UPSI Digital Repository (UDRep)
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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| Paraphalaenopsis labukensis Shim, A. Lamb & C.L. Chan (P. labukensis) is an endangered monopodial epiphytic orchid threatened due to habitat fragmentation and overharvesting. Consequently, this research aimed to achieve in vitro propagation of P. labukensis through asymbiotic seed germination and seedling development. This study focused on identification and optimizing capsule maturity, basal media and nutrient requirements. In addition, after hand pollinating the plant, their capsules flowers, length, girth, and colour variations were recorded weekly to ascertain morphological maturity characters of this species. Murashige and Skoog (MS), Knudson C (KC), and Vaccin and Went (VW) basal media were used to determine seed vegetation at 60, 90, and 120 days. Subsequently, for the proliferation and development of protocorms, the best basal media were those supplemented with different organic additives (coconut water (CW) and banana homogenate (BH)) and plant growth regulators (PGRs). As a result, 120 days after pollination (DAP) was chosen as the best capsule age for germination since the capsules had reached maturity. The highest germination rate was attained after 90 days of culture in Knudson C (KC) medium, with 98.78 0.89%, followed by Murashige and Skoog (MS) medium, with 92.80 3.26%. The KC supplemented with 0.5 mg/L NAA media documented a maximum percentage of 17.25 0.96%. The 5 g/L (w/v) BH recorded a higher protocorm proliferation percentage than CW. Additionally, after 150 days of culture (DOC), this medium combination produced the most leaves, averaging four or five. The result of this present study has successfully established an effective in vitro propagation protocol for this species. 2023 by the authors. |
| References |
Utami, E.S.W.; Hariyanto, S. In vitro seed germination and seedling development of a rare Indonesian native orchid Phalaenopsis amboinensis J.J. Sm. Scietifica 2019, 2019, 8105138. Juiling, S.; Leon, S.K.; Jumian, J.; Tsen, S.; Lee, Y.L.; Khoo, E.; Sugau, J.B.; Nilus, R.; Pereira, J.T.; Damit, A. Conservation assessment and spatial distribution of endemic orchids in Sabah. Borneo. Nat. Conserv. Res. 2020, 5, 136–144. [CrossRef] Wood, J.; Beaman, J.; Beaman, R. The Plants of Mount Kinabalu 2. Orchids; Royal Botanic Garden: Richmond, UK, 1993. Chan, C.L.; Lamb, A.; Shim, P.S.;Wood, J.J. Orchids of Borneo: Introduction and Selection of Species; Print and Co. Sdn Bhd: Kuala Lumpur, Malaysia, 1994; p. 402. Swarts, N.D.; Dixon, K.W. Perspectives on orchid conservation in botanic gardens. Trends Plant Sci. 2009, 14, 590–598. [CrossRef] [PubMed] Lamb, A. The conservation of orchids in Sabah (Malaysian Borneo). In Tropical Botanic Gardens: Their Roles in Conservation and Development; Heywood, V.H.,Wyse Jackson, P.S., Eds.; Academic Press: London, UK, 1991; pp. 308–327. IUCN. The IUCN Red List of Threatened Species. Version 2020-I. 2020. Available online: https://www.iucnredlist.org/ (accessed on 3 March 2023). Basker, S.; Bai, V.N. In vitro propagation of an epiphytic and rare orchid Eria bambusifolia Lindl. Res. Biotechnol. 2010, 1, 15–20. Chen, Y.; Goodale, U.; Fan, X.; Gao, J. Asymbiotic seed germination and in vitro seedling development of Paphiopedilum spicerianum: An orchid with an extremely small population in China. Glob. Ecol. Conserv. 2015, 3, 367–378. [CrossRef] Johnson, T.R.; Kane, M.E. Asymbiotic germination of ornamental Vanda: In vitro germination and development of three hybrids. Plant Cell Tissue Organ Cult. 2007, 91, 251–261. [CrossRef] Kahraman, M.U.; Cullum, F.J. Asymbiotic germination and seedling development of terrestrial orchid Bletilla striata using in vitro and ex vitro Cultures. Hortic. Stud. 2010, 38, 1–14. [CrossRef] Ferreira, W.D.M.; Oliveira, A.M.D.; Viana, J.C.; Suzuki, R.M.; Oliveira, J.R.G.D. Asymbiotic germination, initial development in vitro and acclimatisation of Cyrtopodium paludicolum Hoehne, a Brazilian Savanna orchid species. Rodriguésia 2022, 73, 1–14. [CrossRef] Barrientos, B.A.B.; Fang, J.Y. Influence of photoperiod and culture medium on the speed of asymbiotic seed germination and seedling development in Spathoglottis plicata. HortScience 2019, 54, 1570–1575. [CrossRef] Yang, N.; Wang, D.; Gao, Y.; Hu, E.; Yu, X.; Peng, S.; Ji, J.; Zhang, M.S. An efficient micropropagation protocol, chemical components, and hypoglycemic activity for Cremastra appendiculata (D. Don) Makino pseudobulbs. In Vitro Cell. Dev. Biol.