Formulation of nanoemulsions from cerbera odollam seeds essential oil and their larvicidal activities against aedes aegypti larvae

Azlan Kamari

Formulation of nanoemulsions from cerbera odollam seeds essential oil and their larvicidal activities against aedes aegypti larvae

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Title

Formulation of nanoemulsions from cerbera odollam seeds essential oil and their larvicidal activities against aedes aegypti larvae

Type

Article

Main Author

Azlan Kamari

Subject

Q Science (General)

ISBN

1823-7010

Language Code

eng

Bibliographic Details

Place of Production

Tanjung Malim

Publisher

Fakulti Sains & Matematik

Year of publication

2024

Notes

Malaysian Journal of Microscopy

Dissertation Note

Other

Degree Type

Others

Corporate Name

Universiti Pendidikan Sultan Idris

HTTP Link

Access Web Resource

Abstract

As of September 2024, there are 322,274 and 94,807 cases due to dengue in Indonesia and Malaysia, respectively. Prompt actions must be taken to control mosquito population and mosquito-borne diseases proliferation. Larvicidal control is one of the effective strategies that are promoted by World Health Organization (WHO). Bintaro (Cerbera odollam) are highly available in wetlands, but they are extremely toxic, especially their seed kernels. In this study, the active compounds of C. odollam seed kernels were gradually extracted using Soxhlet extraction with n-hexane, chloroform and ethanol and were further distilled with a rotary evaporator. Nanoemulsions were formulated with Arabic gum and Triton X-100 by using both homogenisations of high-speed and ultrasonic. Gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared (FTIR) spectroscopy and scanning transmission electron microscopy (STEM) were used to characterise oils and nanoemulsions. Encapsulation efficiencies of nanoemulsions were studied using a UV-Vis spectrophotometer. Larvicidal studies of the nanoemulsions against Aedes aegypti were evaluated for 48 h. The presence of various compounds in different gradual extractions was confirmed by GC-MS chromatograms. The change of intensities and wavenumbers that were presented in FTIR analysis confirmed the successful encapsulation of compounds. Furthermore, spheres with diameters ranged between 8.43 and 55.6 nm were observed in STEM images. The highest encapsulation efficiency (EE) was successfully achieved at 90.3%. Based on log Probit analysis and Abbott’s formula, the LC50 values for extracted compounds ranged between 10.55 and 120.60 mg L-1. The highest mortality rate of larvae was exhibited with extracted compounds from ethanol based-nanoemulsion for 24 h. Overall, these results highlight the usefulness of C. odollam seeds essential oil nanoemulsions in mosquito control and prevention. © Malaysian Journal of Microscopy (2024). All rights reserved.

References

Why is dengue a global issue? (2024). [Online]. [Accessed 5th October 2024]. Available from World WideWeb: https://shorturl.at/TRC07.

World Health Organization global dengue surveillance. (2024). [Online]. [Accessed 5th October 2024]. Available from World Wide Web: https://worldhealthorg.shinyapps. io/dengue_global/.

Messina, J. P., Brady, O. J., Golding, N., Kraemer, M.U., Wint, G.W., Ray, S. E., Pigott, D. M., Shearer, F. M., Johnson, K., Earl, L. & Marczak, L. B. (2019). The current and future global distribution and population at risk of dengue. Nature Microbiology, 4(9), 1508-1515.

Vimercati, G., Britton, R., Evans, T., Measey, J., Mohanty, N. P. & Volery, L. (2024). The Impact of Invasive Alien Vertebrates on Native Insects. In Biological Invasions and Global Insect Decline.Eds. Rodríguez, J., Pyšek, P., & Novoa, A. (Academic Press, London), pp. 189-223.

World Health Organization & UNICEF. (2017). Global Vector Control Response 2017-2030(World Health Organization) pp.1-53.

Nyasembe, V. O. & Torto, B. (2014). Volatile phytochemicals as mosquito semiochemicals. Phytochemistry Letters, 8, 196-201.

