UPSI Digital Repository (UDRep)
Start | FAQ | About


Browse by: Year_icon Subject Year_icon Publisher Year_icon Year
Total records found : 1
Simplified search suggestions : Nurul Fizatul Nabilah Osman
12023
thesis
Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia
Nurul Fizatul Nabilah Osman
The objective of this study is to develop species-specific environmental DNA (eDNA) markers for Cichla spp. based on mitochondrial cytochrome oxidase I (COI) gene sequences. Validation of the developed species-specific eDNA markers were done exsitu for environmental sample from Tasik Raban. New digital PCR (dPCR) method was also employed to detect the presence of Cichla ocellaris, and Cichla kelberi in environmental water samples. Result from this study showed that COI gene can be used to develop species-specific markers for species Cichla. Ex-situ validation results also showed that the developed markers CO1 amplified specifically to specimen C. ocellaris while CK1 amplified directly to specimen of C. kelberi. In addition, dPCR supported the specificity analysis as absolute fluorescent quantification detected the presence of C. ocellaris and C. kelberi eDNA in water sample at 90% detection confidence. In conclusion, CO1 and CK1 markers were successfully developed and specif.....

75 hits

Filter
thesis ... (1)



Specific Period







Top 5 related keywords

+Nurul +Fizatul +Nabilah +Osman




Recently Access Item

No recently access item.


Installed and configured by Bahagian Automasi, Perpustakaan Tuanku Bainun, Universiti Pendidikan Sultan Idris
If you have enquiries, kindly contact us at pustakasys@upsi.edu.my or 016-3630263. Office hours only.