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Type :thesis
Subject :SD Forestry
Main Author :Siti Zubaidah Lood
Title :In vitro morphogenic response from different explants of sweet basils, Ocimum basillicum L.
Place of Production :Tanjong Malim
Publisher :Fakulti Teknikal dan Vokasional
Year of Publication :2018
Corporate Name :Universiti Pendidikan Sultan Idris
PDF Guest :Click to view PDF file

Abstract : Universiti Pendidikan Sultan Idris
The purpose of this study was to examine the ability of seed germination Ocimum basilicum L. to germinate through tissue culture, the rate of time difference in seed germination in traditional or modern technology. In addition, callus formation was also carried out in the study to see the rate of cell differentiation at the exploratory part. At the end of the study, it was also tested by acclimatization of the explant to green house. The complete regeneration of the Ocimum basilicum L. plant and callus production was successfully produced through the tissue culture technique. The part of leaves, stems and roots of 8-week aseptic seedlings were used in this experiment. All cultures are stored at a temperature of 25 ± 1ºC and illuminated with fluorescent light for 16 hours of light, 8 hours dark. Plant acclimatization is done on three different soil types, namely garden soil, coconut powder and vermiculation. After completion, all data was recorded and analyzed using ANNOVA. Using MS medium (Murashige and Skoog, 1962) root explants were found to be very responsive for 0.5 mg / l BAP and 1.0 mg / l NAA concentration to produce maximum callus weight, and The stem explant for a concentration of 0.5 mg / l BAP with 1.5 mg / l of NAA will produce double shoots. Growth of seedlings are most optimum when regenerated from stem explants are acclimated in a garden with the survival rate of 88 ± 0.8%. As the conclusion, this method of tissue culture is the best method in producing new generation of Ocimum basilicum L. and its features are preserved. The implications of this study suggest that this tissue culture method can help to regenerate this species from extinction and the benefits of its use can continue to apply.

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