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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| Introduction: Goat's milk thought to be a good substitute for cow's milk protein allergic (CMPA) individuals. However, there is growing evidence that their proteins have cross-reactivities with cow's milk allergens. This study aimed to profile and compare milk proteins from different goat breeds that have cross-reactivity to cow's milk allergens. Methodology: Proteomics was used to compare protein extracts of skim milk from Saanen, Jamnapari, and Toggenburg. Cow's milk was used as a control. IgE-immunoblotting and mass spectrometry were used to compare and identify proteins that cross-reacted with serum IgE from CMPA patients (n = 10). Results: The analysis of IgE-reactive proteins revealed that the protein spots identified with high confidence were proteins homologous to common cow's milk allergens such as ?-S1-casein (?S1-CN), ?-casein (?-CN), ?-casein (?-CN), and beta-lactoglobulin (?-LG). Jamnapari's milk proteins were found to cross-react with four major milk allergens: ?-S1-CN, ?-CN, ?-CN, and ?-LG. Saanen goat's milk proteins, on the other hand, cross-reacted with two major milk allergens, ?-S1-CN and ?-LG, whereas Toggenburg goat's milk proteins only react with one of the major milk allergens, ?-CN. Conclusion: These findings may help in the development of hypoallergenic goat milk through cross-breeding strategies of goat breeds with lower allergenic milk protein contents. 2023 Elsevier Ltd |
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