Bioactive chemical constituents of three crude extracts of Polyalthia Sclerophylla using GC-mass and phytochemical screening and their antibacterial and cytotoxicity activities

Ismail Zainol

QR Code Link :
Type :	article
Subject :	Q Science (General)
ISSN :	2717-0535
Main Author :	Ismail Zainol
Title :	Bioactive chemical constituents of three crude extracts of Polyalthia Sclerophylla using GC-mass and phytochemical screening and their antibacterial and cytotoxicity activities

Place of Production :	Tanjung Malim
Publisher :	Fakulti Sains dan Matematik
Year of Publication :	2023
Notes :	Eurasian Chemical Communications
Corporate Name :	Universiti Pendidikan Sultan Idris
HTTP Link :	Click to view web link

Abstract : Universiti Pendidikan Sultan Idris

The aim of the present study was to extract of Polyalthia sclerophylla leave (LPS) and identify its chemical constituents using Gas chromatography-Mass (GS-Mass) spectrometry and classical phytochemicals methods. This study included as well the evaluated of the bio-medical properties of LPS using antibacterial (against six bacteria species) and cytotoxicity (M-63 human cell line) activities. Three different solvent system i.e. methanol (MeOH), dichloromethane (DCM) and hexane were used to extract the LPS, three samples were obtained and labelled as MLPS, DLPS, and HLPS, respectively, and evaluated their biomedical activities. Phytochemical screening results showed to present glycosides and terpenoids in MLPS, DLPS, and HLPS, while alkaloids did not detect the presence in all extracts. GC-Mass results were detected to present 21 chemical compounds, the higher percentages were cyclobutanone, 2-methyl-2-oxiranyl-, 2-Undecanol and Pyridine, 2,3,4,5-tetrahydro-3-methyl-, while 1,6-Heptadiene, 1,1,3-Trimethylcyclopentane, and 2(5H)-Furanone, 5-methyl were detected to be presented with low percentages. Alamar blue assay was used to evaluate the LPS cytotoxicity and there was no-toxic effect for all concentrations with higher cell availability average 99.5 %. MLPS, DLPS, and HLPS were showed significant effect to inhibition the bacteria growth, MLPS showed more effect than DLPS and HLPS to be used as a bacterial agent. Current study was establishment for chemical and biomedical properties of LPS and showed good biomedical properties with non-toxic effect. Copyright 2023 by SPC (Sami Publishing Company)

References

S. Saepou, M. Pohmakotr, V. Reutrakul, C. Yoosook, J. Kasisit, C. Napaswad, P. Tuchinda, Anti-HIV‐1 diterpenoids from leaves and twigs of Polyalthia sclerophylla, Planta Medica, 2010, 76, 721-725. [crossref], [Google Scholar], [Publisher].

P.T. Sheba, J.V. Devasia, E. Joseph, Phytochemical screening and chromatographic identification of acetogenin in Annona glabra L. leaves, International Journal of Crrent Research in Chemistry and Pharmaceutical Sciences, 2022, 9, 1-7. [crossref], [Google Scholar], [Publisher].

M.A.K. Zaman, A.M. Azzeme, S.N. Ramli, N.A. Shaharuddin, S. Ahmad, S.N. Abdullah, Solvent extraction and its effect on phytochemical yield and antioxidant capacity of woody medicinal plant, Polyalthia Bullata. BioResources, 2020, 15, 9555. [crossref], [Google Scholar], [Publisher].

Y.C. Chen, Y.C. Chia, B.M. Huang, Phytochemicals from Polyalthia species: Potential and implication on anti-oxidant, anti-inflammatory, anti-cancer, and chemoprevention activities, Molecules, 2021, 26, 5369. [crossref], [Google Scholar],[Publisher].

R. Appiah-Opong, K. Agyemang, E. Dotse, P. Atchoglo, K.B. Owusu, A. Aning, M. Sakyiamah, R. Adegle, F. Ayertey, A.A. ppiah, A.K. Nyarko, Anti-plasmodial, Cytotoxic and antioxidant activities of selected Ghanaian medicinal plants, J. Evid.-Based Integr. Med., 2022, 27, 2515690X211073709. [crossref], [Google Scholar], [Publisher].

M.V. Nguyen, J.W. Han, Q. Le Dang, S.M. Ryu, D. Lee, H. Kim, G.J. Choi, Clerodane diterpenoids identified from Polyalthia longifolia showing antifungal activity against plant Pathogens, J. Agric. Food Chem., 2021, 69, 10527-10535. [crossref], [Google Scholar], [Publisher].

