UPSI Digital Repository (UDRep)
|
UPSI Digital Repository (UDRep)
|
|
|
|
||||||||||||||||||||||||||||
| Abstract : Universiti Pendidikan Sultan Idris |
| Botrytis cinerea, commonly known as gray mold, is a pervasive fungal pathogen that affects a wide range of plant species, leading to significant agricultural losses. The identification of Botrytis cinerea in Malaysia is crucial for protecting the agricultural sector, minimizing economic losses, ensuring food security, maintaining export quality, addressing environmental concerns, and advancing scientific research. In the present research, tomato fruits collected from Cameron Highlands, Pahang, Malaysia showed gray mold disease symptoms of B. cinerea. The fungal isolates were examined morphologically for colony colour, growth rate, conidiophores, conidia shape, and sclerotia on PDA and V8 agar. According to the results, conidiophores appeared in grape shape and length was range of 21.26-32.52 μm, ovoid conidial dimensions were in the range of 10.03-16.08 × 7.37-11.15 μm and sclerotia size was range 1.91-4.50 × 1.70-4.00 mm. All isolates were attributed to the morphospecies Botrytis cinerea on account of these characteristics. The resulting sequences deposited in GenBank were accessions MT012053 to MT012062, respectively. A BLAST analysis of the resulting 550-bp nucleotide sequences showed 99-100% identity closest matched to B. cinerea. The pathogenicity experiments showed P6 isolates of B. cinerea were highly pathogenic and caused gray mold development on tomato fruits that led to severe symptoms in five days. Meanwhile, the least pathogenic isolate was P9. In terms of temperature, B. cinerea grew faster on PDA at 20ºC, slower grew below 20ºC and did not grow at 25ºC. Identification and characterization of B. cinerea on tomato could potentially provide information to assist disease management strategies for B. cinerea. © 2024 Malaysian Society of Applied Biology. |
| References |
Afroz, T., Aktaruzzaman, M. & Kim, B.S. 2019. First report of gray mold on okra caused by Botrytis cinerea in Korea. Plant Disease, 103(5): 1038-1038. https://doi.org/10.1094/PDIS-10-18-1884-PD. NAgrios G.N. 2005. Plant Pathology, 5th Ed. Academic Press, California.Aktaruzzaman, M., Afroz, T., Hong, S.J. & Kim, B.S. 2017. Identification of Botrytis cinerea, the cause of post-harvest gray mold on broccoli in Korea. Research in Plant Disease, 23(4): 372-378. https://doi.org/10.5423/RPD.2017.23.4.372. Aktaruzzaman, M., Afroz, T., Kim, B.S. & Shin, H.D. 2016. First report of gray mold disease of sponge gourd (Luffa cylindrica) caused by Botrytis cinerea in Korea. Research in Plant Disease, 22(2): 107-110. https://doi.org/10.5423/RPD.2016.22.2.107. Aktaruzzaman, M., Kim, J.Y., Xu, S.J. & Kim, B.S. 2014. First report of postharvest gray mold rot on carrot caused by Botrytis cinerea in Korea. Research in Plant Disease, 20(2): 129-131. https://doi.org/10.5423/RPD.2014.20.2.129. Bautista-Baños, S. 2014. Postharvest Decay: Control Strategies. Elsevier. 383 pp. https://doi.org/10.1016/B978-0-12-411552-1.00001-6. Bojkov, G., Mitrev, S. & Arsov, E. 2019. Impact of ampelotechnical measures in the grapevine protection from occurrence of grey mould (Botrytis cinerea). Journal of Animal and Plant Sciences, 17(1): 29-41. Cantu, D., Blanco-Ulate, B., Yang, L., Labavitch, J.M., Bennett, A.B. & Powell, A.L.T. 2009. Ripening-regulated susceptibility of tomato fruit to Botrytis cinerea requires NOR but not RIN or ethylene. Plant Physiology, 150(3): 1434-1449. https://doi.org/10.1104/pp.109.138701. Ciliberti, N., Fermaud, M., Roudet, J. & Rossi, V. 2015. Environmental conditions affect Botrytis cinereainfection of mature grape berries more than the strain or transposon genotype. Phytopathology, 105(8): 1090-1096. https://doi.org/10.1094/PHYTO-10-14-0264-R. Collinge, D. B. & Sarrocco, S. 2022. Transgenic approaches for plant disease control: Status and prospects 2021. Plant Pathology, 71(1): 