UPSI Digital Repository (UDRep)
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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| This work aimed to produce silver nanoparticles (AgNPs) by efficient green synthesis techniques, namely rapid green synthesis and modified microwave-assisted green synthesis methods. The study used fish scale collagen (FsCol) as a stabilizer to assess its impact on the dimensions and configurations of AgNPs. Four samples were prepared with varying concentrations of FsCol. The synthesized AgNPs were characterized using Ultraviolet_visible (UV_vis) spectroscopy, scanning electron microscope (SEM), energy dispersive X-ray analysis (EDX), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray diffraction analysis (XRD), Dynamic Light Scattering (DLS), and Transmission electron microscopy (TEM) techniques. The obtained sizes are as follows: 85 _ 15 nm, 70 _ 10 nm, 50 _ 10 nm, and 28_40 nm. The UV_vis spectroscopy revealed a shift in the absorbance peaks from 400 to 446 nm. The SEM method showed a spherical form in all of the samples. The element silver was detected in the EDX examination, along with the presence of oxygen (O) and carbon (C). The FTIR analysis revealed that the peaks seen at 3307 cm_1 were attributed to the stretching of O_H bonds, while the mountain at 1638 cm_1 belonged to the extension of N_H bonds (amide A). Additionally, the band observed at 1638 cm_1 indicated the presence of CO bonds (amide I).The 2140 cm_1 and 1302 cm_1 peaks may be attributed to the C2H2 group present in the plant components and the N_H bending (Amide III), respectively. The XRD pattern indicates that the synthesis process resulted in the formation of crystalline AgNPs. The particle sizes measured using DLS were 121 nm, 96.36 nm, 82.3 nm, and 48.50 nm. The TEM approach revealed that all samples had a spherical morphology with varying sizes: 80_100 nm, 50_80 nm, 40_60 nm, and 28_42 nm. The synthesized AgNPs were tested for their antibacterial properties against the pathogenic pathogens Escherichia coli (E.coli) and Staphylococcus aureus (S. aureus). The influence of AgNPs on bacteria was amplified as the particle size decreased, resulting in a larger inhibitory zone for the smaller particles. _ 2024 The Authors |
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