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Abstract : Universiti Pendidikan Sultan Idris |
The purpose of this study was to examine the ability of seed germination Ocimum
basilicum L. to germinate through tissue culture, the rate of time difference in seed
germination in traditional or modern technology. In addition, callus formation was also
carried out in the study to see the rate of cell differentiation at the exploratory part. At
the end of the study, it was also tested by acclimatization of the explant to green house.
The complete regeneration of the Ocimum basilicum L. plant and callus production was
successfully produced through the tissue culture technique. The part of leaves, stems
and roots of 8-week aseptic seedlings were used in this experiment. All cultures are
stored at a temperature of 25 ± 1ºC and illuminated with fluorescent light for 16 hours
of light, 8 hours dark. Plant acclimatization is done on three different soil types, namely
garden soil, coconut powder and vermiculation. After completion, all data was recorded
and analyzed using ANNOVA. Using MS medium (Murashige and Skoog, 1962) root
explants were found to be very responsive for 0.5 mg / l BAP and 1.0 mg / l NAA
concentration to produce maximum callus weight, and The stem explant for a
concentration of 0.5 mg / l BAP with 1.5 mg / l of NAA will produce double shoots.
Growth of seedlings are most optimum when regenerated from stem explants are
acclimated in a garden with the survival rate of 88 ± 0.8%. As the conclusion, this
method of tissue culture is the best method in producing new generation of Ocimum
basilicum L. and its features are preserved. The implications of this study suggest that
this tissue culture method can help to regenerate this species from extinction and the
benefits of its use can continue to apply. |
References |
Abobkar I. M. Saad & Ahmad M. Elshahed (2012), Recent Advences in Plant in vitro Culture, Chapter 2 : Plant Tissue Culture Media (pp. 22-40), INTECH
Acheapong E. (1982). Multiplication and Conservation of Cocoyam (Xarthosoma sajiltifoljum) germplasm by application of tissue culture methods Ph.D Thesis. University of Burmingham, UK
Aladele S. E. & Kuta D. (2008). Environmental and genotypic effects on the rowth rate of in vitro cassava plantlet (Manihot esceulenta) African Journal of Biotechnology Vol. 7 (4), pp. 381-385.
Aloni, R. (1980). Role of auxin and sucrose in the differentiation of sieve and tracheary elements in plant tissue cultures. Planta. 150: 255 263.
Arditti, J., & Ernst, R. (1993). Micropropagation of Orchids. John Wiley & Sons, Inc, Canada, pp:1-665
Bapat, V.A., Mhatre, M. and Rao, P.S. (1987). Propagation of Morus indica (mulberry) by encapsulated shoot buds. Plant Cell Rep. 6: 393-395.
Bhojawi, S.S., & Razdan, M.K. (1996). Plant Tissue Culture: Theory and Practise, a Revised Edition, Elsever, Amsterdam, pp:39-62
Bhojwani, S.S. & Razdan, M.K. (1992). Plant tissue culture: Theory and practice. Elsivier, Amsterdam, London, New York, Tokyo.
Biren N. S., Avinash K. S. (2010), Pharmacognostic Studies of The Lagenaria siceraria (Molina) Standley, Gujerat, India
Butenko, R.G. (1968). Plant tissue culture and plant morphogenesis. Translated from Russian.Israel program for Scientific Translation, Jerusalem.
Capuano C, Piccioni E, Standardi A (1998). Effect of different treatments on the conversion of M.26 apple rootstock synthetic seeds obtained from encapsulated apical and axillary micropropagated buds. J. Hort. Sci. 73:299-305.
Carew,D.P., & Staba, E.J. (1965). Plant tissue culture; its fundamentals, application and relationship to medicinal plant studies. Lloydia. 28: 1-26.
Cassells, A.C.(1991). Problems in tissue culture: Culture contamination. In: Micropropagation Technology tind Application. P.C. Debergh and R.H. Zimmerm, cds. Kluwer Academic Publishers. Dordrecht, Netherlands. pp. 31-44.
Chaleff, R. S., and P. S. Carlson. (1974). Somatic cell genetics of higher plants. Ann. Rev. Genet. 8: 267-278.
