Fish scale hydroxyapatite/collagen composites incorporated with silver nanoparticles for bone filler applications

Jahil, Mustafa Mudhafar

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Type :	thesis
Subject :	TP Chemical technology
Main Author :	Jahil, Mustafa Mudhafar
Title :	Fish scale hydroxyapatite/collagen composites incorporated with silver nanoparticles for bone filler applications

Place of Production :	Tanjong Malim
Publisher :	Fakulti Sains dan Matematik
Year of Publication :	2021
Notes :	-
Corporate Name :	Perpustakaan Tuanku Bainun
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Abstract : Perpustakaan Tuanku Bainun

The aim of this study was to improve antimicrobial properties of fish scales hydroxyapatite/fish collagen (FsHA/FsCol) composites through infiltration of silver nanoparticles into the matrix for antimicrobial bone filller substitute. In this sudy both hydroxyapatite and collagen were extracted from fish scales using simple hydrothermal extraction method. Silver nanoparticles (AgNPs) were synthesized from silver nitrate using microwave-assisted method in which neems leaves extract was used as reducing agent while fish collagen as stabilizer. FsHA/FsCol/AgNPs composites were prepared in a form of powders and beads using two different methods. Chemical and physical properties of AgNPs and FsHA/FsCol/AgNPs composites were characterized using field emmision scanning electron microscope with energy dispersive x-ray (FESEM-EDX), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and x-ray diffraction technique (XRD). Biological properties of prepared composites were investigated through antibacterial, cytotoxicity and cell attachment tests. The results shown that four different sizes for AgNPs ranging from 28 nm to I 00 nm were obtained by using different concentration of collagen. Their antimicrobial activities against Escherichia coli and Staphylococcus aureus showed that smaller particles sizes of AgNPs have shown better inhibition ofbacteria growth. Analysis using XRD and EDX proved that the pattern of spectra corresponded to silver nanoparticles present in the composites. FESEM-EDX analysis indicated that the silver nanoparticles distributed well in the HA composite matrix. Composites with 80 wt.% of HA and 20 wt.% of collagen demonstrated high stability during degradation test. The addition of 20 wt.% starch in the composite during preparation improved the porosity of composites after sintering, thus improved the infiltration of AgNPs-collagen solution and antibacterial properties. In conclusion, composites ofFsHA/FsCol/AgNPs powder and beads have been proved to be biocompatible and good cell attachment. The implication ofthis study is both powder and beads of FsHA/FsCol/AgNPs composties can be used as antimicrobial bone filler substitute.

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