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Type :Thesis
Subject :QH Natural history
Main Author :Nur Farah Hani Azemi
Title :Molecular and allergenic characterization of tropomyosin from local mud crab Scylla species
Hits :5
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2024
Corporate Name :Perpustakaan Tuanku Bainun
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Abstract : Perpustakaan Tuanku Bainun
This study aims to characterize the molecular and allergenic properties of tropomyosin from local mud crab Scylla species. The methodology involved quantifying reference genes and tropomyosin levels in these crabs using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Additionally, recombinant tropomyosin gene and protein were created using recombinant DNA techniques. The reactivity of tropomyosin with IgE antibodies was assessed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE) and anti-tropomyosin monoclonal antibody immunoblotting. Bioinformatic tools were used to predict various characteristics such as physicochemical properties, structural composition, phylogenetic relationships, IgE-epitope regions, and docking simulations of the recombinant proteins. The findings indicated that elongation factor 1 alpha (EF1_) was a suitable normalizer for qRT-PCR in the three mud crab species studied, and a combination of three reference genes per species was optimal for tropomyosin normalization. Tropomyosin was predominantly present in the abdominal muscle, with S. tranquebarica exhibiting the highest levels, followed by S. olivacea and S. paramamosain. A complete 855-base pair recombinant tropomyosin gene was successfully generated from a local mud crab. The induction with 1 mM IPTG at 37 _C for 4 hours resulted in 42 kDa histidine tagged-recombinant tropomyosin protein from S. olivacea, S. tranquebarica and S. paramamosain that was IgE-reactive to 90%, 65%, and 70% of crab-allergy patients, respectively. Analysis revealed tropomyosin as a temperature-stable coiled-coil alpha-helix protein with 11 IgE-binding sites, leading to cross-reactivity among mud crabs, other crustaceans, insects, arachnids and bacteria. Docking simulations demonstrated binding between these 11 IgE-binding epitopes and IgE antibodies, with distances ranging from 1.2 to 3.6 Angstroms. In conclusion, this study highlights the importance of comprehending the molecular and allergenic aspects of tropomyosin in local mud crab species. These findings have implications for developing diagnostic and therapeutic strategies for mud crab allergies.

References

Abdel Rahman, A. M., Kamath, S. D., Lopata, A. L., Robinson, J. J., & Helleur, R. J. (2011). 

Biomolecular characterization of allergenic proteins in snow crab (Chionoecetes opilio) and de novo 

sequencing of the second allergen arginine kinase using tandem mass spectrometry. Journal of 

Proteomics, 74(2), 231-241.

 

Abdel Rahman, A. M., Lopata, A. L., O’Hehir, R. E., Robinson, J. J., Banoubd, J. H., & Helleur, R. 

J. (2010). Characterization and de novo sequencing of snow crab tropomyosin enzymatic peptides by 

both electrospray ionization and matrix- assisted laser desorption ionization QqToF tandem mass 

spectrometry. Journal of Mass Spectrometry, 45(4), 372-381.

 

 

Abramovitch, J. B., Kamath, S., Varese, N., Zubrinich, C., Lopata, A. L., O’Hehir, R. E., et al. 

(2013). Ige reactivity of blue swimmer crab (Portunus pelagicus) tropomyosin, Por p 1, and other 

allergens; cross-reactivity with black tiger prawn and effects of heating. PLoS ONE, 8(6), 1-13.

 

 

Abramovitch, J. B., Lopata, A. L., O’Hehir, R. E., & Rolland, J. M. (2017). Effect of thermal 

processing on T cell reactivity of shellfish allergens- discordance with IgE reactivity. PLoS ONE, 

12(3), 1-18.

 

 

Akay, G., & Yalcinkaya, E. (2019). Skin prick test results to food allergens in patients with 

allergic rhinitis. Annals of Medical Research, 26, 1024-1048.

