UPSI Digital Repository (UDRep)
|
![]() |
|
|
Abstract : Perpustakaan Tuanku Bainun |
This study aims to characterize the molecular and allergenic properties of tropomyosin from local mud crab Scylla species. The methodology involved quantifying reference genes and tropomyosin levels in these crabs using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Additionally, recombinant tropomyosin gene and protein were created using recombinant DNA techniques. The reactivity of tropomyosin with IgE antibodies was assessed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE) and anti-tropomyosin monoclonal antibody immunoblotting. Bioinformatic tools were used to predict various characteristics such as physicochemical properties, structural composition, phylogenetic relationships, IgE-epitope regions, and docking simulations of the recombinant proteins. The findings indicated that elongation factor 1 alpha (EF1_) was a suitable normalizer for qRT-PCR in the three mud crab species studied, and a combination of three reference genes per species was optimal for tropomyosin normalization. Tropomyosin was predominantly present in the abdominal muscle, with S. tranquebarica exhibiting the highest levels, followed by S. olivacea and S. paramamosain. A complete 855-base pair recombinant tropomyosin gene was successfully generated from a local mud crab. The induction with 1 mM IPTG at 37 _C for 4 hours resulted in 42 kDa histidine tagged-recombinant tropomyosin protein from S. olivacea, S. tranquebarica and S. paramamosain that was IgE-reactive to 90%, 65%, and 70% of crab-allergy patients, respectively. Analysis revealed tropomyosin as a temperature-stable coiled-coil alpha-helix protein with 11 IgE-binding sites, leading to cross-reactivity among mud crabs, other crustaceans, insects, arachnids and bacteria. Docking simulations demonstrated binding between these 11 IgE-binding epitopes and IgE antibodies, with distances ranging from 1.2 to 3.6 Angstroms. In conclusion, this study highlights the importance of comprehending the molecular and allergenic aspects of tropomyosin in local mud crab species. These findings have implications for developing diagnostic and therapeutic strategies for mud crab allergies. |
References |
Abdel Rahman, A. M., Kamath, S. D., Lopata, A. L., Robinson, J. J., & Helleur, R. J. (2011). Biomolecular characterization of allergenic proteins in snow crab (Chionoecetes opilio) and de novo sequencing of the second allergen arginine kinase using tandem mass spectrometry. Journal of Proteomics, 74(2), 231-241.
Abdel Rahman, A. M., Lopata, A. L., O’Hehir, R. E., Robinson, J. J., Banoubd, J. H., & Helleur, R. J. (2010). Characterization and de novo sequencing of snow crab tropomyosin enzymatic peptides by both electrospray ionization and matrix- assisted laser desorption ionization QqToF tandem mass spectrometry. Journal of Mass Spectrometry, 45(4), 372-381.
Abramovitch, J. B., Kamath, S., Varese, N., Zubrinich, C., Lopata, A. L., O’Hehir, R. E., et al. (2013). Ige reactivity of blue swimmer crab (Portunus pelagicus) tropomyosin, Por p 1, and other allergens; cross-reactivity with black tiger prawn and effects of heating. PLoS ONE, 8(6), 1-13.
Abramovitch, J. B., Lopata, A. L., O’Hehir, R. E., & Rolland, J. M. (2017). Effect of thermal processing on T cell reactivity of shellfish allergens- discordance with IgE reactivity. PLoS ONE, 12(3), 1-18.
Akay, G., & Yalcinkaya, E. (2019). Skin prick test results to food allergens in patients with allergic rhinitis. Annals of Medical Research, 26, 1024-1048.
Alberts-Hubatsch, H., Lee, S. Y., Meynecke, J. O., Diele, K., Nordhaus, I., & Wolff, M. (2015). Life-history, movement, and habitat use of Scylla serrata (Decapoda, Portunidae): current knowledge and future challenges. Hydrobiologia, 763, 5–21.
Ali, S. A., Dayal, J. S., & Kondusamy, A. (2011). Presentation and evaluation of formulated feed for mud crab Scylla serrata. Indian Journal Fish, 58(2), 67-73.
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403-410.
Amarasekara, G. P., Priyadarshana, T., Manatunge, J., Tanaka, N., & Gunaratne, G. L. (2016). Mud Crab (Scylla serrata) population changes in Koggala Lagoon, Sri Lanka since construction of the groyne system. Aquatic Ecosystem Health and Management, 19(1), 83-91.
Andreson, R., Mos, T., & Remm, M. (2008). Predicting failure rate of PCR in large genomes. Nucleic Acids Research, 36(11), 1-10.
Angamuthu, K., & Piramanayagam, S. (2017). Evaluation of in silico protein secondary structure prediction methods by employing statistical techniques. Biomedical and Biotechnology Research Journal, 1, 29-36.
Asnoussi, A., Aibinu, I. E., Gasser, R. B., Lopata, A. L., & Smooker, P. M. (2017). Molecular and immunological characterisation of tropomyosin from Anisakis pegreffii. Parasitology Research, 116(12), 3291-3301.
Ayuso, R., Lehrer, S. B., & Reese, G. (2002). Identification of continuous, allergenic regions of the major shrimp allergen Pen a 1 (Tropomyosin). International Archives of Allergy and Immunology, 127, 27-37.
Barua, B., Winkelmann, D. A., White, H. D., & Hitchcock-DeGregori, S. E. (2012). Regulation of actin-myosin interaction by conserved periodic sites of tropomyosin. Proceedings of the National Academy of Sciences of the United States of America, 109(45), 18425-18430.
Begum, M., Shah, M. M. R., Mamun, A., & Alam, M. J. (2009). Comparative study of mud crab (Scylla serrata) fattening practices between two different systems in Bangladesh. Journal of the Bangladesh Agricultural University, 7(1), 151-156.
Berumen-Varela, G., Palomino-Hermosillo, Y. A., Bautista-Rosales, P. U., Pena- Sandoval, G. R., Lopez-Guzman, G. G., & Balois-Morales, R. (2020). Identification of reference genes for quantitative real-time PCR in different developmental stages and under refrigeration conditions in soursop fruits (Annona muricata L.). Scientia Horticulturae, 260, 1-6.
Bird, J. A., Lack, G., & Perry, T. T. (2015). Clinical management of food allergy. Journal of Allergy and Clinical Immunology: In Practice, 3(1), 1-11.
Bonlokke, J. H., Gautrin, D., Sigsgaard, T., Lehrer, S. B., Maghni, K., & Cartier, A. (2012). Snow crab allergy and asthma among greenlandic workers- a pilot study. International Journal of Circumpolar Health, 71(1), 1-9.
Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., et al. (2018). Impact of an N‑terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768.
Boye, J. I. (2012). Food allergies in developing and emerging economies: need for comprehensive data on prevalence rates. Clinical and Translational Allergy, 2(25), 1-9.
Bryant, F. R. (1988). Construction of a recombinase-deficient mutant recA protein that retains single-stranded DNA-dependent ATPase activity. Journal of Biological Chemistry, 263(18), 8716-8723.
Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., et al. (2009). The MIQE Guidelines : Minimum information for publication of quantitative real-Time PCR experiments. Clinical Chemistry, 55(2), 611-622.
Cai, Z. L., Chen, J. J., Zhang, Z., Hou, Y. B., He, Y., Sun, J. L., & Ji, K. (2019). Identification of immunodominant IgE binding epitopes of Der p 24, a major allergen of Dermatophagoides pteronyssinus. Clinical and Translational Allergy, 9(28), 1-12.
Cantillo, J. F., Puerta, L., Lafosse-Marin, S., Subiza, J. L., Caraballo, L., & Fernandez- Caldas, E. (2016). Identification and characterization of IgE-binding tropomyosins in Aedes aegypti. International Archives of Allergy and Immunology, 170(1), 46- 56.
Carpenter, J. F., Manning, M. C., & Randolph, T. W. (2002). Long-term storage of proteins. In Current Protocols in Protein Science (pp. 1-6).
Carson, M., Johnson, D. H., McDonald, H., Brouillette, C., & DeLucas, L. J. (2007). His-tag impact on structure. Acta Crystallographica Section D: Biological Crystallography, 63(3), 295-301.
Cartier, A., Lehrer, S. B., Horth-Susin, L., Swanson, M., Neis, B., Howse, D., et al. (2004). Prevalence of crab asthma in crab plant workers in Newfoundland and Labrador. International Journal of Circumpolar Health, 63, 333-336.
Chai, D., Wang, G., Fang, L., Li, H., Liu, S., Zhu, H., et al. (2020). The optimization system for preparation of TG1 competent cells and electrotransformation. MicrobiologyOpen, 9, 1-7.
Chehade, M. (2007). IgE and non-IgE-mediated food allergy: treatment in 2007. Current Opinion in Allergy and Clinical Immunology, 7(3), 264-268.
Chen, H., Yang, H., Wei, J., & Tao, A. (2014). In silico prediction of the T-cell and IgE- binding epitopes of Per a 6 and Bla g 6 allergens in cockroaches. Molecular Medicine Reports, 10(4), 2130-2136.