207-225. https://doi.org/10.1111/ppa.13443. Crisosto, C.H., Garner, D. & Crisosto, G. 2002. Carbon dioxide-enriched atmospheres during cold storage limit losses from Botrytis but accelerate rachis browning of 'Redglobe' table grapes. Postharvest Biology and Technology, 26(2): 181-189. https://doi.org/10.1016/S0925-5214(02)00013-3. Damialis, A., Mohammad, A.B., Halley, J.M. & Gange, A.C. 2015. Fungi in a changing world: Growth rates will be elevated, but spore production may decrease in future climates. International Journal of Biometeorology, 59(9): 1157-1167. https://doi.org/10.1007/s00484-014-0927-0. Derckel, J.P., Baillieul, F., Manteau, S., Audran, J.C., Haye, B., Lambert, B. & Legendre, L. 1999. Differential induction of grapevine defenses by two strains of Botrytis cinerea. Phytopathology, 89(3): 197-203. https://doi.org/10.1094/PHYTO.1999.89.3.197. Elfar, K., Riquelme, D., Zoffoli, J.P. & Latorre, B.A. 2017. First report of Botrytis prunorum causing fruit rot on kiwifruit in Chile. Plant Disease, 101(2): 1-388. https://doi.org/10.1094/PDIS-05-16-0775-PDN. Fillinger, S. & Walker, A.S. 2016. Chemical control and resistance management of Botrytis diseases. In S. Fillinger & Y. Elad (Eds.), Botrytis-The fungus, the pathogen and its management in agricultural systems. Springer. 189-216 pp. https://doi.org/10.1007/978-3-319-23371-0_10. Hegyi-Kaló, J., Holb, I.J., Lengyel, S., Juhász, Á. & Váczy, K.Z. 2019. Effect of year, sampling month and grape cultivar on noble rot incidence, mycelial growth rate and morphological type of Botrytis cinerea during noble rot development. European Journal of Plant Pathology, 155: 339-348. https://doi.org/10.1007/s10658-019-01745-8. Holz, G., Coertze, S. & Williamson, B. 2007. The ecology of Botrytis on plant surface. In Y. Elad; B. Williamson; P. Tudzynski & N. Delen (Eds.), Botrytis: Biology, Pathology and Control. Springer. 9-27 pp. https://doi.org/10.1007/978-1-4020-2626-3_2. Hsiang, T. & Chastagner, G.A. 1992. Production and viability of sclerotia from fungicide-resistant and fungicide-sensitive isolates of Botrytis cinerea, B. elliptica and B. tulipae. Plant Pathology, 41(5): 600-605. https://doi.org/10.1111/j.1365-3059.1992.tb02459.x. Javed, S., Javaid, A., Anwar, W., Majeed, R.A., Akhtar, R. & Naqvi, S.F. 2017. First report of Botrytis bunch rot of grapes caused by Botrytis cinerea in Pakistan. Plant Disease, 101(6): 1036. https://doi.org/10.1094/PDIS-05-16-0762-PDN. Judet-Correia, D., Bollaert, S., Duquenne, A., Charpentier, C., Bensoussan, M. & Dantigny, P. 2010. Validation of a predictive model for the growth of Botrytis cinerea and Penicillium expansum on grape berries. International Journal of Food Microbiology, 142(1-2): 106-113. https://doi.org/10.1016/j.ijfoodmicro.2010.06.009. Khazaeli, P., Zamanizadeh, H., Morid, B. & Bayat, H. 2010. Morphological and molecular identification of Botrytis cinerea causal agent of gray mold in rose greenhouses in central regions of Iran. International Journal of Agricultural Science, 1(1): 19-24. Lalève, A., Fillinger, S. & Walker, A.S. 2014. Fitness measurement reveals contrasting costs in homologous recombinant mutants of Botrytis cinerea resistant to succinate dehydrogenase inhibitors. Fungal Genetics and Biology, 67: 24-36. https://doi.org/10.1016/j.fgb.2014.03.006. Leyronas, C., Duffaud, M., Parès, L., Jeannequin, B. & Nicot, P.C. 2015. Flow of Botrytis cinereainoculum between lettuce crop and soil. Plant Pathology, 64(3): 701-708. https://doi.org/10.1111/ppa.12284. Ma, S., Hu, Y., Liu, S., Sun, J., Irfan, M., Chen, L.J. & Zhang, L. 2018. Isolation, identification and the biological characterization of Botrytis cinerea. International Journal of Agricultural and Biological Engineering, 20(5): 1033-1040. |
| This material may be protected under Copyright Act which governs the making of photocopies or reproductions of copyrighted materials. You may use the digitized material for private study, scholarship, or research. |