 

 

Alberts-Hubatsch, H., Lee, S. Y., Meynecke, J. O., Diele, K., Nordhaus, I., & Wolff, M. (2015). 

Life-history, movement, and habitat use of Scylla serrata (Decapoda, Portunidae): current knowledge 

and future challenges. Hydrobiologia, 763, 5–21.

 

 

Ali, S. A., Dayal, J. S., & Kondusamy, A. (2011). Presentation and evaluation of formulated feed 

for mud crab Scylla serrata. Indian Journal Fish, 58(2), 67-73.

 

Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment 

search tool. Journal of Molecular Biology, 215(3), 403-410.

 

 

Amarasekara, G. P., Priyadarshana, T., Manatunge, J., Tanaka, N., & Gunaratne, G.

L. (2016). Mud Crab (Scylla serrata) population changes in Koggala Lagoon, Sri Lanka since 

construction of the groyne system. Aquatic Ecosystem Health and

Management, 19(1), 83-91.

 

Andreson, R., Mos, T., & Remm, M. (2008). Predicting failure rate of PCR in large

genomes. Nucleic Acids Research, 36(11), 1-10.

 

 

Angamuthu, K., & Piramanayagam, S. (2017). Evaluation of in silico protein secondary structure 

prediction methods by employing statistical techniques. Biomedical and Biotechnology Research 

Journal, 1, 29-36.

 

 

Asnoussi, A., Aibinu, I. E., Gasser, R. B., Lopata, A. L., & Smooker, P. M. (2017). Molecular and 

immunological characterisation of tropomyosin from Anisakis pegreffii. Parasitology Research, 

116(12), 3291-3301.

 

 

Ayuso, R., Lehrer, S. B., & Reese, G. (2002). Identification of continuous, allergenic regions of 

the major shrimp allergen Pen a 1 (Tropomyosin). International Archives of Allergy and Immunology, 

127, 27-37.

 

 

Barua, B., Winkelmann, D. A., White, H. D., & Hitchcock-DeGregori, S. E. (2012). Regulation of 

actin-myosin interaction by conserved periodic sites of tropomyosin. Proceedings of the National 

Academy of Sciences of the United States of America, 109(45), 18425-18430.

 

Begum, M., Shah, M. M. R., Mamun, A., & Alam, M. J. (2009). Comparative study of mud crab (Scylla 

serrata) fattening practices between two different systems in Bangladesh. Journal of the Bangladesh 

Agricultural University, 7(1), 151-156.

 

 

Berumen-Varela, G., Palomino-Hermosillo, Y. A., Bautista-Rosales, P. U., Pena- Sandoval,  G.  R.,  

Lopez-Guzman,  G.  G.,  &  Balois-Morales,  R.  (2020). Identification of reference genes for 

quantitative real-time PCR in different developmental stages and under refrigeration conditions in 

soursop fruits (Annona muricata L.). Scientia Horticulturae, 260, 1-6.

 

 

Bird, J. A., Lack, G., & Perry, T. T. (2015). Clinical management of food allergy. Journal of 

Allergy and Clinical Immunology: In Practice, 3(1), 1-11.

 

 

Bonlokke, J. H., Gautrin, D., Sigsgaard, T., Lehrer, S. B., Maghni, K., & Cartier, A. (2012). Snow 

crab allergy and asthma among greenlandic workers- a pilot study. International Journal of 

Circumpolar Health, 71(1), 1-9.

 

 

Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., et al. (2018). 

Impact of an N‑terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768.

 

 

Boye, J. I. (2012). Food allergies in developing and emerging economies: need for comprehensive 

data on prevalence rates. Clinical and Translational Allergy,

2(25), 1-9.

 

Bryant, F. R. (1988). Construction of a recombinase-deficient mutant recA protein that retains 

single-stranded DNA-dependent ATPase activity. Journal of Biological Chemistry, 263(18), 8716-8723.

 

Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., et al. (2009). 