Chen, X., Lu, L., Xiong, X., Xiong, X., & Liu, Y. (2020). Development of a real-time PCR assay for the identification and quantification of bovine ingredient in processed meat products. Scientific Reports, 10(2052), 1-10.
Davis, C. M., Gupta, R. S., Aktas, O. N., Diaz, V., Kamath, S. D., & Lopata, A. L. (2020). Clinical management of seafood allergy. Journal of Allergy and Clinical Immunology: In Practice, 8(1), 37-44.
de Jonge, H. J. M., Fehrmann, R. S. N., de Bont, E. S. J. M., Hofstra, R. M. W., Gerbens, F., Kamps, W. A., et al. (2007). Evidence based selection of housekeeping genes. PLoS ONE, 2(9), 1-5.
Debode, F., Marien, A., Janssen, E., Bragard, C., & Berben, G. (2017). The influence of amplicon length on real-time PCR results. Biotechnology, Agronomy and Society and Environment, 21(1), 3-11.
Devi, P. L., & Joseph, A. (2015). Taxonomy of mud crabs of the genus Scylla (De Haan) from Cochin Backwaters, Kerala, India. Indian Journal of Fisheries, 62(3), 45–51.
Dixit, S., Jangid, V. K., & Grover, A. (2019). Evaluation of suitable reference genes in Brassica juncea and its wild relative Camelina sativa for qRT-PCR analysis under various stress conditions. PLoS ONE, 14(9), 1-18.
Dooley, S., Seib, T., Engel, M., & Welter, C. (1993). A rapid method to determine the orientation of blunt end ligated polymerase chain reaction products. Electrophoresis, 14(1), 662-663.
Engler, C., Kandzia, R., & Marillonnet, S. (2008). A one pot, one step, precision cloning method with high throughput capability. PLoS ONE, 3(11), 1-7.
Faber, M. A., Pascal, M., El Kharbouchi, O., Sabato, V., Hagendorens, M. M., Decuyper, I. I., et al. (2017). Shellfish allergens: Tropomyosin and beyond. Allergy: European Journal of Allergy and Clinical Immunology, 72(6), 842-848.
Faisal, M., Vasiljevic, T., & Donkor, O. N. (2019). Effects of selected processing treatments on antigenicity of banana prawn (Fenneropenaeus merguiensis) tropomyosin. International Journal of Food Science and Technology, 54(1), 183- 193.
Food and Agriculture Organization. (2018). The state of world fisheries and aquaculture 2018- meeting the sustainable development goals (Retrieved from https://www.fao.org/3/i9540en/I9540EN.pdf).
Food and Agriculture Organization (2019). Global Capture Production 1950-2019 (Retrieved from https://www.fao.org/figis/).
Food and Agriculture Organization. (2020a). Globefish highlights January 2020 issue, with Jan.- Sep. 2019 statistics- A quarterly update on world seafood markets (Retrieved from https://www.fao.org/3/ca7968en/CA7968EN.pdf).
Food and Agriculture Organization, & World Health Organization. (2001). Evaluation of allergenicity of genetically modified foods: report of a joint FAO/WHO expert consultation on allergenicity of foods derived from biotechnology (Retrieved from https://www.fao.org/fileadmin/templates/agns/pdf/topics/ec_jan2001.pdf).
Farah Dayana, Z., Gendeh, B. S., & Salina, H. (2011). Sensitisation to common allergens in children with allergic rhinitis. Brunei International Medical Journal, 7(4), 200-206.
Fazhan, H., Waiho, K., & Ikhwanuddin, M. (2017). Non-indigenous giant mud crab, Scylla serrata (Forskal, 1775) (Crustacea: Brachyura: Portunidae) in Malaysian coastal waters: a call for caution. Marine Biodiversity Records, 10(26), 1-7.
Fazhan, H., Waiho, K., Quinitio, E., Baylon, J. C., Fujaya, Y., Rukminasari, N., et al. (2020). Morphological descriptions and morphometric discriminant function analysis reveal an additional four groups of Scylla spp. PeerJ, 8, 1-19.
Fei, D., Liu, Q., Chen, F., Yang, Y., Chen, Z., Cao, M., & Liu, G. (2016). Assessment of the sensitizing capacity and allergenicity of enzymatic cross-linked arginine kinase, the crab allergen. Molecular Nutrition and Food Research, 60(7), 1707- 1718.
Fernandez-castane, A., Caminal, G., & Lopez-santin, J. (2012). Direct measurements of IPTG enable analysis of the induction behavior of E. coli in high cell density cultures. Microbial Cell Factories, 11(58), 1-9.
Freitas, F. C. P., Depintor, T. S., Agostini, L. T., Luna-Lucena, D., Nunes, F. M. F., Bitondi, M. M. G., et al. (2019). Evaluation of reference genes for gene expression analysis by real-time quantitative PCR (qPCR) in three stingless bee species (Hymenoptera: Apidae: Meliponini). Scientific Reports, 9, 1-13.
Galan-Freyle, N., Olivero-Verbel, J., & Diaz-Lopez, L. (2011). Modeling of allergen proteins found in sea food products. Food Science and Technology, 32(2), 393- 400.
Galli, S. J., & Mindy Tsai. (2016). IgE and mast cells in allergic disease. National Institutes of Health, 18(5), 693-704.
Galli, S. J., Tsai, M., & Piliponsky, A. M. (2008). The development of allergic inflammation. Nature, 454(7203), 1-23.
Gamage, D. G., Gunaratne, A., Periyannan, G. R., & Russell, T. G. (2019). Applicability of instability index for in vitro protein stability prediction. Protein and Peptide Letters, 26(5), 339-347.
Gamez, C., Sanchez-Garcia, S., Ibanez, M. D., Lopez, R., Aguado, E., Lopez, E., et al. (2011). Tropomyosin IgE-positive results are a good predictor of shrimp allergy. Allergy: European Journal of Allergy and Clinical Immunology, 66(10), 1375-1383.
Geciova, J., Bury, D., & Jelen, P. (2002). Methods for disruption of microbial cells for potential use in the dairy industry- A review. International Dairy Journal, 12(6), 541-553.
Gendeh, B. S., Mujahid, S. H., Murad, S., & Rizal, M. (2004). Atopic sensitization of children with rhinitis in Malaysia. Medical Journal of Malaysia, 59(4), 522-529.
Ghafor, I. M. (2020). Crustacean. In Crustacea (pp. 1-18).
Ghosh, S., Rasheedi, S., Rahim, S. S., Banerjee, S., Choudhary, R. K., Chakhaiyar, P., et al. (2004). Method for enhancing solubility of the expressed recombinant proteins in Escherichia coli. BioTechniques, 37(3), 418-423.
Graslund, S., Nordlund, P., Weigelt, J., Hallberg, B. M., Bray, J., Gileadi, O., et al. (2008). Protein production and purification. Nature Methods, 5(2), 135-146.
Green, M. R., & Sambrook, J. (2019). Ligation and ligases. Cold Spring Harbor Protocols, 2019(8), 583-588.
Groetch, M., & Sampson, H. A. (2016). Management of Food Allergy. Pediatric Allergy: Principles and Practice: Third Edition, 62(6), 420-429.
GSL Biotech LLC. (2020). SnapGene | Software for everyday molecular biology. (Retrieved from https://www.snapgene.com/).
Guan, Y., Zhu, Q., Huang, D., Zhao, S., Lo, L. J., & Peng, J. (2015). An equation to estimate the difference between theoretically predicted and SDS PAGE-displayed molecular weights for an acidic peptide. Scientific Reports, 5(13370), 1-11.
Gunning, P., O’Neill, G., & Hardeman, E. (2008). Tropomyosin-based regulation of the actin cytoskeleton in time and space. Physiological Reviews, 88(1), 1-35.
Gunning, P. W., Schevzov, G., Kee, A. J., & Hardeman, E. C. (2005). Tropomyosin isoforms: divining rods for actin cytoskeleton function. Trends in Cell Biology, 15(6), 333-341.
Guo, R., Wang, L., Xiao, P., & Sun, P. (2019). Skin prick testing to identify food allergens in 8393 children and adolescents with asthma in Chongqing, southwest China. Medical Science Monitor, 25, 8221-8229.
Gupta, N., Agarwal, P., Sachdev, A., & Gupta, D. (2019). Allergy testing- an overview. Indian Pediatrics, 56(11), 951-957.
Gupta, R. S., Warren, C. M., Smith, B. M., Blumenstock, J. A., Jiang, L., Davis, M. M., et al. (2019). The public health impact of parent-reported childhood food allergies in the United States. Pediatrics, 144(6), 1-14.
Guzzi, A. F., Maria, M., Amaro, D. S., Fernando, P., Filho, T., & Gabriel, J. E. (2017). Predicting the effects of the single nucleotide polymorphism A122V on CXC chemokine receptor type 1 of Bos taurus (Artiodactyla : Bovidae) cattle by in silico analyses. Biotemas, 30(4), 1-6.