Chan , P.Y., Fujita, S., Kohno, K., Ashrafuzzaman, M.D., Nakamura, N. and Hayashi, N. (2000). Purification and characterization of polyphenol oxidase from banana (Musa sapietum L.) pulp. Journal of Agricultural and Food Chemistry 48: 2732- 2735.
Cocking, E. C. 1972. Plant cell protoplasts-isolation and development. Ann. Rev. Plant Physiol. 23: 29-50.
Conger, B. V. (1980). Cloning Agricultural Plants via In-vitro Techniques, CRC Press Inc, Boca Raton, Florida, pp; 1-50
Dalmacio I. F. (1992). Biotechnology for sustainable Agriculture National Institute of Biotechnology and Applied Microbiology, University of the Philippines.Dalziel, J. M. 1937. The useful plants of West Tropical Africa. Whitefriars Press, London, 612 pp.
Daniel, R.L. (1998). The many dimension of plant tissue culture research. Webmaster of Aggie Horticulture Publications, pp.201-210.
de Fossard R.(1976) Tissue culture for plant propagation. Armidale: University of New England.
Driver, J.A., & Kuniyuki, A.H. (1984) In vitro propagation of paradox walnut rootstock. Hotr. Sci., 19: 507-709.
Encyclopedia of Life (2009), Introduction. http://eol.org/pages/281/overview
Enjalric, F., Carron, M.P., Lardet, L.(1988). Contamination of Primary Cultures in Tropical Areas: The Case of Hevea Brasiliensis. In:Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures. ISHS Acta Horticulturae. 225:
Fabijan D, Taylor JS & Reid DM (1981) Adventitious rooting in hypocotyls of sunflower (Helianthus annuus) seedlings II. Action of gibberellins, cytokinins, auxins and ethylene. Physiologia Plantarum 53: 589?597.
Food and Agriculture Organization (FAO). (2005). Statistics Database (Agriculture Data) 2001. On internet http:/apps.fao.org.
Frusciante, L., Barone, A.,Carputo,D. Ercolano, M.R., Della Rocca, F., & Esposito, S. (2000). Evaluation and use of Plant Biodiversity for Food and Pharmaceutical. Fitoterpia. 71 : 66 – S72.
Gamborg, O., Miller, R. & Ojima, K. 1968). Nutrient requirement suspensions cultures of soybean root cells. Experimental Cell Research. 50(1):151-158.
Gautheret, R. J. (1934). Culture du tissus cambial. Comptes Rendus Hebdomadaires des Séances de l'Académie des Sciences, 198, 2195–2196.
Gautheret, R. J. (1935). Recherches sur la culture des tissus végétaux. Ph.D. Thesis, Paris
Gautheret, R. J. (1939). Sur la possibilité de réaliser la culture indéfinie des tissus de tubercules de carotte. Comptes Rendus Hebdomadaires des Séances de l'Académie des Sciences, 208, 118–120.
Gbile Z.O.(1998). Collection, Conservation and Utilization of medicinal plants. In Robert PA, & Janice EA (eds.). Conservation of Plant Genes III: Conservation and Utilization of African Plants. Missouri Botanical Gardens Press. pp. 163-17
Georges, D., Chenieux, J.C. & Ochatt. S.J. (1993). Plant regeneration from aged-callus of the woody ornamental species Lonicera japonica cv. "Hall's prolific". Plant Cell Reports. 13:91-94. Growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199-204.
Han, J-S., Oh, D-G., Mok, I-G., Park, H-G., Kim, C. K., (2004), Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.). Plant Cell Rep., 23:291-296 Improvement of cucurbits; In: Biology and utilization of the cucurbitaceae; pp. 367?381 eds. D M Bates, R W Robinson & C Jeffrey(Ithaca, London: Cornell Univ. Press).
Hartmann, H.T., Kester, D.E. & Davies, F.T. (1990). Plant propagation: principles and practices. Englewood Cliffs, NJ: Prentice Hall.