The MIQE Guidelines : Minimum information for publication of quantitative real-Time PCR 

experiments. Clinical Chemistry, 55(2), 611-622.

 

 

Cai, Z. L., Chen, J. J., Zhang, Z., Hou, Y. B., He, Y., Sun, J. L., & Ji, K. (2019).

Identification of immunodominant IgE binding epitopes of Der p 24, a major allergen of 

Dermatophagoides pteronyssinus. Clinical and Translational Allergy, 9(28), 1-12.

 

 

Cantillo, J. F., Puerta, L., Lafosse-Marin, S., Subiza, J. L., Caraballo, L., & Fernandez- Caldas, 

E. (2016). Identification and characterization of IgE-binding tropomyosins in Aedes aegypti. 

International Archives of Allergy and Immunology, 170(1), 46- 56.

 

Carpenter, J. F., Manning, M. C., & Randolph, T. W. (2002). Long-term storage of proteins. In 

Current Protocols in Protein Science (pp. 1-6).

 

 

Carson, M., Johnson, D. H., McDonald, H., Brouillette, C., & DeLucas, L. J. (2007). His-tag impact 

on structure. Acta Crystallographica Section D: Biological Crystallography, 63(3), 295-301.

 

 

Cartier, A., Lehrer, S. B., Horth-Susin, L., Swanson, M., Neis, B., Howse, D., et al. (2004). 

Prevalence of crab asthma in crab plant workers in Newfoundland and Labrador. International Journal 

of Circumpolar Health, 63, 333-336.

 

 

Chai, D., Wang, G., Fang, L., Li, H., Liu, S., Zhu, H., et al. (2020). The optimization system for 

preparation of TG1 competent cells and electrotransformation. MicrobiologyOpen, 9, 1-7.

 

Chehade, M. (2007). IgE and non-IgE-mediated food allergy: treatment in 2007.

Current Opinion in Allergy and Clinical Immunology, 7(3), 264-268.

 

Chen, H., Yang, H., Wei, J., & Tao, A. (2014). In silico prediction of the T-cell and IgE- binding 

epitopes of Per a 6 and Bla g 6 allergens in cockroaches. Molecular Medicine Reports, 10(4), 

2130-2136.

 

 

Chen, X., Lu, L., Xiong, X., Xiong, X., & Liu, Y. (2020). Development of a real-time PCR assay for 

the identification and quantification of bovine ingredient in

processed meat products. Scientific Reports, 10(2052), 1-10.

 

Davis, C. M., Gupta, R. S., Aktas, O. N., Diaz, V., Kamath, S. D., & Lopata, A. L. (2020). Clinical 

management of seafood allergy. Journal of Allergy and Clinical Immunology: In Practice, 8(1), 

37-44.

 

de Jonge, H. J. M., Fehrmann, R. S. N., de Bont, E. S. J. M., Hofstra, R. M. W., Gerbens, F., 

Kamps, W. A., et al. (2007). Evidence based selection of housekeeping genes. PLoS ONE, 2(9), 1-5.

 

 

Debode, F., Marien, A., Janssen, E., Bragard, C., & Berben, G. (2017). The influence of amplicon 

length on real-time PCR results. Biotechnology, Agronomy and Society and Environment, 21(1), 3-11.

 

 

Devi, P. L., & Joseph, A. (2015). Taxonomy of mud crabs of the genus Scylla (De Haan) from Cochin 

Backwaters, Kerala, India. Indian Journal of Fisheries, 62(3), 45–51.

 

Dixit, S., Jangid, V. K., & Grover, A. (2019). Evaluation of suitable reference genes in Brassica 

juncea and its wild relative Camelina sativa for qRT-PCR analysis under various stress conditions. 

PLoS ONE, 14(9), 1-18.

 

 

Dooley, S., Seib, T., Engel, M., & Welter, C. (1993). A rapid method to determine the orientation  

of  blunt  end  ligated  polymerase  chain  reaction  products. Electrophoresis, 14(1), 662-663.