Haidr, M. A., Rosmilah, M., Som, C. S., & Alsailawi, H. (2018). Bioaccumulation of heavy metals in orange mud crab (Scylla olivacea) from Sungai Merbok, Kedah. International Journal of Academic Research, 1(1), 71-72.
Halleux, S. De, Stura, E., Vanderelst, L., Carlier, V., & Jacquemin, M. (2005). Three- dimensional structure and IgE-binding properties of mature fully active Der p 1, a clinically relevant major allergen. Journal Allergy Clinical Immunology, 117(3), 571-576.
Hanahan, D., Jessee, J., & Bloom, F. R. (1991). Plasmid transformation of Escherichia coli and other bacteria. Methods in Enzymology, 204, 63-113.
Hasan, A., & Rosmilah, M. (2019). Effects of food processing on the stability and quality of shellfish allergens. Journal of US-China Medical Science, 16(3), 149-163.
Hasan, A., Rosmilah, M., Keong, B. P., & Haider, M. A. (2019). The effects of thermal and non-thermal treatments on protein profiles of Scylla tranquebarica (purple mud crab). Plant Archives, 19, 813-816.
Hasan, A., Rosmilah, M., & Mustafa, M. (2021). Major and minor allergen IgE reactivity of purple mud crab (Scylla tranquebarica) against a cross-reactive allergen in crustacean and molluscs in patient with a seafood allergy. Research Journal Pharmacy and Technology, 14(1), 239-244
Hasan, A. J., Rosmilah, M., Zailatul Hani, M. Z., Noormalim, A., Faizal. B., Masita, A., et al. (2021). Identification of common and novel major crab allergens in Scylla tranquebarica and the allergen stability in untreated and vinegar-treated Crab. Iranian Journal of Allergy, Asthma, and Immunology, 20(1), 76–87.
He, X., Cheng, Y., Yang, Y., Xie, J., Chu, K., Zhang, Y., et al. (2020). Cloning, expression and comparison of the properties of Scy p 9, a Scylla paramamosain allergen. Food and Function, 11(4), 3006-3019.
Higgins, N. P., & Vologodskii, A. V. (2015). Topological behavior of plasmid DNA. Plasmids, 3(2), 105-131.
Hill, B. J. (1975). Abundance, breeding and growth of the crab Scylla serrata in two South African estuaries. Marine Biology, 32(2), 119-126.
Hoh, K., & Swaminathan, V. (2017). In silico identification and characterization of putative kuruma prawn (Marsupenaeus japonicus) allergens. Indian Journal of Allergy, Asthma and Immunology, 31(2), 87-93.
Hoseini, S. S., & Sauer, M. G. (2015). Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering. Journal of Biological Engineering, 9(1), 1-12.
Hsu, M. L., & Li, L. F. (2012). Prevalence of food avoidance and food allergy in Chinese patients with chronic urticaria. British Journal of Dermatology, 166(4), 747-752.
Hu, M., Liu, G., Yang, Y., Pan, T., Liu, Y., Sun, L., et al. (2017). Cloning, expression, and the effects of processing on sarcoplasmic-calcium-binding protein: an important allergen in mud crab. Journal of Agricultural and Food Chemistry, 65(30), 6247-6257.
Hu, Y., Fu, H., Qiao, H., Sun, S., Zhang, W., Jin, S., et al. (2018). Validation and evaluation of reference genes for quantitative real-Time PCR in Macrobrachium Nipponense. International Journal of Molecular Sciences, 19, 2258-2274.
Huang, S., Chen, X., Wang, J., Chen, J., Yue, W., Lu, W., et al (2017). Selection of appropriate reference genes for qPCR in the Chinese mitten crab, Eriocheir sinensis (Decapoda, Varunidae). Crustaceana, 90(3), 275-296.
Huang, Y. Y., Liu, G. M., Cai, Q. F., Weng, W. Y., Maleki, S. J., Su, W., et al. (2010). Stability of major allergen tropomyosin and other food proteins of mud crab (Scylla serrata) by in vitro gastrointestinal digestion. Food and Chemical Toxicology, 48(5), 1196-1201.
Huang, Z., Feng, W., Wei, W., Yang, B., & Wang, L. (2019). Prevalence of food- allergen and aeroallergen sensitization among people in Sichuan, Western China: an 8-year observational study. Journal of Clinical Laboratory Analysis, 33(3), 1-6.
Huby, R. D. J., Dearman, R. J., & Kimber, I. (2000). Why are some proteins allergens? Toxicological Sciences, 55, 235-246.
Hypersensitivities | Microbiology. (n.d.). (Retrieved from https://courses.lumenlearning.com/suny- microbiology/chapter/hypersensitivities/).
Ihwan, M. Z., Ikhwanuddin, M., Wahida, W., Ambak, M. A., & Marina, H. (2014). Present distribution of pedunculate barnacle Of wild mud crab Scylla Olivacea from Setiu Wetland, Terengganu coastal water, Malaysia. Poultry, Fisheries & Wildlife Sciences, 2(2), 1-3.
Ikai, A. (1980). Thermostability and aliphatic index of globular proteins. Communication, 88, 1895-1898.
Ikhwanuddin, M., Azmie, G., Juariah, H. M., Zakaria, M. Z., & Ambak, M. A. (2011). Biological information and population features of mud crab, genus Scylla from mangrove areas of Sarawak, Malaysia. Fisheries Research, 108(2-3), 299-306.
Ikhwanuddin, M., Azmie, G., Mohd Ihwan, Z., & Abol Munafi, A. B. (2013). Mud crab culture system and practice in Malaysia (pp. 1-31).
Ikhwanuddin, M., Bachok, Z., Hilmi, M. G., Azmie, G., & Zakaria, M. Z. (2010). Species diversity, carapace width-body weight relationship, size distribution and sex ratio of mud crab, genus Scylla from Setiu Wetlands of Terengganu coastal waters, Malaysia. Journal of Sustainability Science and Management, 5(2), 97-109.
Ikhwanuddin, M., Bachok, Z., Mohd Faizal, W. W. Y., Azmie, G., & Abol-Munafi, A. B. (2010). Size of maturity of mud crab Scylla olivacea (Herbst, 1796) from mangrove areas of Terengganu coastal waters. Journal of Sustainability Science and Management, 5(2), 134-147.
Ishikawa, M., Suzuki, F., Ishida, M., Nagashima, Y., & Shiomi, K. (2001). Identification of tropomyosin as a major allergen in the octopus Octopus vulgaris and elucidation of its IgE-binding epitopes. Fisheries Science, 67(5), 934-942.
Ishikawa, M., Ishida, M., Shimakura, K., Nagashima, Y., & Shiomi, K. (1998). Tropomyosin, the major oyster Crassostrea gigas allergen and its IgE-binding epitopes. Journal of Food Science, 63(1), 44-47.
James, J. K., Pike, D. H., Khan, I. J., & Nanda, V. (2018). Structural and dynamic properties of allergen and non-allergen forms of tropomyosin. Structure, 26(7), 997-1006.
Jeong, K. Y. (2004). Characterization of allergenic properties of German cockroach tropomyosin using recombinant proteins. Animal Cells and Systems, 8, 73-73.
Jeong, K. Y., Hong, C., & Yong, T. (2006). Allergenic tropomyosins and their cross- reactivities. Protein and Peptide Letters, 13(8), 835-845.
Jeong, K. Y., Lee, J., Lee, I. Y., Ree, H. I., Hong, C. S., & Yong, T. S. (2003). Allergenicity of recombinant Bla g 7, German cockroach tropomyosin. Allergy: European Journal of Allergy and Clinical Immunology, 58(10), 1059-1063.
Jeong, K. Y., Yum, H., Lee, I., Ree, H., Hong, C., Kim, D. S., et al. (2004). Molecular cloning and characterization of tropomyosin, a major allergen of Chironomus kiiensis, a dominant species of nonbiting midges in Korea. Clinical and Diagnostic Laboratory Immunology, 11(2), 320-324.
Jeong, K. Y., Hwang, H., Lee, J., Lee, I. Y., Kim, D. S., Hong, C. S., et al. (2004). Allergenic characterization of tropomyosin from the dusky brown cockroach, Periplaneta fuliginosa. Clinical and Diagnostic Laboratory Immunology, 11(4), 680-685.
Jeong, K. Y., Lee, H., Lee, J. S., Lee, J., Lee, I. Y., Ree, H. I., et al. (2007). Molecular cloning and the allergenic characterization of tropomyosin from Tyrophagus putrescentiae. Protein Peptide Letters, 14(5), 431-436.
Jeong, K. Y., Han, I. S., Lee, J. Y., Park, K. H., Lee, J. H., & Park, J. W. (2017). Role of tropomyosin in silkworm allergy. Molecular Medicine Reports, 15(5), 3264- 3270.
