Molecular approach for identification and validation of Eurycoma longifolia, Labisia pumila, and Orthosiphon stamineus in herbal products

Adibah Abu Bakar

Molecular approach for identification and validation of Eurycoma longifolia, Labisia pumila, and Orthosiphon stamineus in herbal products

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General Information

Title

Molecular approach for identification and validation of Eurycoma longifolia, Labisia pumila, and Orthosiphon stamineus in herbal products

Type

Article

Main Author

Adibah Abu Bakar

Additional Authors

Azi Azeyanty Jamaludin
Al-Obaidi, Jameel R.

Subject

Q Science (General)

ISBN

2314-8535

Language Code

eng

Bibliographic Details

Place of Production

Tanjung Malim

Publisher

Fakulti Sains & Matematik

Year of publication

2024

Notes

Beni-Suef University Journal of Basic and Applied Sciences

Dissertation Note

Other

Degree Type

Others

Corporate Name

Universiti Pendidikan Sultan Idris

HTTP Link

Access Web Resource

Abstract

Background: Eurycoma longifolia (E. longifolia), Labisia pumila (L. pumila), and Orthosiphon stamineus (O. stamineus) are popular species known for their therapeutic properties. An increase in local demand for herbal products makes them susceptible to adulteration, which poses a risk to their safety and efficacy. Current identification methods, such as organoleptic, microscopic, and macroscopic analysis, need to be revised to identify plant species in highly processed herbal products due to their limited ability to detect morphological features and provide comprehensive plant taxonomy information. Methods: This research objective was to develop a simple, reliable, and accurate DNA molecular identification method based on polymerase chain reaction_restriction fragment length polymorphism (PCR_RFLP) for E. longifolia, L. pumila, and O. stamineus, used to validate the species identification for herbal products. PCR_RFLP was developed for rapid identification using restriction enzymes TaqI, BamH I, HinfI, EcoRI, EcoRV, Mbol, and Mspl. Results: The nuclear DNA internal transcribed spacer 2 (ITS2) sequences were identified and compared between plant specimens of E. longifolia, L. pumila, and O. stamineus and 101 samples of commercial herbal products. Plant specimens of E. longifolia, L. pumila, and O. stamineus were successfully identified with high similarity of 100%, 100%, and 99.33%, respectively, based on National Center for Biotechnology Information (NCBI) GenBank. The recovery of DNA sequences from the herbal products was 60.4%, of which 81.97% were identified, and 18.03% showed no sequence through Basic Local Alignment Search Tool (BLAST) identification. Conclusion: A reliable approach for identifying and validating plant species in herbal products has been created using restriction enzymes. This simple and accurate PCR_RFLP approach efficiently identifies E. longifolia, L. pumila, and O. stamineus by analysing ITS2 sequences, assuring consumer health and safety. _ The Author(s) 2024.

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