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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| The objective of this study is to develop species-specific environmental DNA (eDNA)
markers for Cichla spp. based on mitochondrial cytochrome oxidase I (COI) gene
sequences. Validation of the developed species-specific eDNA markers were done exsitu
for environmental sample from Tasik Raban. New digital PCR (dPCR) method was
also employed to detect the presence of Cichla ocellaris, and Cichla kelberi in
environmental water samples. Result from this study showed that COI gene can be used
to develop species-specific markers for species Cichla. Ex-situ validation results also
showed that the developed markers CO1 amplified specifically to specimen C. ocellaris
while CK1 amplified directly to specimen of C. kelberi. In addition, dPCR supported
the specificity analysis as absolute fluorescent quantification detected the presence of
C. ocellaris and C. kelberi eDNA in water sample at 90% detection confidence. In
conclusion, CO1 and CK1 markers were successfully developed and specific to be used
as eDNA markers for C. ocellaris and C. kelberi respectively. As an implication, eDNA
analysis with species-specific markers suitable to be used as new rapid monitoring tool
in detection of invasive species for biodiversity management and conservation
purposes. |
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