Jeong, K. Y., Lee, H., Shin, K. H., Yi, M. H., Jeong, K. J., Hong, C. S., et al. (2007). Sequence polymorphisms of major German cockroach allergens Bla g 1, Bla g 2, Bla g 4, and Bla g 5. International Archives of Allergy and Immunology, 145(1), 1- 8.
Jeong, K. Y., Lee, I. Y., Yong, T. S., Lee, J. H., Kim, E. J., Lee, J. S., et al. (2012). Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates. Experimental and Applied Acarology, 58(1), 35-42.
Jeong, K. Y., Lee, J., Lee, I. Y., Ree, H. I., Hong, C. S., & Yong, T. S. (2004). Analysis of amino acid sequence variations and immunoglobulin E-binding epitopes of German cockroach tropomyosin. Clinical and Diagnostic Laboratory Immunology,11(5), 874-878.
Jiang, H., Qian, Z., Lu, W., Ding, H., Yu, H., Wang, H., & Li, J. (2015). Identification and characterization of reference genes for normalizing expression data from red swamp crawfish Procambarus clarkii. International Journal of Molecular Sciences, 16(9), 21591-21605.
Jirapunpipat, K., Aungtonya, C., & Watanabe, S. (2008). Morphological study and application of multivariate analysis for the mud crab Genus Scylla in Klong Ngao Mangrove, Ranong Province, Thailand. Phuket Marine Biological Center Research Bulletin, 24(69), 7-24.
Joseph, B., Furneaux, H., & Illarioshkin, S. N. (1998). Complementary DNA libraries and neurological disease. Archives of Neurology, 55(6), 785-788.
Jutel, M., Solarewicz-Madejek, K., & Smolinska, S. (2012). Recombinant allergens: the present and the future. Human Vaccines and Immunotherapeutics, 8(10), 1534- 1543.
Kamath, S. D., Abdel Rahman, A. M., Komoda, T., & Lopata, A. L. (2013). Impact of heat processing on the detection of the major shellfish allergen tropomyosin in crustaceans and molluscs using specific monoclonal antibodies. Food Chemistry, 141(4), 4031-4039.
Keenan, C. P., Davie, P. J. F., & Mann, D. L. (1998). A revision of the genus Scylla de Haan, 1833 (Crustacea: Decapoda: Brachyura: Portunidae). The Raffles Bulletin of Zoology, 46(1), 217-245.
Keong, B. P., Rosmilah, M., & Som Cit, S. (2019). Optimal preservation and storage regimes of total RNAs from different fish tissues. Pertanika Journal of Tropical Agricultural Science, 42(4), 1289-1294.
Khan, F., Orson, F., Ogawa, Y., Parker, C., & Davis, C. M. (2011). Adult seafood allergy in the Texas Medical Center: a 13-year experience. Allergy & Rhinology, 2(2), 1- 7.
Klinbunga, S., Boonyapakdee, A., & Pratoomchat, B. (2015). Genetic diversity and species-diagnostic markers of mud crabs (Genus Scylla) in Eastern Thailand Determined by RAPD analysis. Marine biotechnology, 2(2), 180-187.
Koeberl, M., Kamath, S. D., Saptarshi, S. R., Smout, M. J., Rolland, J. M., O’Hehir, R. E., et al. (2014). Auto-induction for high yield expression of recombinant novel isoallergen tropomyosin from King prawn (Melicertus latisulcatus) for improved diagnostics and immunotherapeutics. Journal of Immunological Methods, 415, 6-16
Kornienko, E. S. (2020). The reproductive strategy of hermit crabs in temperate waters. Russian Journal of Marine Biology, 46(5), 319-329.
Kostylev, M., Otwell, A. E., Richardson, R. E., & Suzuki, Y. (2015). Cloning should be simple: Escherichia coli DH5α-mediated assembly of multiple DNA fragments with short end homologies. PLoS ONE, 10(9), 1-15.
Kowalski, M. L., Ansotegui, I., Aberer, W., Al-Ahmad, M., Akdis, M., Ballmer-Weber, B. K., et al. (2016). Risk and safety requirements for diagnostic and therapeutic procedures in allergology: World allergy organization statement. World Allergy Organization Journal, 9(1), 1-42.
Krieger, J., Raming, K., Knipper, M., Grau, M., Mertens, S., & Breer, H. (1990). Cloning, sequencing and expression of locust tropomyosin. Insect Biochemistry, 20(2), 173-184.
Kuballa, A. V., Holton, T. A., Paterson, B., & Elizur, A. (2011). Moult cycle specific differential gene expression profiling of the crab Portunus pelagicus. BMC Genomics, 12(1), 147-164.
Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549.
Kumjim, S., Jirapongsananuruk, O., & Piboonpocanun, S. (2016). Cloning and characterization of recombinant tropomyosin of giant freshwater shrimp M. rosenbergii to determine major allergens causing allergic reactions among shrimp-allergic children. Asian Pacific Journal of Allergy and Immunology, 34(3), 229-235.
Kurcinski, M., Badaczewska-Dawid, A., Kolinski, M., Kolinski, A., & Kmiecik, S. (2020). Flexible docking of peptides to proteins using CABS-dock. Protein science: a publication of the Protein Society, 29(1), 211–222.
Larsen, J. M., Bang-Berthelsen, C. H., Qvortrup, K., Sancho, A. I., Hansen, A. H., Andersen, K. I. H., et al. (2020). Production of allergen-specific immunotherapeutic agents for the treatment of food allergy. Critical Reviews in Biotechnology, 40(6), 881-894.
Larsen, J. N., Broge, L., & Jacobi, H. (2016). Allergy immunotherapy: the future of allergy treatment. Drug Discovery Today, 21(1), 26-37.
Lee, H. L., Tang, M. M., Bakhtiar, M. F., Mohamad Yadzir, Z. H., & Johar, A. (2021). Sensitization to local seafood allergens in adult patients with atopic dermatitis in Malaysia. International Archives of Allergy and Immunology, 182(2), 153-157.
Lee, J. S., Park, S. G., Park, H., Seol, W., Lee, S., & Kim, S. (2002). Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex. Biochemical and Biophysical Research Communications, 291(1), 158- 164.
Lee, P. Y., Costumbrado, J., Hsu, C., & Kim, Y. H. (2012). Agarose gel electrophoresis for the separation of DNA fragments. Journal of Visualized Experiments, (62), 1- 5.
Lee, S., Ban, G., Jeong, K., Shin, Y. S., Park, H. S., Lee, S., et al. (2017). A retrospective study of Korean adults with food allergy: Differences in phenotypes and causes. Allergy, Asthma and Immunology Research, 9(6), 534-539.
Lee, S. V., & Bahaman, A. R. (2010). Modified gel preparation for distict DNA fragment analysis in agarose gel electrophoresis. Tropical Biomedicine, 27(2), 351-354.
Leelatanawit, R., Klanchui, A., Uawisetwathana, U., & Karoonuthaisiri, N. (2012). Validation of reference genes for Real-Time PCR of reproductive system in the black tiger shrimp. PLoS ONE, 7(12), 1-10.
Lekshmi, M., Vartak, V. R., & Gudipati, V. (2016). Variation in proximate composition and fatty acid profiles of mud crab meat with regard to sex and body parts. Indian Journal of Fisheries, 63(2), 147-150.
Leung, N. Y. H., Wai, C. Y. Y., Shu, S., Wang, J., Kenny, T. P., Chu, K. H., et al. (2012). Current immunological and molecular biological perspectives on seafood allergy: A comprehensive review. Clinical Reviews in Allergy and Immunology, 46(3), 180- 197.
Leung, P. S. C., Chen, Y. C., Gershwin, M. E., Wong, S. H., Kwan, H. S., & Chu, K. H. (1998). Identification and molecular characterization of Charybdis feriatus tropomyosin, the major crab allergen. Journal of Allergy and Clinical Immunology, 102(5), 847-852.
Leung, P. S. C., Wing, K. C., Duffey, S., Hoi, S. K., Gershwin, M. E., & Ka, H. C. (1996). IgE reactivity against a cross-reactivity allergen in crustacea and mollusca: Evidence for tropomyosin as the common allergen. Journal of Allergy and Clinical Immunology, 98(5), 954-961.
Leung, P. S., Chen, Y. C., Mykles, D. L., Chow, W. K., Li, C. P., & Chu, K. H. (1998). Molecular identification of the lobster muscle protein tropomyosin as a seafood allergen. Molecular Marine Biology and Biotechnology, 7(1), 12-20.
Li, M., Xia, F., Liu, M., He, X., Chen, Y., Bai, T., et al. (2019). Cloning, expression, and epitope identification of myosin light chain 1: an allergen in mud crab. Journal of Agricultural and Food Chemistry, 67(37), 10458-10469.
Li, X., Yang, H., Chen, H., Wu, J., Liu, Y., & Wei, J. (2014). In silico prediction of T and B cell epitopes of Der f 25 in Dermatophagoides farinae. International Journal of Genomics, 2014, 1-10.
