Immunosuppressive effects of annona muricata l. leaf extract on cellular and humoral immune responses in male wistar rats

Mohd Azlan Nafiah

Immunosuppressive effects of annona muricata l. leaf extract on cellular and humoral immune responses in male wistar rats

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General Information

Title

Immunosuppressive effects of annona muricata l. leaf extract on cellular and humoral immune responses in male wistar rats

Type

Article

Main Author

Mohd Azlan Nafiah

Subject

Q Science (General)

ISSN

1389-2010

Language Code

eng

Bibliographic Details

Place of Production

Tanjung Malim

Publisher

Fakulti Sains dan Matematik

Year of publication

2023

Notes

Current Pharmaceutical Biotechnology

Degree Type

Not related

Corporate Name

Universiti Pendidikan Sultan Idris

HTTP Link

Access Web Resource

Abstract

Background: Annona muricata L. (Annonaceae) (AM)'s remarkable anti-inflammatory and anti-cancer activities make it a targeted plant to be explored for its immunomodulatory proper-ties. Traditional practitioners have employed various components of AM to cure a variety of ail-ments, including cancer, diabetes, and inflammation. Objective: The present study evaluated the immunosuppressive effects of 80% ethanol extract of of AM leaves in male Wistar rats on different parameters of humoral and cellular immune responses. Methods: AM leaf extract (AMLE) was analyzed using UHPLC-MS/MS to profile its secondary metabolites. AMLE was rich in polyphenols which include (epi)catechin-(epi)catechin-(epi) cate-chin, caffeic acid, coumaroylquinic acid, hyperin, kaempferol, quinic acid and rutin. The rats were administered 100, 200 and 400 mg/kg bw of the extract daily for 14 days. The effects of AMLE on innate immune responses were determined by evaluating phagocytosis, neutrophils migration, reac-tive oxygen species (ROS) release, CD11b/CD18 integrin expression, and ceruloplasmin, lysozyme and myeloperoxidase (MPO) levels. The adaptive immune parameters were evaluated by immuniz-ing the rats with sheep red blood cells (sRBC) on day 0 and administered orally with AMLE for 14 days. Results: AMLE established significant immunosuppressive effects on the innate immune parameters by inhibiting the neutrophil migration, ROS production, phagocytic activity and expression of CD11b/CD18 integrin in a dose-dependent pattern. AMLE also suppressed ceruloplasmin, MPO and lysozyme expressions in the rat plasma dose-dependently. AMLE dose-dependently inhibited T and B lymphocytes proliferation, Th1 and Th2 cytokine production, CD4+ and CD8+ co-expression in splenocytes, immunoglobulins (IgM and IgG) expression and the sRBC-induced swelling rate of rat paw in delayed-type hypersensitivity (DTH). Conclusion: The strong inhibitory effects on the different parameters of humoral and cellular responses indicate that AMLE has potential to be an important source of effective immuno-suppressive agents. 2023 Bentham Science Publishers.

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