UPSI Digital Repository (UDRep)
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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| The purpose of this study was to investigate the chemical composition and anticholinesterase inhibitory activity of the essential oil obtained from Piper crassipes leaves collected from Malaysia. Twenty-two components were identified using gas chromatography-flame ionisation detection (GC-FID) and gas chromatography/mass spectrometry (GC-MS), which represent 97.8% of the essential oil. The identified major components included chavibetol (59.8%), chavibetol acetate (10.4%), γ-muurolene (5.4%), and germacrene D (4.6%). Anticholinesterase activity was assessed using Ellman’s method. A moderate inhibitory effect was observed for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes with IC50 values of 77.2 ± 0.2 and 89.2 ± 0.2 µg/mL, respectively. The molecular docking studies revealed that chavibetol acetate showed potent interactions with the cholinesterase target, while other compounds exhibited varied binding patterns, providing crucial insights into their potential as cholinesterase inhibitors. © 2024 Informa UK Limited, trading as Taylor & Francis Group. |
| References |
Azman N, Salleh WMNHW, Ghani NA. 2023. Essential oils composition and bioactivities of most prevalent species of genus Piper in Malaysia: a scope review. Riv Ital Sostanze Grasse. 100(2):109–116. Benny A, Thomas J. 2019. Essential oils as treatment strategy for Alzheimer’s disease: current and future perspectives. Planta Med. 85(3):239–248. doi:10.1055/a-0758-0188. Bertrand D, Wallace TL. 2020. A review of the cholinergic system and therapeutic approaches to treat brain disorders. Curr Top Behav Neurosci. 45:1–28. Burkill IH. 1966. A dictionary of the economic product of Malay Peninsula. Kuala Lumpur: Ministry of Agriculture and Co-operatives. Capello TM, Martins EG, de Farias CF, Figueiredo CR, Matsuo AL, Passero LF, Oliveira-Silva D, Sartorelli P, Lago JH. 2015. Chemical composition and in vitro cytotoxic and antileishmanial activities of extract and essential oil from leaves of Piper cernuum. Nat Prod Commun. 10(2):285–288. Cicció JF, Ballestero CM. 1997. Constituyentes volátiles de las hojas y espigas de Piper aduncum (Piperaceae) de Costa Rica. Rev Biol Trop. 45(2):783–790. Ekundayo O, Laakso I, Hiltunen R, Adegbola RM, Oguntimein B, Sofowora A. 1988. Essential oil constituents of ashanti pepper (Piper guineense) fruits (Berries). J Agric Food Chem. 36(5):880–882. doi:10.1021/jf00083a001. Kadir NHA, Salleh WMNHW. 2022. A systematic review on essential oils and biological activities of the genus Syzygium (Myrtaceae). Riv Ital Sostanze Grasse. 99(2):165–178. Kemprai P, Bora PK, Saikia SP, Haldar S. 2023. Chavibetol: major and potent phytotoxin in betel (Piper betle L.) leaf essential oil. Pest Manag Sci. 79(11):4451–4462. doi:10.1002/ps.7645. Minh NP, Nhut VM, Phuong NK. 2019. Microwave vacuum drying behaviour on quality of dried Piper betle leaf. Res Crops. 20(4):815–821. Montalvan M, Malagon O, Cumbicus N, Tanitana F, Gilardoni G. 2023. Chemical analysis of amazonian essential oils of an ecuadorian shuar community. Granja. 38(2):32–44. Morgan RLC, Ho JC. 2009. The antimicrobial activity, mosquito larvicidal activity, antioxidant property and tyrosinase inhibition of Piper betle. J Chinese Chemical Soc. 56(3):653–658. doi:10.1002/jccs.200900097. Mukherjee PK, Kumar V, Mal M, Houghton PJ. 2007. Acetylcholinesterase inhibitors from plants. Phytomedicine. 14(4):289–300. doi:10.1016/j.phymed.2007.02.002. Niculau E, Ribeiro L, Ansante TF, Fernandes JB, Forim MR, Vieira PC, Vendramim JD, Da Silva M. 2018. Isolation of chavibetol and methyleugenol from essential oil of Pimenta pseudocaryophyllus by high performance liquid chromatography. Molecules. 