IUCN (2006). IUCN Red List of Threatened Species. IUCN, Gland, Switzerland
Kanchanapoom, K., Jantaro, S. & Rakchad, D. (2001). Isolation and fusion of protoplasts from mesophyll cells of Dendrobium pompadour. Science Asia. 27: 29-34.
Kane, M. (2003). Bacterial and fungal indexing of tissue cultures. http://www.hos.ufl.edu/
Nwe, N., T., & Tamur, H. (2010). Production of Fungal Chitosan by Enzymatic Method and Applications in Plant Tissue Culture and Tissue Engineering: 11 Years of Our Progress, Present Situation and Future Prospects. Biopolymers. doi:10.5772/10261
Kasha, K. J. (1974). Haploids in Higher Plants. University of Guelph, Guelph.
Katare, C., Agrawal, S., Jain, M., Rani, S., Saxena, S., Bisen, P.S., Prasad, G.B.K.S. (2011). Lagenaria siceraria : A Potential Source of Anti-Hyperlipidermic and Other Pharmacological Agents. Current Nutrition & Food Science Vol. 7, No 3, pp 1- 9
Krikorian AD (1995) Hormones in tissue culture and micropropagation. In: Plant hormones: physiology, biochemistry and molecular biology (P.J. Davies, ed.). Kluwer Academic Publishers, Dordrecht, p. 774?796.
Lee, J.M. (1994). Cultivation of grafted vegetables I. Current status, grafting methods, and benefits. HortSci. 29: 235-239.
Lee, J.M. (2003). Advances in vegetable grafting. Chronica Horticulturae. 43:13-19.
Lloyd, G. & McCown, B. (1980) Commercially-feasible micropropagation of mountainlaurel, Kalmia latifolia, by use of shoot-tip culture. Intern. Plant Prop. Soc. Proc. 30: 421–427.
Mendi, Y.Y., Ipek, M., Buzkan, N., Kacar, Y. A., Curuk, S., (2009), Regeneration and Histological Analysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.). Turk J Agric For 33(2009) pp. 165-172
Moreno V & Roig LA (1990) Somaclonal variation in cucurbits; In: Biotechnology in agriculture and forestry Vol 11: Somaclonal variation in crop improvement I; pp 435?464 ed. Y P S Bajaj (Berlin, Heidelberg:Springer? Verlag).
Murashige T, Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant.; 15: 473-479.
Murashige, T. (1974). Plant propagation through tissue cultures. Ann. Rev. Plant Physiol. 25: 135-166.
Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco cultures, Physiol. Plant. 15:473-497
Murashige, T.; Skoog, F. (1962), A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15, 473-497.
Nakamura, K., Wakita, Y. & Yokota, S. (1995). Induction of multiple shoots by shoot apex culture in Magnolia obovata Thunb. Plant Tissue Culture Letters. 12: 34- 40.
Nishibayashi, S., H. Kaneko & T. Hayakawa. (1996). Transformation of cucumber (Cucumis sativus L.) plants using Agrobacterium tumefaciens and regeneration from hypocotyl explants. Plant Cell Rep. 15: 809-814.
Nitsch JP 1951. Growth and development in vitro of excised ovaries. Am. J. Bot. 38: 566?577.
Nitsch, J.P. & Nitsch, C (1969) Haploid plants from pollen grains. Science. 163: 85–87
Nobécourt, P. (1939). Sur la pérennité et l’augmentation de volume des cultures de tissues végétaux. Comptes Rendus des Séances de la Société de Biologie et de ses Filiales, 130, 1270–1271.
Okere, A. U. & Adegeye, A. (2011). In vitro propagation of an endangered medicinal timber species Khaya grandifoliola C. Dc. African Journal of Biotechnology Vol. 10(17), pp. 3335-3339
Philips, Greogory, C. (2004). In vitro morphogenesis in plants – recent advances. In Vitro Cellular and Development Biology – Plant. 40: 342-345
Pierik, R. I. M. (1987). In vitro Culture of Higher Plants. Martinus Nijhoff Publishers, Dordrecht, ppt:1-344
Puhan, Z., & Martin. S. M. (1971). The industrial potential of plant cell culture. Prog. Ind. Microbiol. 9: 13-39.