 

Engler, C., Kandzia, R., & Marillonnet, S. (2008). A one pot, one step, precision cloning method 

with high throughput capability. PLoS ONE, 3(11), 1-7.

 

 

Faber, M. A., Pascal, M., El Kharbouchi, O., Sabato, V., Hagendorens, M. M., Decuyper, I. I., et 

al. (2017). Shellfish allergens: Tropomyosin and beyond. Allergy: European Journal of Allergy and 

Clinical Immunology, 72(6), 842-848.

 

 

Faisal, M., Vasiljevic, T., & Donkor, O. N. (2019). Effects of selected processing treatments on 

antigenicity of banana prawn (Fenneropenaeus merguiensis) tropomyosin. International Journal of 

Food Science and Technology, 54(1), 183- 193.

 

Food and Agriculture Organization. (2018). The state of world fisheries and aquaculture 2018- 

meeting the sustainable development goals (Retrieved from 

https://www.fao.org/3/i9540en/I9540EN.pdf).

 

 

Food and Agriculture Organization (2019). Global Capture Production 1950-2019

(Retrieved from https://www.fao.org/figis/).

 

Food and Agriculture Organization. (2020a). Globefish highlights January 2020 issue, with Jan.- 

Sep. 2019 statistics- A quarterly update on world seafood markets (Retrieved from 

https://www.fao.org/3/ca7968en/CA7968EN.pdf).

 

Food and Agriculture Organization, & World Health Organization. (2001). Evaluation of allergenicity 

of genetically modified foods: report of a joint FAO/WHO expert consultation on allergenicity of 

foods derived from biotechnology (Retrieved from 

https://www.fao.org/fileadmin/templates/agns/pdf/topics/ec_jan2001.pdf).

 

Farah Dayana, Z., Gendeh, B. S., & Salina, H. (2011). Sensitisation to common allergens in children 

with allergic rhinitis. Brunei International Medical Journal, 7(4), 200-206.

 

 

Fazhan, H., Waiho, K., & Ikhwanuddin, M. (2017). Non-indigenous giant mud crab, Scylla serrata 

(Forskal, 1775) (Crustacea: Brachyura: Portunidae) in Malaysian coastal waters: a call for caution. 

Marine Biodiversity Records, 10(26), 1-7.

 

 

Fazhan, H., Waiho, K., Quinitio, E., Baylon, J. C., Fujaya, Y., Rukminasari, N., et al. (2020). 

Morphological descriptions and morphometric discriminant function analysis reveal an additional 

four groups of Scylla spp. PeerJ, 8, 1-19.

 

 

Fei, D., Liu, Q., Chen, F., Yang, Y., Chen, Z., Cao, M., & Liu, G. (2016). Assessment of the 

sensitizing capacity and allergenicity of enzymatic cross-linked arginine kinase, the crab 

allergen. Molecular Nutrition and Food Research, 60(7), 1707- 1718.

 

Fernandez-castane, A., Caminal, G., & Lopez-santin, J. (2012). Direct measurements of IPTG enable 

analysis of the induction behavior of E. coli in high cell density cultures. Microbial Cell 

Factories, 11(58), 1-9.

 

 

Freitas, F. C. P., Depintor, T. S., Agostini, L. T., Luna-Lucena, D., Nunes, F. M. F., Bitondi, M. 

M. G., et al. (2019). Evaluation of reference genes for gene expression analysis by real-time 

quantitative PCR (qPCR) in three stingless bee species (Hymenoptera: Apidae: Meliponini). 

Scientific Reports, 9, 1-13.

 

 

Galan-Freyle, N., Olivero-Verbel, J., & Diaz-Lopez, L. (2011). Modeling of allergen proteins found 

in sea food products. Food Science and Technology, 32(2), 393- 400.

 

 

Galli, S. J., & Mindy Tsai. (2016). IgE and mast cells in allergic disease. National Institutes of 

Health, 18(5), 693-704.