M.U. Mudhafar, I. Zainol, Medical values, antimicrobial, and anti-fungal activities of Polyalthia genus, Int. J. Pharm. Res., 2019, 11, 90-96. [Google Scholar], [Publisher].

R.Y. Siddappa, S.J. Aditya Rao, B.M. Usha, B. Verma, P. Mahadevappa, Anti-proliferative activity of Labdane Diterpenes isolated from Polyalthia cerasoides and their molecular interaction studies, Curr. Drug Discov. Technol., 2022, 19, 78-85. [crossref], [Google Scholar], [Publisher].

I.W. Lo, G.Y. Liao, J.C. Lee, C.I. Chang, Y.C. Wu, Y.Y. Chen, S.P. Liu, H.J. Su, C.I. Liu, C.Y. Kuo, Z.Y. Lin, Novel Aporphine-and Proaporphine–Clerodane Hybrids Identified from the Barks of Taiwanese Polyalthia longifolia (Sonn.) Thwaites var. pendula with Strong Anti-DENV2 Activity, Pharmaceuticals, 2022, 15, 1218. [crossref], [Google Scholar], [Publisher].

M. Mudhafar, I. Zainol, S. Desa, C.N. Jaafar, Mini-review of phytochemistry for polyalithia Longiflia, Eurasian J. Anal. Chem. 2019, 14, 119-147. [Google Scholar], [Publisher].

M. Mudhafar, H.A. Alsailawi, C.N. Jaafar, Synthesis, characterisation, cytotoxicity and antibacterial studies of green synthesised silver nanoparticles using leaves of Polyalthia Sclerophylla, Malays. J. Microsc., 2022, 18, 79-91. [Google Scholar], [Publisher].

G. Baba, K.M. Novidzro, A. Kpoezoun, Physicochemical study, Fatty acid profile and antioxidant potential of the seed oil of Polyalthia longifolia angustifolia, Chem. Sci. Int. J., 2022, 38-46. [crossref], [Google Scholar], [Publisher].

F. Adaramola, R. Cooposamy, O.O. Olajuyigbe, Antimicrobial activity, bioactive constituents, and functional groups in aqueous methanol extract of Polyalthia longifolia (Sonn.) thwaites leaves, Pharmacognosy Magazine, 2021, 17, 594-604. [Google Scholar], [Publisher].

I. Zainol, M.A. Zainurin, N.H. Bakar, C.N. Jaafar, M. Mudhafar, Characterisation of porous Hhydroxyapatite beads prepared from fish scale for potential bone filler applications, Malays. J. Microsc., 2022, 18, 48-57. [Google Scholar], [Publisher].

C.A. Nuraskin, R. Idroes, C. Soraya, Identification of secondary metabolite using phytochemical and infra-radiation test on the leaves of vitex pinnata found in the Seulawah Agam mountain region of Aceh, Res. J. Pharm. Technol., 2019, 12, 5247-5250. [crossref], [Google Scholar], [Publisher].

R.K.M. Jawad, S.S. Ayat, M.M. Karhib, H.M. Almusawi, H.A. Raheem, H.A. Alsailawi, M. Mudhafar, Diagnostic study of the patients with celiac disease via using duodenal biopsy depending on intraepithelial lymphocytes alone, Eur. Chem. Bull., 2022, 11, 10-10 [crossref], [Google Scholar], [Publisher].

M.A.N. Zainurin, I. Zainol, M. Mudhafar, Biogenic synthesis of silver nanoparticles using Neem Leaf extract as reducing agent and hydrolyzed collagen as stabilizing agent, Malays. J. Microsc., 2022, 18. [Google Scholar], [Publisher].

E. Nortjie, M. Basitere, D. Moyo, P. Nyamukamba, Extraction methods, quantitative and qualitative phytochemical screening of medicinal plants for antimicrobial textiles: a review, Plants, 2022, 11, 2011. [crossref], [Google Scholar], [Publisher].

N. Bhalla, N. Ingle, S.V. Patri, D. Haranath, Phytochemical analysis of Moringa oleifera leaves extracts by GC-MS and free radical scavenging potency for industrial applications, Saudi J. Biol. Sci., 2021, 28, 6915-6928. [crossref], [GoogleScholar], [Publisher].

H.T. Qaddoori, A. Hassan, M.S. Al-shoky, Y.K Kalil, M. Mudhafar, Comparative study of sme biochemical and Immunological parameters of patients with COVID-19 disease and non-infected people, Egypt. J. Chem., 2022, 65, 217-223. [crossref], [Google Scholar], [Publisher].

This material may be protected under Copyright Act which governs the making of photocopies or reproductions of copyrighted materials.
You may use the digitized material for private study, scholarship, or research.

Back to previous page