Liang, Y., Cao, M., Su, W., Zhang, L., Huang, Y., & Liu, G. (2008). Identification and characterisation of the major allergen of Chinese mitten crab (Eriocheir sinensis). Food Chemistry, 111(4), 998-1003.
Linhart, B., & Valenta, R. (2005). Molecular design of allergy vaccines. Current Opinion in Immunology, 17, 646-655.
Liu, C., Xin, N., Zhai, Y., Jiang, L., Zhai, J., Zhang, Q., et al. (2014). Reference gene selection for quantitative real-time RT-PCR normalization in the half-smooth tongue sole (Cynoglossus semilaevis) at different developmental stages, in various tissue types and on exposure to chemicals. PLoS ONE, 9(3), 1-10.
Liu, G. M., Cao, M. J., Huang, Y. Y., Cai, Q. F., Weng, W. Y., & Su, W. J. (2010). Comparative study of in vitro digestibility of major allergen tropomyosin and other food proteins of Chinese mitten crab (Eriocheir sinensis). Journal of the Science of Food and Agriculture, 90(10), 1614-1620.
Liu, G. M., Li, B., Yu, H. L., Cao, M. J., Cai, Q. F., Lin, J. W., et al. (2012). Induction of mud crab (Scylla paramamosain) tropomyosin and arginine kinase specific hypersensitivity in BALB/c mice. Journal of the Science of Food and Agriculture, 92(2), 232-238.
Liu, H., Chen, R., Zhang, Q., Peng, H., & Wang, K. (2011). Differential gene expression profile from haematopoietic tissue stem cells of red claw crayfish, Cherax quadricarinatus, in response to WSSV infection. Developmental and Comparative Immunology, 35, 716-724.
Liu, M., Liu, G., Yang, Y., Mei, X., Yang, H., Li, Y., et al. (2018). Thermal processing influences the digestibility and immunoreactivity of muscle proteins of Scylla paramamosain. LWT - Food Science and Technology, 98, 559-567.
Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using Real-Time Quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 25, 402-408.
Lopata, A. L., Kleine-Tebbe, J., & Kamath, S. D. (2016). Allergens and molecular diagnostics of shellfish allergy. Allergo Journal, 25(7), 24-32.
Lopez-Matas, M. A., Iraola, V., Moya, R., Vailes, L. D., Pomes, A., Boquete, M., et al. (2015). Cloning and characterization of tropomyosin from the mite Chortoglyphus arcuatus. Molecular Immunology, 68(2), 634-640.
Luo, W., Hu, H., Tang, W., Zou, X., Huang, H., Huang, Z., et al. (2019). Allergen sensitization pattern of allergic adults and children in Southern China: a survey based on real life data. Allergy, Asthma and Clinical Immunology, 15(1), 1-9.
Ma, H., Ma, C., & Ma, L. (2012). Molecular identification of genus Scylla (Decapoda: Portunidae) based on DNA barcoding and polymerase chain reaction. Biochemical Systematics and Ecology, 41, 41-47.
Ma, H., Ma, C., & Ma, L. (2012). High genetic diversity and low differentiation in mud crab (Scylla paramamosain) along the southeastern coast of China revealed by microsatellite markers. The Journal of Experimental Biology, 215(17), 3120-3125.
Ma, H., Ma, C., Ma, L., & Li, X. (2016). Microsatellite Markers in the mud crab (Scylla paramamosain) and their application in population genetics and marker-assited selection. In Microsatellite Markers (pp. 165-183).
Mak, T. W., & Saunders, E. (2006). Allergy and hypersensitivity. In The Immune Response (pp. 923–962).
Mandal, A., Varkey, M., & Pindaniyil, S. (2014). Molecular markers reveal only two mud crab species of Genus Scylla (Brachyura: Portunidae) in Indian Coastal Waters. Biochemical Genetics, 1(52), 338-354.
Mao, H., Cao, M., Maleki, S. J., Cai, Q., Su, W., Yang, Y., et al. (2013). Structural characterization and IgE epitope analysis of arginine kinase from Scylla paramamosain. Molecular Immunology, 56(4), 463-470.
Mao, S., Wu, L., & Shi, W. (2020). Prevalence and distribution patterns of allergens among children with asthma and asthma-like symptoms in Shanghai, China. Respiratory Research, 21(57), 4-11.
Matricardi, P. M., Kleine-Tebbe, J., Hoffmann, H. J., Valenta, R., Hilger, C., Hofmaier, S., et al. (2016). EAACI Molecular Allergology User’s Guide. Pediatric Allergy and Immunology, 27, 1-250.
Matsuo, H., Yokooji, T., & Taogoshi, T. (2015). Common food allergens and their IgE- binding epitopes. Allergology International, 64(4), 332-343.
Mattos, C. P. D., Dantas, F. J. S., Caldeira-de-Araujo, A., & Moraes, M. O. J. (2004). Agarose gel electrophoresis system in the classroom. Biochemistry and Biology Education, 32(4), 254-257.
Mayze, J. (2013). Alive and kicking: Best practice handling of live mud crabs for retailers (Retrieved from https://www.daf.qld.gov.au/).
Mehennaoui, K., Legay, S., Serchi, T., Guerold, F., Giamberini, L., Gutleb, A. C., et al. (2018). Identification of reference genes for RT-qPCR data normalization in Gammarus fossarum (Crustacea Amphipoda). Scientific Reports, 8, 1-8.
Melen, E., Pomes, A., Vailes, L. D., Arruda, L. K., & Chapman, M. D. (1999). Molecular cloning of per a 1 and definition of the cross-reactive Group 1 cockroach allergens. Journal of Allergy and Clinical Immunology, 103, 859-864.
Melo, B., Rezende, L., Machado, P., Gouveia, N., & Levy, R. (2018). Associations of ultra-processed food and drink products with asthma and wheezing among Brazilian adolescents. Pediatric Allergy and Immunology, 29(5), 504-511.
Milian, E., & Diaz, A. M. (2004). Allergy to house dust mites and asthma. Puerto Rico Health Sciences Journal, 23(1), 47-57.
Millier, L., & Green, P. (1991). OSP: A computer program for choosing PCR and DNA sequencing primers. Genome Research, 1(2), 124-128.
Motoyama, K., Suma, Y., Ishizaki, S., Naashima, Y., Lu, Y., Ushio, H., & Shiomi, K. (2008). Identification of tropomyosins as major allergens in Antarctic krill and mantis shrimp and their amino acid sequence characteristics. Marine Biotechnology, 10(6), 709-718.
Motoyama, K., Suma, Y., Ishizaki, S., Nagashima, Y., & Shiomi, K. (2007). Molecular
Kurochkina, N. (2010). Helix-helix interactions and their impact on protein motifs and assemblies. Journal of Theoretical Biology, 264(2), 585-592.
Kurowski, K., & Boxer, R. W. (2008). Food Allergies: detection and management. American Family Physician, 77(12), 1678-1686. cloning of tropomyosins identified as allergens in six species of crustaceans. Journal of Agricultural and Food Chemistry, 55(3), 985-991.
Muhd-Farouk, H., Nurul Huda, A., Abol-Munafi, A. B., Mardhiyyah, M. P., Nurul Hasyima, I., Hidayah, M., et al. (2019). Development of ovarian maturations in orange mud crab, Scylla olivacea (Herbst, 1796) through induction of eyestalk ablation and methyl farnesoate. Arab Journal of Basic and Applied Sciences, 26(1), 171-181.
Munera, M., Contreras, N., Sanchez, A., Sanchez, J., & Emiliani, Y. (2019). In silico analysis of a major allergen from Rattus norvegicus, Rat n 1, and cross-reactivity with domestic pets. F1000Research, 8, 1-18.
Mustafa, N., Rosmilah, M., Zailatul Hani, M. Y., Faizal, B., Noormalin, A., & Hasan, A. (2018). Comparison of protein profiles and allergenicity of different body parts and genders of Scylla paramamosain. International Journal of Science, Environment and Technology, 7(5), 1759-1765.
Nakano, S., Yoshinuma, T., & Yamada, T. (2008). Reactivity of shrimp allergy-related IgE antibodies to krill tropomyosin. International Archives of Allergy and Immunology, 145(3), 175-181.
Naz, S., Desclozeaux, M., Mounsey, K. E., Chaudhry, F. R., & Walton, S. F. (2017). Characterization of Sarcoptes scabiei tropomyosin and paramyosin: immunoreactive allergens in scabies. American Journal of Tropical Medicine and Hygiene, 97(3), 851-860.
Nehete, J. Y., Bhambar, R. S., Narkhede, M. R., & Gawali, S. R. (2013). Natural proteins: Sources, isolation, characterization and applications. Pharmacognosy Reviews, 7(14), 107-117.
Nugraha, R., Kamath, S. D., Johnston, E., Karnaneedi, S., Ruethers, T., & Lopata, A. L. (2019). Conservation analysis of B-cell allergen epitopes to predict clinical cross-reactivity between shellfish and inhalant invertebrate allergens. Frontiers in Immunology, 10, 1-13.
