UPSI Digital Repository (UDRep)
|
UPSI Digital Repository (UDRep)
|
|
|
| Total records found : 8 |
| Simplified search suggestions : Wong Chee Fah |
| 1 | 2017 article | Effect of CEO power on Malaysian firm performance Wong Yin Theng, Hooy Chee-Wooi, 762 hits |
| 2 | 2017 article | C-Terminal splice variants Of P/Q-type Ca2+ channel Cav2.1 subunits are differentially regulated by Rab3-interacting molecule proteins Wong Chee Fah, Hirano Mitsuru, Takada Yoshinori, Yamaguchi Kazuma, Kotani Hiroshi, Kurokawa Tatsuki, Mori Masayuki X., Snutch Terrance P., Ronjat Mich 585 hits |
| 3 | 2017 article | Kodalys teaching method increasing preschool childrens solfege singing skills Luen Loy Chee, Ayob Aminah, Wong Colleen, Augustine Christine, 2155 hits |
| 4 | 2017 article | Construction of new genetic tools as alternatives for protein overexpression in escherichia coli and pseudomonas aeruginosa Wong Chee Fah, Raja Abd. Rahman Raja Noor Zaliha, Basri Mahiran, Salleh Abu Bakar, 1081 hits |
| 5 | 2017 article | New azafluorenone derivative and antibacterial activities of alphonsea cylindrica barks Abdul Talip Munirah, Syed Abdul Azziz Saripah Salbiah, Wong Chee Fah, Awang Khalijah, Naz Humera, Mhd Bakri1 Yuhanis, Ahmad Mohamad Syahrizal, Litaudo 1933 hits |
| 6 | 2019 article | Characterization of thermostable aminoacylase from Geobacillus sp. strain SZN Wong, Chee Fah Aminoacylase (EC 3.5.1.14) hydrolyzes N–acetylated amino acids to produce amino acids. Although thermostable aminoacylase has been commercially produced since 2004, there was a knowledge gap in the field of understanding aminoacylase thermostability from a structural point of view. This study investigated the physical and structural properties of the purified thermostable aminoacylase SZN. The spectropolarimetry data for structural determination has indicated a gradual decrease of α-helix from 36 to 27.6%, followed by tremendous disorientation of the structure at the transition of temperatures from 60 to 70°C (27.6 to 19.5%). In contrast, the percentage of β-sheet has increased steadily over the tested temperatures. The α-helix, where notable metal binding and catalytic residues are located, was totally weakened at temperatures above 70?C, thus resulted in loss of activity. The loss of the α-helical structure could further explain drastic deterioration of activity at temperature..... 898 hits |
| 7 | 2019 article | Contribution of coiled-coil assembly to Ca2+/ calmodulin-dependent inactivation of TRPC6 channel and its impacts on FSGS-associated phenotypes Wong, Chee Fah Background TRPC6 is a nonselective cation channel, and mutations of this gene are associated with FSGS. These mutations are associated with TRPC6 current amplitude amplification and/or delay of the channel inactivation (gain-of-function phenotype). However, the mechanism of the gain-of-function in TRPC6 activity has not yet been clearly solved.
Methods We performed electrophysiologic, biochemical, and biophysical experiments to elucidate the molecular mechanism underlying calmodulin (CaM)-mediated Ca2+-dependent inactivation (CDI) of TRPC6. To address the pathophysiologic contribution of CDI, we assessed the actin filament organization in cultured mouse podocytes.
Results Both lobes of CaMhelped induce CDI.Moreover, CaM binding to the TRPC6 CaM-binding domain (CBD) was Ca2+-dependent and exhibited a 1:2 (CaM/CBD) stoichiometry. The TRPC6 coiled-coil assembly, which brought two CBDs into adequate proximity, was essential for CDI. Deletion of the coiled-coil slowed CDI of TRPC6, indica..... 540 hits |
| 8 | 2021 article | Development of genetic tools for protein overexpression from pseudomonas Wong, Chee Fah Overexpression of a number of industrially- and medically-important proteins is always a challenge for most researchers as genes from bacterial hosts especially from the opportunistic pathogen, Pseudomonas aeruginosa, as well as from other Pseudomonads were reported to be poorly expressed in E. coli. Examples continue to be published demonstrating that certain Pseudomonas proteins cannot be expressed at satisfactory levels in E. coli owing to their promoter variations, absence of specific molecular activators and poor protein translation. Nevertheless, the extension of E. coli-based technology to Pseudomonas can be used to overcome such barriers. In this chapter, the evolution and modification of genetic tools ranging from types of promoters, elements for interspecies transfer, origins of replications, regulatory genes, genes facilitating in genome integration and host strains, used in the construction of Escherichia-Pseudomonas shuttle expression vectors will be explored and discussed..... 614 hits |