Narayanan, M., Vijay, A., Kandasamy, S., Nasif, O., Alharbi, S. A., Srinivasan, R. & Kavitha, R. (2021). Phytochemical profile and larvicidal activity of aqueous extract of Ocimum americanum against mosquito vectors. Applied Nanoscience, 13, 1-13.

Mbatchou, V. C., Tchouassi, D. P., Dickson, R. A., Annan, K., Mensah, A. Y., Amponsah, I. K., Jacob, J.W., Cheseto, X., Habtemariam, S. & Torto, B. (2017). Mosquito larvicidal activity of Cassia tora seed extract and its key anthraquinones aurantio-obtusin and obtusin. Parasites & Vectors, 10, 1-8.

Tarmadi, D., Gunandini, D. J. & Yusuf, S. (2018). Larvicidal Activity of Cerbera odollam Gaertnagainst A Dengue Vector, Aedes aegypti (Diptera: Culicidae). In Sustainable Future for Human Security: Environment and Resources. Ed. McLellan, B. (Springer Nature, Singapore), pp. 175-188.

Meisyara, D., Tarmadi, D., Zulfitri, A., Fajar, A., Ismayati, M., Himmi, S.K., Kartika, T., Guswenrivo, I. and Yusuf, S. (2020). Larvicidal activity of Bintaro (Cerbera odollam) against Culex quinquefasciatus. In IOP Conference Series: Earth and Environmental Science (The 7th Symposium of JAPAN-ASEAN Science Technology Innovation Platform) Tangerang, Indonesia, 24 October 2019.

Aziz, A., Amin, B., Afandi, D., Efriyeldi, E., Gusfa, F., Khayan, K., Masnun, M., Wardoyo, S., Daswito, R. & Dahlan, A. (2024). Toxic effects of bintaro (cerbera manghas) seed extract on aedes aegypti mosquito. Journal of Medicinal and Pharmaceutical Chemistry Research, 6(2),174-182.

Al Jitan, S., Alkhoori, S.A. & Yousef, L.F. (2018). Phenolic Acids from Plants: Extraction and Application to Human Health. In Studies in Natural Products Chemistry,vol. 58. Ed. Atta-ur-Rahman F.R.S. (Elsevier B.V., Amsterdam), pp. 389-417.

Wong, S. T. S., Kamari, A., Abdullah, N. N. A., Yusof, N., Othman, H., Hussein, M. Z. & Hargreaves, J. S. (2024). Physicochemical and morphological characterisation studies of fabrics treated with lavender essential oil-based nanoemulsion. Malaysian Journal of Microscopy, 20(1), 211-223.

Sparkman, O. D., Penton, Z. & Kitson, F. G. (2011). Gas Chromatography and Mass Spectrometry: A Practical Guide. 2nd edition (Academic Press) pp.15-146.

Abidi, N. (2021). FTIR Microspectroscopy: Selected Emerging Applications (Springer International Publishing) pp. 19-23.

Nellist, P.D. (2011). The Principle of STEM Imaging. In Scanning Transmission Electron Microscopy: Imaging and Analysis. Eds.Pennycook, S. J. & Nellist, P. D. (Springer Science & Business Media, New York), pp. 91-116.

Bruschi, M. L. (2015). Mathematical Models of Drug Release. In Strategies to Modify the Drug Release from Pharmaceutical Systems. (Woodhead Publishing, Cambridge) pp. 63-86.

Finney, D. J. (1952). Probit Analysis: A Statistical Treatment of The Sigmoid Response Curve. (Cambridge University Press) pp. 686.

Javed, M.R., Salman, M., Tariq, A., Tawab, A., Zahoor, M. K., Naheed, S., Shahid, M., Ijaz, A. & Ali, H. (2022). The antibacterial and larvicidal potential of bis-(2-ethylhexyl) phthalate from Lactiplantibacillus plantarum. Molecules, 27(21), 7220.