Nurul Ain, M. S., Noor Amalia, Rodrigues, K., Ransangan, J., & Seok-Kian, A. Y. (2016). Genetic diversity of mud crabs, Scylla tranquebarica in Sabah, Malaysia based on Cytochrome C Oxidase (COI) gene sequence. Songklanakarin Journal of Science and Technology, 38(4), 365-372.
Nurul Ain, M. S., Noor Amalia, S. K., Muhammad Ali, S. H., Julian, R., & Seok-Kian, A. Y. (2019). Species diversity and distribution of mud crab in Marudu Bay mangrove forest reserve, Sabah, Malaysia. Borneo Journal of Marine Science and Aquaculture, 3(1), 18-24.
Nurul Izzah, A. R., Rosmilah, M., & Zailatul Hani, M. Y. (2015). Identification of major and minor allergens of mud crab (Scylla Serrata). Medicine and Health, 10(2), 90- 97.
Ogawa, C. Y., Hamasaki, K., Dan, S., Obata, Y., & Kitada, S. (2012). Species composition, reproduction, and body size of mud crabs, Scylla spp., caught in Urado Bay, Japan. Journal of Crustacean Biology, 32(5), 762-768.
Okayama, H. (2012). Functional cDNA expression cloning: pushing it to the limit. Proceedings of the Japan Academy Series B: Physical and Biological Sciences, 88(3), 102-119.
Overton, J. L., & Macintosh, D. J. (2002). Estimated size at sexual maturity for female mud crabs (Genus Scylla) from two sympatric species within Ban Don Bay, Thailand. Journal of Crustacean Biology, 22(4), 790-797.
Owczarzy, R., Tataurov, A. V., Wu, Y., Manthey, J. A., McQuisten, K. A., Almabrazi, H. G., et al. (2008). IDT SciTools: a suite for analysis and design of nucleic acid oligomers. Nucleic Acids Research, 36, 163-169.
Padate, V. P., Rivonker, C. U., & Ail, A. C. (2013). A new record of Scylla olivacea (Decapoda, Bachyura, Prtunidae) from Goa, central west coast of India- A comparative diagnosis. Indian Journal of Marine Sciences, 42(1), 82-89.
Park, H., Lee, J., Park, K. H., Ann, H. W., Jin, M., Choi, S., et al. (2014). A nationwide survey of inhalant allergens sensitization and levels of indoor major allergens in Korea. Allergy, Asthma and Immunology Research, 6(3), 222-227.
Parvin, I., Islam, K. M. I., Hoq, M. E., & Alam, M. S. (2018). Morphometric identification of mud crabs of the Genus Scylla available in Bangladesh waters. Bangladesh Journal of Fisheries, 30(2), 19-206.
Patel, B. Y., & Volcheck, G. W. (2015). Food allergy: Common causes, diagnosis, and treatment. Mayo Clinic Proceedings, 90(10), 1411-1419.
Pekar, J., Ret, D., & Untersmayr, E. (2018). Stability of allergens. Molecular Immunology, 100, 14-20.
Pfaffl, M. W., Tichopad, A., Prgomet, C., & Neuvians, T. P. (2004). Determination of stable housekeeping genes, differentially regulated target genes and sample integrity : BestKeeper-Excel-based tool using pair-wise correlations. Biotechnology Letters, 26, 509-515.
Phinyo, M., Visudtiphole, V., Roytrakul, S., Phaonakrop, N., Jarayabhand, P., & Klinbunga, S. (2013). General and comparative endocrinology characterization and expression of cell division cycle 2 (Cdc2) mRNA and protein during ovarian development of the giant tiger shrimp Penaeus monodon. General and Comparative Endocrinology, 193, 103-111.
Piboonpocanun, S., Malainual, N., Jirapongsananuruk, O., Vichyanond, P., & Thomas, W. R. (2006). Genetic polymorphisms of major house dust mite allergens. Clinical and Experimental Allergy, 36(4), 510-516.
Pittenger, M. F., Kazzaz, J. A., & Helfman, D. M. (1994). Functional properties of non- muscle tropomyosin isoforms. Current Opinion in Cell Biology, 6(1), 96-104.
Pomes, A., Davies, J. M., Gadermaier, G., Hilger, C., Holzhauser, T., Lidholm, J., et al. (2018). WHO/IUIS allergen nomenclature: providing a common language. Molecular Immunology, 100, 3-13.
Poms, R. E., & Anklam, E. (2005). Tracking and tracing for allergen-free food production chains. Frontis, 10, 77-83.
Prasanthi, C., & Ramesh, K. B. (2019). Comparative evaluation of Scylla species from two fishery conservation areas of east coast of Andhra Pradesh, India. International Journal of Bio-Pharma Research, 8(5), 2593-2601.
Quinitio, E. T., Pedro, J. De, & Parado-estepa, F. D. (2007). Ovarian maturation stages of the mud crab Scylla serrata. Aquaculture Research, 38, 1434-1441.
Ramos, J. D. A., Cheong, N., Lee, B. W., & Chua, K. Y. (2001). cDNA cloning and expression of Blo t 11, the Blomia tropicalis allergen homologous to paramyosin. International Archives of Allergy and Immunology, 126(4), 286-293.
Reese, G., Ayuso, R., & Lehrer, S. B. (1999). Tropomyosin: An invertebrate pan- allergen. International Achieves of Allergy and Immunology, 119, 247-258.
Ridgeway, J. A., & Timm, A. E. (2015). Reference gene selection for quantitative real- time PCR normalization in larvae of three species of Grapholitini (Lepidoptera: Tortricidae). PLoS ONE, 10(6), 1-15.
Rolland, J. M., Varese, N. P., Abramovitch, J. B., Anania, J., Nugraha, R., Kamath, S., et al. (2018). Effect of heat processing on IgE reactivity and cross-reactivity of tropomyosin and other allergens of Asia-pacific mollusc species: identification of novel Sydney rock oyster tropomyosin Sac g 1. Molecular Nutrition and Food Research, 62(14), 1-12.
Rombel, I. T., Sykes, K. F., Rayner, S., & Johnston, S. A. (2002). ORF-FINDER: a vector for high-throughput gene identification. Gene, 282, 33-41.
Rosmilah, M., Nurul Izzah, A. R., Zailatul Hani, M. Y., Noormalin, A., Mohd Faizal, B., & Shahnaz, M. (2015). Characterization of major allergens of local mud crab (Scylla serrata). Scientific REsearch Journal, 12(1), 1-10.
Rosmilah, M., Shahnaz, M., Zailatul Hani, M. Y., & Noormalin, A. (2012). Identification of the major allergens of Charybdis feriatus (red crab) and its cross-reactivity with Portunus pelagicus (blue crab). Asian Pacific Journal of Allergy and Immunology, 30(4), 285-293.
Rosmilah, M., Shahnaz, M., Zailatul Hani, M. Y., Noormalin, A., & Normilah, I. (2012). Identification of tropomyosin and arginine kinase as major allergens of Portunus pelagicus (blue swimming crab). Tropical Biomedicine, 29(3), 467-478.
Rouf, M. A., Shahriar, S. I., Sarower, M. G., & Ahsan, M. A. (2016). Taxonomic clarification of mud crab species of Genus Scylla (Brachyura: Portunidae) available in the coastal regions of Bangladesh. Asian Fisheries Science, 29, 124- 136.
Rougeon, F., Kourilsky, P., & Mach, B. (1975). Insertion of a rabbit β-globin gene sequence into an E. coli plasmid. Nucleic Acids Research, 2(12), 2365-2378.
Ruethers, T., Taki, A. C., Johnston, E. B., Nugraha, R., Le, T. T. K., Kalic, T., et al. (2018). Seafood allergy: a comprehensive review of fish and shellfish allergens. Molecular Immunology, 100, 28-57.
Ruhana, H., & Durrah Syazwani, M. (2019). Morphology and genetic diversity of mud crabs (Portunidae: Scylla) from Teluk Sulaman, Sabah, Malaysian Borneo. ASM Science Journal, 12, 1-9.
Saetang, J., Tipmanee, V., & Benjakul, S. (2022). In silico prediction of cross-reactive epitopes of tropomyosin. Molecules, 27(2667), 1-15.
Sampson, H. A. (2003). Anaphylaxis and emergency treatment. Pediatrics, 111, 1601- 1608.
Sampson, H. A., Gerth V. W. R., Bindslev-Jensen, C., Sicherer, S., Teuber, S. S., Burks, A. W., et al. (2012). Standardizing double-blind, placebo-controlled oral food challenges: American Academy of Allergy, Asthma & Immunology-European Academy of Allergy and Clinical Immunology PRACTALL consensus report. Journal of Allergy and Clinical Immunology, 130(6), 1260-1274.
Santos, A. B. R., Chapman, M. D., Aalberse, R. C., Vailes, D., Virginia, P., Ferriani, L., et al. (1999). Cockroach allergens and asthma in Brazil: Identification of tropomyosin as a major allergen with potential cross-reactivity with mite and shrimp allergens. Journal of Allergy and Clinical Immunology, 104(2), 329-337.