Reddy M. C., Murthy K. S. R & Pullalah T (2012), Synthetic seeds : A review in agriculture and forestry, African Journal of Biotechnology Vol. 11(78), pp. 14254-14275
S. Habibur Rahman (2003), Bottle Gourd (Lagenaria siceraria) A vegetable good for health, Research and Development, Amal Herbal Formulation Pvt. Ltd., M. G. Road, Ghatkopar, Mumbai
Saidin, I. Sayuran tradisional, ulam dan penyedap rasa (2000). Penerbit Universiti kebangsaan Malaya. pp 1-33
Sarowar S, Oh HY, Hyung NI, Min BW, Harn CH,Yang SK, Ok SH & Shin JS (2003) In vitro micropropagation of a Cucurbita interspecific hybrid cultivar ? a root stock plant. Plant Cell Tiss. Organ Cult. 75: 179?182.
Sarowar, S., H.Y. Oh, N.I. Hyung, B.W. Min, C.H. Harn, S.K. Yang, S.H.Ok & J.S. Shin. (2003). In vitro propagation of a Cucurbita interspecific hybrid cultivar-a root stock plant. Plant Cell, Tissue Org Cult. 75: 179-182.
Schenk, R. V., & A. C. Hildebraudt. 1972. Medium and techniques for induction and Skoog, F., & Miller, C.O.(1957). Chemical regulation of growth and organ formation in plant tissue cultured in vitro . Symp. Exp Biol. 11:118-131.
Skoog, F., Miller, C.O (1957). Chemical regulation of growth and organ formation in plant tissue cultured in-vitro. Symp. Exp Biol. 11:118-131
Steiner AA, Winden H. (1970) Recipe for ferric salts of ethelenediaminetetracetic acid. Plant Physiol.; 46: 862- 863.
Steward, F.C., Mapes, M.O., & Mears, K. (1958). Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells, American Journal of Botany. 45:705-708
Street, H.E. (1973). Plant tissue and cell culture. Botanical Monogr. Blackwell Scientific Publications, London. 2.
Taji, A., Prakash, P.K. & Prakash, L. (2002). In Vitro Plant Breeding. Howarth Press Inc. New York
Tempe, J., Ed. (1972). Protoplastes et Fusion de Cellules Somatiques Vegetales. Colloq. Int. Cent. Natl. Rech. Sci., Paris. 222.
Thomas, D.T & Philip B. (2005). Thidiazuron-induced high frequency shoot organogenesis from leaf-derived callus of a medicinal climber,Tylophora indica (Burm.F) Merrill.In Vitro Cell .Dev.Biol- Plant. 41:124-128.
Thomas, T.D. & Jacob, A., 2004. Direct somatic embryogenesis of Curculigo orchioides Gaertn. an Endangered medicinal herb. J. Plant Biotech., 6 :193-197.
Thorpe, T. A. (1990). The current status of plant tissue culture. In S. S. Bhojwani (Ed.), Plant tissue culture: Applications and limitations (pp. 1–33). Amsterdam: Elsevier.
Torres KC. (1989), editor. Tissue culture techniques for horticultural crops. New York, London: Chapman and Hall.
Torres, K.C. (1989). Tissue culture techniques for horticultural crops. AVI Books, New. York.
Vasil, I. K., & V. Vasil. (1972) Totipotency and embryogenesis in plant cell and tissue cultures. In Vitro. 8: 117-125.
Vinterhalter D, Vinterhalter BS. Micropropagation of Dracaena sp. (1997)In: Bajaj YPS (ed.) Biotechnology in agriculture and forestry 40, High-tech. and Micropropagation VI. Berlin, Heidelberg: Springer; . p131- 146.
Wehner TC, Cade RM & Locy RD (1990) Cell, tissue and organ culture techniques for genetic
White, P. R. 1963. The Cultivation of Animal and Plant Cells. 2nd ed. Ronald Press, New York.
White, P.R. (1943). A Handbook of Plant Tissue Culture. Jacques Cattell Press, Lancaster, PA. pp:227
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