Bioactive chemical constituents of three crude extracts of Polyalthia Sclerophylla using GC-mass and phytochemical screening and their antibacterial and cytotoxicity activities

Ismail Zainol

Abstract

The aim of the present study was to extract of Polyalthia sclerophylla leave (LPS) and identify its chemical constituents using Gas chromatography-Mass (GS-Mass) spectrometry and classical phytochemicals methods. This study included as well the evaluated of the bio-medical properties of LPS using antibacterial (against six bacteria species) and cytotoxicity (M-63 human cell line) activities. Three different solvent system i.e. methanol (MeOH), dichloromethane (DCM) and hexane were used to extract the LPS, three samples were obtained and labelled as MLPS, DLPS, and HLPS, respectively, and evaluated their biomedical activities. Phytochemical screening results showed to present glycosides and terpenoids in MLPS, DLPS, and HLPS, while alkaloids did not detect the presence in all extracts. GC-Mass results were detected to present 21 chemical compounds, the higher percentages were cyclobutanone, 2-methyl-2-oxiranyl-, 2-Undecanol and Pyridine, 2,3,4,5-tetrahydro-3-methyl-, while 1,6-Heptadiene, 1,1,3-Trimethylcyclopentane, and 2(5H)-Furanone, 5-methyl were detected to be presented with low percentages. Alamar blue assay was used to evaluate the LPS cytotoxicity and there was no-toxic effect for all concentrations with higher cell availability average 99.5 %. MLPS, DLPS, and HLPS were showed significant effect to inhibition the bacteria growth, MLPS showed more effect than DLPS and HLPS to be used as a bacterial agent. Current study was establishment for chemical and biomedical properties of LPS and showed good biomedical properties with non-toxic effect. Copyright 2023 by SPC (Sami Publishing Company)

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