Shafique, R. H., Inam, M., Ismail, M., & Chaudhary, F. R. (2013). Group 10 allergens (tropomyosins) from house-dust mites may cause covariation of sensitization to allergens from other invertebrates. Allergy & Rhinology, 3(2), 74-90.
Sharma, G. M., Khuda, S. E., Parker, C. H., Eischeid, A. C., & Pereira, M. (2017). Detection of allergen markers in food: analytical methods. Food and Drug Administration Paper, 6, 65-121.
Sheikh, S. N., & Lazarus, P. (1997). Re-usable DNA template for the polymerase chain reaction (PCR). Nucleic Acids Research, 25(17), 3537-3542.
Shekhar, M. S., Kiruthika, J., & Ponniah, A. G. (2013). Identification and expression analysis of differentially expressed genes from shrimp (Penaeus monodon) in response to low salinity stress. Fish and Shellfish Immunology, 35(6), 1957-1968.
Shen, Y., Cao, M. J., Cai, Q. F., Su, W. J., Yu, H. L., Ruan, W. W., et al. (2011). Purification, cloning, expression and immunological analysis of Scylla serrata arginine kinase, the crab allergen. Journal of the Science of Food and Agriculture, 91(7), 1326-1335.
Sherson, D., Hansen, I., & Sigsgaard, T. (1989). Occupationally related respiratory symptoms in trout‑processing workers. Allergy, 44(5), 336-341.
Shiao, Y. (2003). A new reverse transcription-polymerase chain reaction method for accurate quantification. BMC Biotechnology, 3, 1-12.
Simossis, V. A., & Heringa, J. (2005). PRALINE: A multiple sequence alignment toolbox that integrates homology-extended and secondary structure information. Nucleic Acids Research, 33, 289-294.
Singh, M. B., & Bhalla, P. L. (2003). Hypoallergenic derivatives of major grass pollen allergens for allergy vaccination. Immunology and Cell Iology, 81, 86-91.
Smoldovskaya, O., Feyzkhanova, G., Arefieva, A., Voloshin, S., Ivashkina, O., Reznikov, Y., et al. (2016). Allergen extracts and recombinant proteins: comparison of efficiency of in vitro allergy diagnostics using multiplex assay on a biological microchip. Allergy, Asthma & Clinical Immunology, 12(9), 1-5.
Soegiarto, G., Mai Shihah, A., Damayanti, L. A., Suseno, A., & Effendi, C. (2019). The prevalence of allergic diseases in school children of metropolitan city in Indonesia shows a similar pattern to that of developed countries. Asia Pacific Allergy, 9(2), 1-10.
Song, J., Li, L., Liu, Z., Li, Q., & Ran, P. (2009). Sequence analysis and expression of a cDNA clone encoding tropomyosin in Sinonovacula constricta. Molecular Biology Reports, 36(2), 315-321.
Sookrung, N., Indrawattana, N., Tungtrongchitr, A., Bunnag, C., Tantilipikorn, P., Kwangsri, S., et al. (2009). Allergenicity of native/ recombinant tropomyosin, per a 7, of American Cockroach (CR), Periplaneta americana, among CR Allergic Thais. Asian Pacific Journal of Allergy and Immunology, 27(1), 9-17.
Stanley, P. (2011). Golgi glycosylation. Cold Sring Harbor Perspective Biology, 3, 1- 13.
Stellwagen, N. C., & Stellwagen, E. (2009). Effect of the matrix on DNA electrophoretic mobility. Journal of Chromatography A, 1216(10), 1917-1929.
Steven, A. L., & Huszalina, H. (2022). In silico analysis of maturation and flocculating protein (rbcl) from d unaliella salina in wastewater treatment. Proceedings of Science and Mathematics, 13(2007), 185-195.
Su, X., Lu, L., Li, Y., Zhen, C., Hu, G., Jiang, K., et al. (2020). Reference gene selection for quantitative realtime PCR (qRT-PCR) expression analysis in Galium aparine L. PLoS ONE, 15(2), 1-12.
Sun, B. Q., Zheng, P. Y., Zhang, X. W., Huang, H. M., Chen, D. H., & Zeng, G. Q. (2014). Prevalence of allergen sensitization among patients with allergic diseases in Guangzhou, Southern China: a four-year observational study. Multidisciplinary Respiratory Medicine, 9(2), 1-9.
Sun, X., Zhao, J., Wang, Q., Shi, G., Yang, J., & Ming, L. (2019). Prevalence of allergen sensitization among 15,534 patients with suspected allergic diseases in Henan Province, China. Asian Pacific Journal of Allergy and Immunology, 37(2), 57-64.
Synnergren, J., Giesler, T. L., Adak, S., Tandon, R., Noaksson, K., Lindahl, A., et al. (2007). Differentiating human embryonic stem cells express a unique housekeeping gene signature. Stem Cells, 25, 473-480.
Takeharu, K. (2001). Brief assessment of stock of mud crabs Scylla spp. in Matang Mangrove forest, Malaysia and proposal for resources management. Japan Agricultural Research Quarterly, 35(2), 5-8.
Tajsharghi, H. (2008). Thick and thin filament gene mutations in striated muscle diseases. International Journal of Molecular Sciences, 9, 1259-1275.
Takai, T., & Izuhara, K. (2015). Allergology international allergens in modern society: updated catalogs and future prospects. Allergology International, 64(4), 293-294.
Teng, F., Yu, L., Sun, J., Wang, N., & Cui, Y. (2018). Homology modeling and prediction of B-cell and T-cell epitopes of the house dust mite allergen der f 20. Molecular Medicine Reports, 17(1), 1807-1812.
Thomas, W. R., Smith, W., Hales, B. J., Mills, K. L., & O’Brien, R. M. (2002). Characterization and immunobiology of house dust mite allergens. International Archives of Allergy and Immunology, 129(1), 1-18.
Tokmakov, A. A., Kurotani, A., & Sato, K. (2021). Protein pI and intracellular localization. Frontier in Molecular Biosciences, 8, 1-6.
Tong, W. S., Yuen, A. W. T., Wai, C. Y. Y., Leung, N. Y. H., Chu, K. H., & Leung, P. S. C. (2018). Diagnosis of fish and shellfish allergies. Journal of Asthma and Allergy, 11, 247-260.
Toth, E., Huszaar, K., Bencsura, P., Kulcsar, P. I., Vodicska, B., Nyeste, A., et al. (2014). Restriction enzyme body doubles and PCR cloning: on the general use of Type IIS restriction enzymes for cloning. PLoS ONE, 9(3), 1-12.
Traidl-Hoffmann, C., Jakob, T., & Behrendt, H. (2009). Determinants of allergenicity. Journal of Allergy and Clinical Immunology, 123(3), 558-566.
Tweedie, J. W., & Stowell, K. M. (2005). Quantification of DNA by agarose gel electrophoresis and analysis of the topoisomers of plasmid and M13 DNA following treatment with a restriction endonuclease or DNA topoisomerase I. Biochemistry and Molecular Biology Education, 33(1), 28-33.
Udvardi, M. K., Czechowski, T., & Scheible, W. R. (2008). Eleven golden rules of quantitative RT-PCR. Plant Cell, 20(7), 1736-1737.
Untergasser, A., Nijveen, H., Rao, X., Bisseling, T., Geurts, R., & Leunissen, J. A. M. (2007). Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Research, 35, 71-74.
Valenta, R., & Niederberger, V. (2007). Recombinant allergens for immunotherapy. Journal of Allergy and Clinical Immunology, 119(4), 826-830.
Vandesompele, J., Preter, K. D., Pattyn, F., Poppe, B., Roy, N. V., Paepe, A. D., et al. (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology, 3(7), 1- 12.
Vay, L. L., Ut, V. N., & Jones, D. A. (2001). Seasonal abundance and recruitment in an estuarine population of mud crabs, Scylla paramamosain, in the Mekong Delta, Vietnam. Hydrobiologia, 449, 231-240.
Verhoeckx, K. C. M., Vissers, Y. M., Baumert, J. L., Faludi, R., Feys, M., Flanagan, S., et al. (2015). Food processing and allergenicity. Food and Chemical Toxicology, 80, 223-240.
Vincze, T., Posfai, J., & Roberts, R. J. (2003). NEBcutter: A program to cleave DNA with restriction enzymes. Nucleic Acids Research, 31(13), 3688-3691.
Viswanathan, C., & Raffi, S. M. (2015). The natural diet of the mud crab Scylla olivacea (Herbst, 1896) in Pichavaram mangroves, India. Saudi Journal of Biological Sciences, 22(6), 698-705.
Wachholz, P. A., Dearman, R. J., & Kimber, I. (2005). Detection of allergen-specific IgE antibody responses. Journal of Immunotoxicology, 1(3–4), 189-199.