-Plant 2022, 58, 213–224. [CrossRef] Yao, L.; Huang, J.; Zhang, S. An Improved Protocol for Asymbiotic seed germination and seedling development of Paphiopedilum tigrinum. Horticulturae 2021, 7, 298. [CrossRef] Dutra, D.; Johnson, T.R.; Kauth, P.J.; Stewart, S.L.; Kane, M.E.; Richardson, L. Asymbiotic seed germination, in vitro seedling development, and greenhouse acclimatisation of the threatened terrestrial orchid Bletia purpurea. Plant Cell Tissue Organ Cult. 2008, 94, 11–21. [CrossRef] Parthibhan, S.; Benjamin, J.F.; Muthukumar, M.; Ahamed, N.; Sherif, T.; Senthi Kumar, M.V.R. Influence of nutritional media and photoperiods on in vitro asymbiotic seed germination and seedling development of Dendrobium aqueum Lindley. Afr. J. Plant Sci. 2012, 6, 383–393. [CrossRef] Paul, S.; Kumaria, S.; Tandon, P. An effective nutrient medium for asymbiotic seed germination and large-scale in vitro regeneration of Dendrobium hookerianum, a threatened orchid of northeast India. AoB Plants 2012, 2012, plr032. [CrossRef] Jawan, R.; Gansau, J.A.; Abdullah, J.O. In vitro culture of Borneo’s endemic orchid, Vanda dearei. Asia-Pac. J. Mol. Biol. Biotechnol. 2010, 18, 203–207. Islam, M.; Akter, M.; Prodhan, A. Effect of potato extract on in vitro seed germination and seedling growth of local Vanda roxburgii orchid. J. Bangladesh Agric. Univ. 2012, 9, 211–215. [CrossRef] Dwiyani, R.; Yuswanti, H.; Darmawati, I.A.P.; Suada, K.; Mayadewi, N.N.A. In vitro germination and its subsequent growth of an orchid of Vanda tricolor Lindl. var. suavis from Bali on complex additives enriched medium. J. Agric. Sci. 2015, 37, 144–150. [CrossRef] Jadhav, S.G.; Chavan, D.A.; Waghmare, Y.M. Effect of plant spacing, growth regulator and nutrient management on yield, quality and economics of Bt cotton. J. Cotton Res. Dev. 2015, 29, 48–52. Bhattarai, P. Effects of plant growth regulators on growth and yield of pre-basic seed potato production under glasshouse condition. SAARC J. Agric. 2017, 15, 149–160. [CrossRef] Chin, C.K.; Lee, Z.H.; Mubbarakh, S.A.; Antony, J.J.J.; Chew, B.L.; Subramaniam, S. Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid. Biocatal. Agric. Biotechnol. 2019, 22, 101426. [CrossRef] Hossain, M.M. Asymbiotic seed germination and in vitro seedling development of Epidendrum ibaguense Kunth. (Orchidaceae). Afr. J. Biotechnol. 2008, 7, 3614–3619. Available online: http://www.academicjournals.org/AJB (accessed on 1 September 2019). Mahendran, G.; Muniappan, V.; Ashwini, M.; Muthukumar, T.; Narmatha Bai, V. Asymbiotic seed germination of Cymbidium bicolor Lindl. (Orchidaceae) and the influence of mycorrhizal fungus on seedling development. Acta Physiol. Plant 2013, 35, 829–840. [CrossRef] Roy, A.R.; Patel, R.S.; Patel, V.V.; Sajeev, S.; Deka, B.C. Asymbiotic seed germination, mass propagation and seedling development of Vanda coerulea Griff ex.Lindl. (Blue Vanda): An in vitro protocol for an endangered orchid. Sci. Hortic. 2011, 128, 325–331. [CrossRef] Gallo, F.; Souza, L.; Milaneze, M.; Almeida, O. Seed structure and in vitro seedling development of certain Laeliinae species (Orchidaceae). Rev. Mex. Biodivers. 2016, 87, 68–73. [CrossRef] Gansau, J.A.; Indan, H.; Abdullah, S.N.; David, D.; Marbawi, H.; Jawan, R. Effects of organic additives and plant growth regulators on protocorm development of Dendrobium lowii. Trans. Sci. Technol. 2016, 3, 462–468. Reddy, J.; Niveshika; Shaju, A.; Jose, A.; Betty, A.; Yarmichon, H. Plant growth regulators used for in vitro micropropagation of orchids: A research review. Int. J. Biol. Res. 2021, 8, 37–42. Amiri, S.; Mohammadi, R. Establishment of an efficient in vitro propagation protocol for Sumac (Rhus coriaria L.) and confirmation of the genetic homogeneity. Sci Rep. 2021, 11, 173. [CrossRef] Salsabila, S.S.; Fatimah, K.; Noorhazira, S.; Halimatun, T.S.T.A.B.; Aurifullah, M.; Suhana, Z. Effect of coconut water and peptone in micropropagation of Phalaeonopsis amabilis (L.) Blume Orchid. IOP Conf. Ser. Earth Environ. Sci. 2022, 1102, 012002. [CrossRef] Da Silva, J.A.T.; Chan, M.-T.; Sanjaya; Chai, M.-L.; Tanaka, M. Priming abiotic factors for optimal hybrid Cymbidium (Orchidaceae) PLB and callus induction, plantlet formation, and their subsequent cytogenetic stability analysis. Sci. Hortic. 2006, 109, 368–378. [CrossRef] Kauth, P.J.; Johnson, T.R.; Stewart, S.L.; Kane, M.E. A classroom exercise in hand pollination and in vitro asymbiotic orchid seed germination. Plant Cell Tissue Organ Cult. 2008, 93, 223–230. [CrossRef] Hosomi, S.T.; Santos, R.B.; Custódio, C.C.; Seaton, P.T.; Marx, P.R.; Machado-Neto, N.B. Pre-conditioning Cattleya seeds to improve the efficacy of the tetrazolium test for viability Seed. Seed Sci. Technol. 2011, 39, 178–189. [CrossRef] |
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