data from the literature...

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