Waiho, K., Fazhan, H., Baylon, J. C., Norfaizza, W. I. W., & Ikhwanuddin, M. (2016). Use of abdomen looseness as an indicator of sexual maturity in male mud crab Scylla spp. Journal of Shellfish Research, 35(4), 1027-1035.
Waiho, K., Mustaqim, M., Fazhan, H., Wan Norfaizza, W. I., Megat, F. H., & Ikhwanuddin, M. (2015). Mating behaviour of the orange mud crab, Scylla olivacea: The effect of sex ratio and stocking density on mating success. Aquaculture Reports, 2, 50-57.
Wan, K. S., Yang, W., & Wu, W. F. (2010). A survey of serum specific-IgE to common allergens in primary school children of Taipei city. Asian Pacific Journal of Allergy and Immunology, 28(1), 1-6.
Wan Majdiah, W. M., Nurul Khaiza, Y., Suzina, S. A. H., Che Maraina, C. H., & Noor Suryani, M. A. (2016). Food allergen Sensitization among Malaysian rhinitis patients. International Medical Journal, 23(3), 252-255.
Wang, L., Zhang, X., Zhang, J., Fan, W., Lu, M., & Hu, J. (2018). Proteomic analysis and identification of possible allergenic proteins in mature pollen of Populus tomentosa. International Journal of Molecular Sciences, 19(1), 250-265.
Wang, X., Li, L., Xu, F., & Zhang, G. (2011). Tropomyosin is a nice marker gene for phylogenetic analysis of molluscs. Molecular Biology Reports, 38(7), 4589-4593.
Wheatley, L. M., & Togias, A. (2017). Allergic rhinitis. The New England Journal of Medicine, 372(5), 351-372.
Wilkins, M. R., Gasteiger, E., Bairoch, A., Sanchez, J. C., Williams, K. L., Appel, R. D., & Hochstrasser, D. F. (1999). Protein identification and analysis tools in the ExPASy server. In Methods in Molecular Biology, 112, 531-552.
Woo, C. K., & Bahna, S. L. (2011). Not all shellfish “allergy” is allergy! Clinical and Translational Allergy, 1(3), 1-12.
Wu, C. C., Lee, C. H., Tyan, Y. C., Huang, E. S., Yu, W. T., & Yu, H. S. (2019). Identification of pyruvate kinase 2 as a possible crab allergen and analysis of allergenic proteins in crabs consumed in Taiwan. Food Chemistry, 289, 413-418.
Wu, T. C., Tsai, T. C., Huang, C. F., Chang, F. Y., Lin, C. C., Huang, I. F., et al. (2012). Prevalence of food allergy in Taiwan: A questionnaire-based survey. Internal Medicine Journal, 42(12), 1310-1315.
Xia, F., Li, M., Liu, Q., Liu, M., Yang, Y., Cao, M., et al. (2019). Crystal structure analysis and conformational epitope mutation of triosephosphate isomerase, a mud crab allergen. Journal of Agricultural and Food Chemistry, 67(46), 12918- 12926.
Xie, F., Xiao, P., Chen, D., Xu, L., & Zhang, B. (2012). miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs. Plant Molecular Biology, 80(1), 75-84.
Xu, J., Cao, J., Cao, D., Zhao, T., Huang, X., Zhang, P., et al. (2013). Flanking sequence determination and event-specific detection of genetically modified wheat B73-6-1. Acta Biochimica et Biophysica Sinica, 45(5), 416-421.
Xu, L., Zhang, H., Zhang, X., Lin, H., Guo, Y., Yu, C., et al. (2020). Natural shrimp (Litopenaeus vannamei) tropomyosin shows higher allergic properties than recombinant ones as compared through SWATH-MS-based proteomics and immunological response. Journal of Agricultural and Food Chemistry, 68(41), 11553-11567.
Yang, H., Chen, H., Jin, M., Xie, H., He, S., & Wei, J. (2016). Molecular cloning, expression, IgE binding activities and in silico epitope prediction of Per a 9 allergens of the American cockroach. International Journal of Molecular Medicine, 38(6), 1795-1805.
Yang, Y., Cao, M., Alcocer, M., Liu, Q., Fei, D., Mao, H., et al. (2015). Mapping and characterization of antigenic epitopes of arginine kinase of Scylla paramamosain. Molecular Immunology, 65(2), 310-320.
Yang, Y., Hu, M. J., Jin, T. C., Zhang, Y. X., Liu, G. Y., Li, Y. B., et al. (2019). A comprehensive analysis of the allergenicity and IgE epitopes of myosinogen allergens in Scylla paramamosain. Clinical and Experimental Allergy, 49(1), 108- 119.
Yang, Y., Liu, G., Yang, H., Hu, M., Cao, M., Su, W., et al. (2019). Crystal structure determination of Scylla paramamosain arginine kinase, an allergen that may cause cross-reactivity among invertebrates. Food Chemistry, 271, 597-605.
Yang, Z., Zheng, W., Yung, E., Zhong, N., Wong, G. W. K., & Li, J. (2015). Frequency of food group consumption and risk of allergic disease and sensitization in school children in urban and rural China. Clinical and Experimental Allergy, 45(12), 1823- 1832.
Yu, H., Cao, M., Cai, Q., Weng, W., Su, W., & Liu, G. (2011). Effects of different processing methods on digestibility of Scylla paramamosain allergen (tropomyosin). Food and Chemical Toxicology, 49(4), 791-798.
Yu, H., Ruan, W., Cao, M., Cai, Q., Shen, H., & Liu, G. (2013). Identification of physicochemical properties of Scylla paramamosain allergen, arginine kinase. Journal of the Science of Food and Agriculture, 93(2), 245-253.
Yu, K., Gong, J., Huang, C., Huang, H., & Ye, H. (2015). Characterization of CCTα and evaluating its expression in the mud crab Scylla paramamosain when challenged by low temperatures alone and in combination with high and low salinity. Cell Stress and Chaperones, 20(5), 853-864.
Zaffagnini, M., Fermani, S., Costa, A., Lemaire, S. D., & Trost, P. (2013). Plant cytoplasmic GAPDH: redox post-translational modifications and moonlighting properties. Frontiers in Plant Science, 4(450), 1-18.
Zailatul Hani, M. Y., Leecyous, B., & Amelia Suhana, Z. (2020). Isolation and cloning of tropomyosin and arginine kinase from tiger prawn Penaeus monodon and blue swimming crab Portunus trituberculatus. Journal of Science and Mathematics Letters, 8(2), 2019-2021.
Zailatul Hani, M. Y., Rosmilah, M., Faizal, B., Noormalin, A., & Shahnaz, M. (2015). Tropomyosin and actin identified as major allergens of the carpet clam (Paphia textile) and the effect of cooking on their allergenicity. BioMed Research International, 2015, 13-18.
Zailatul Hani, M. Y., Rosmilah, M., Noormalin, A., Faizal, B., Masita, A., & Shahnaz, M. (2010). Identification of IgE-binding proteins of raw and cooked extracts of Loligo edulis (white squid). Southeast Asian Journal of Tropical Medicine and Public Health, 41(3), 653-659.
Zhang, J., & Tao, A. (2015). Antigenicity, immunogenicity, allergenicity. Allergy Bioinformatics, Translational Bioinformatics (pp. 175-c186).
Zhang, Y., Matsuo, H., & Morita, E. (2006). Cross-reactivity among shrimp, crab and scallops in a patient with a seafood allergy. Journal of Dermatology, 33(3), 174- 177.
Zhang, Z., Li, X., Xiao, H., Nowak-Wegrzyn., A., & Z., P. (2020). IgE-binding epitope mapping of tropomyosin allergen (Exo m 1) from Exopalaemon modestus, the freshwater Siberian prawn. Food Chemistry, 309 (1-9).
Zhou, S., Tao, Z., Shen, C., Qian, D., Wang, C., & Zhou, Q. (2018). β-actin gene expression is variable among individuals and not suitable for normalizing mRNA levels in Portunus trituberculatus. Fish and Shellfish Immunology, 81, 338-342.
Zhu, L., She, T., Zhang, Y., Li, S., Xu, Z., Yan, J., et al. (2016). Identification and characterization of ovary development-related protein EJO1 (Eri s 2) from the ovary of Eriocheir sinensis as a new food allergen. Molecular Nutrition & Food Research, 60(10), 2275-2287.
Zuberbier, T., Edenharter, G., Worm, M., Ehlers, I., Reimann, S., Hantke, T., et al. (2004). Prevalence of adverse reactions to food in Germany- a population study. Allergy: European Journal of Allergy and Clinical Immunology, 59(3), 338-345.
Zukiewicz-Sobczak, W. A., Wroblewska, P., Adamczuk, P., & Kopczynski, P. (2013). Causes, symptoms and prevention of food allergy. Postepy Dermatologii Alergologii, 30(2), 113-116.
|
This material may be protected under Copyright Act which governs the making of photocopies or reproductions of copyrighted materials. You may use the digitized material for private study, scholarship, or research. |