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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| Chromolaena odorata has reported to have medicinal value due to the presence of
active compounds which have antimicrobial and wound healing properties. Therefore,
this study was carried out to formulate the antimicrobial cream from C. odorata
extract used for wound healing treatment. The study aims to screen the secondary
metabolites and determine C. odorata extract concentration for antimicrobial activity
to be used in cream formulation. The leaves were extracted in 95% methanol and 95%
ethanol for secondary metabolite screening and Gas Chromatography-Mass
Spectrometry. Chromolaena odorata extract with a concentration of 5%, 10%, 15%
and 20% was performed for antimicrobial activity tested on Staphylococcus aureus,
Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Candida albicans.
The best of C. odorata extract concentration was used in six formulations of
antimicrobial cream. The stability test was performed on all cream formulations. The
methanolic extract was present with tannin, terpenoid, phenolic acids, alkaloid, and
saponin while ethanolic extract was present with tannin and alkaloid. Both extracts
are absent with flavonoid compounds. The GC-MS chromatogram identified as many
as 26 and 15 compounds for ethanolic and methanolic extract, respectively. The C.
odorata ethanolic extract consists mainly of Fatty acid (39.2%), Sesquiterpenoid
(20.69%) and Phenol (0.51%). Meanwhile, C. odorata methanolic extract consists of
Sesquiterpenoid (62.26%) and Fatty acid (17.11%). The best concentration was 20%
C. odorata methanolic extract had the highest diameter of inhibition zone against E.
coli and C. albicans at 20.67 mm and 13.3 mm, respectively. Formulation of F4, F5,
and F6 was stable in physicochemical and organoleptic studies. No significant
difference for F1 and F3 in pH measurement; F2, F3, F5 and F6 in antibacterial
activity and F1, F3, and F5 in antifungal activity. In conclusion, out of the six
formulations, the F3 formulation has optimal stability and is suitable as an
antimicrobial cream for wound healing treatment. |
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