23(11):2909. doi:10.3390/molecules23112909. Panda S, Sharma R, Kar A. 2019. Chavibetol corrects thyrotoxicosis through alterations in thyroid peroxidase. Naunyn Schmiedebergs Arch Pharmacol. 392(5):541–550. doi:10.1007/s00210-018-01606-x. Rimando AM, Han BH, Park JH, Cantoria MC. 1986. Studies on the constituents of Philippine Piper betle leaves. Arch Pharm Res. 9(2):93–97. doi:10.1007/BF02857217. Salazar LC, Ortiz-Reyes A, Rosero DM, Lobo-Echeverri T. 2020. Dillapiole in Piper holtonii as an inhibitor of the symbiotic fungus Leucoagaricus gongylophorus of leaf-cutting ants. J Chem Ecol. 46(8):668–674. doi:10.1007/s10886-020-01170-w. Salleh WMNHW, Abdullah N, Hashim NA, Khong HY, Khamis S, Universiti Pendidikan Sultan Idris. 2019a. Aporphine alkaloids from Piper erecticaule and acetylcholinestearse inhibitory activity. Bol Latinoam Caribe Plant Med Aromat. 18(5):527–532. doi:10.35588/blacpma.19.18.5.35. Salleh WMNHW, Ahmad F, Khong HY. 2014. Chemical compositions and antimicrobial activity of the essential oils of Piper abbreviatum, P. erecticaule and P. lanatum (Piperaceae). Nat Prod Commun. 9(12):1795–1798. Salleh WMNHW, Ahmad F, Khong HY, Sirat HM. 2011. Chemical compositions, antioxidant and antimicrobial activities of the essential oils of Piper caninum Blume. Int J Mol Sci. 12(11):7720–7731. doi:10.3390/ijms12117720. Salleh WMNHW, Ahmad F, Khong HY, Sirat HM. 2012. Chemical compositions and antibacterial activity of the leaf and stem oils of Piper porphyrophyllum. (Excli J. 11:399–406. Salleh WMNHW, Ahmad F, Khong HY, Zulkifli RM. 2016. Essential oil composition of Malaysian Lauraceae: a mini review. Pharm Sci. 22(1):60–67. doi:10.15171/PS.2016.11. Salleh WMNHW, Anuar MZA, Khamis S, Nafiah MA, Sul’ain MD. 2021b. Chemical investigation and biological activities of the essential oil of Knema kunstleri Warb. from Malaysia. Nat Prod Res. 35(13):2279–2284. doi:10.1080/14786419.2019.1669027. Salleh WMNHW, Ghani NA, M, Abdel-Aal AB. 2022. Chemical composition and anticholinesterase activity of Lepisanthes rubiginosa (Roxb.) Leenh. essential oil. Z Naturforsch C J Biosci. 77(11–12):525–529. doi:10.1515/znc-2022-0105. Salleh WMNHW, Hashim NA, Khamis S. 2019b. Chemical constituents and lipoxygenase inhibitory activity of Piper stylosum Miq. Bull Chem Soc Eth. 33(3):587–592. doi:10.4314/bcse. v33i3.19. Salleh WMNHW, Khamis S, Nafiah MA, Abed SA. 2021a. Chemical composition and anticholinesterase inhibitory activity of the essential oil of Pseuduvaria macrophylla (Oliv.) Merr. from Malaysia. Nat Prod Res. 35(11):1887–1892. doi:10.1080/14786419.2019.1639183. Savelev S, Okello E, Perry NS, Wilkins RM, Perry EK. 2003. Synergistic and antagonistic interactions of anticholinesterase terpenoids in Salvia lavandulaefolia essential oil. Pharmacol Biochem Behav. 75(3):661–668. doi:10.1016/s0091-3057(03)00125-4. Schindler B, Heinzmann BM. 2017. Piper gaudichaudianum Kunth: seasonal characterization of the essential oil chemical composition of leaves and reproductive organs. Brazilian Arch Biol Tech. 60:e17160441. Suwanphakdee C, Simpson DA, Hodkinson TR, Chantaranothai P, Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. 2020. A synopsis of Thai Piper (Piperaceae). Thai Forest Bull., Bot. 48(2):145–183. doi:10.20531/tfb.2020.48.2.08. Thinh BB, Chac LD. 2024. Essential oil from the leaves of Fissistigma balansae (A. DC.) Merr.: chemical profile, antimicrobial and anticholinesterase activities. J Essent Oil-Bear Pl. 27(2):574–583. doi:10.1080/0972060X.2024.2324347. Thinh BB, Thin DB. 2023. Essential oil composition, antimicrobial and antioxidant properties of Pluchea eupatorioides Kurz collected from Vietnam. J Essent Oil-Bear Pl. 26(3):653–663. doi:10.1080/0972060X.2023.2234398. Yahaya AAH, Salleh WMNHW. 2022. Magnolia genus - a systematic review on the composition and biological properties of its essential oils. Riv Ital Sostanze Grasse. 99(3):249–261. |
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