 

Galli, S. J., Tsai, M., & Piliponsky, A. M. (2008). The development of allergic

inflammation. Nature, 454(7203), 1-23.

 

Gamage, D. G., Gunaratne, A., Periyannan, G. R., & Russell, T. G. (2019). Applicability of 

instability index for in vitro protein stability prediction. Protein and Peptide Letters, 26(5), 

339-347.

 

Gamez, C., Sanchez-Garcia, S., Ibanez, M. D., Lopez, R., Aguado, E., Lopez, E., et al. (2011). 

Tropomyosin IgE-positive results are a good predictor of shrimp allergy. Allergy: European Journal 

of Allergy and Clinical Immunology, 66(10), 1375-1383.

 

Geciova, J., Bury, D., & Jelen, P. (2002). Methods for disruption of microbial cells for potential 

use in the dairy industry- A review. International Dairy Journal, 12(6), 541-553.

 

 

Gendeh, B. S., Mujahid, S. H., Murad, S., & Rizal, M. (2004). Atopic sensitization of children with 

rhinitis in Malaysia. Medical Journal of Malaysia, 59(4), 522-529.

 

Ghafor, I. M. (2020). Crustacean. In Crustacea (pp. 1-18).

 

 

Ghosh, S., Rasheedi, S., Rahim, S. S., Banerjee, S., Choudhary, R. K., Chakhaiyar, P., et al. 

(2004). Method for enhancing solubility of the expressed recombinant proteins in Escherichia coli. 

BioTechniques, 37(3), 418-423.

 

 

Graslund, S., Nordlund, P., Weigelt, J., Hallberg, B. M., Bray, J., Gileadi, O., et al. (2008). 

Protein production and purification. Nature Methods, 5(2), 135-146.

 

 

Green, M. R., & Sambrook, J. (2019). Ligation and ligases. Cold Spring Harbor Protocols, 2019(8), 

583-588.

 

 

Groetch, M., & Sampson, H. A. (2016). Management of Food Allergy. Pediatric Allergy: Principles and 

Practice: Third Edition, 62(6), 420-429.

 

GSL Biotech LLC. (2020). SnapGene | Software for everyday molecular biology. (Retrieved from 

https://www.snapgene.com/).

 

 

Guan, Y., Zhu, Q., Huang, D., Zhao, S., Lo, L. J., & Peng, J. (2015). An equation to estimate the 

difference between theoretically predicted and SDS PAGE-displayed molecular weights for an acidic 

peptide. Scientific Reports, 5(13370), 1-11.

 

 

Gunning, P., O’Neill, G., & Hardeman, E. (2008). Tropomyosin-based regulation of the

actin cytoskeleton in time and space. Physiological Reviews, 88(1), 1-35.

 

Gunning, P. W., Schevzov, G., Kee, A. J., & Hardeman, E. C. (2005). Tropomyosin isoforms: divining 

rods for actin cytoskeleton function. Trends in Cell Biology, 15(6), 333-341.

 

Guo, R., Wang, L., Xiao, P., & Sun, P. (2019). Skin prick testing to identify food allergens in 

8393 children and adolescents with asthma in Chongqing, southwest China. Medical Science Monitor, 

25, 8221-8229.

 

 

Gupta, N., Agarwal, P., Sachdev, A., & Gupta, D. (2019). Allergy testing- an overview.

Indian Pediatrics, 56(11), 951-957.

 

 

Gupta, R. S., Warren, C. M., Smith, B. M., Blumenstock, J. A., Jiang, L., Davis, M. M., et al. 

(2019). The public health impact of parent-reported childhood food allergies in the United States. 

Pediatrics, 144(6), 1-14.

 

Guzzi, A. F., Maria, M., Amaro, D. S., Fernando, P., Filho, T., & Gabriel, J. E. (2017). Predicting 

the effects of the single nucleotide polymorphism A122V on CXC chemokine receptor type 1 of Bos 

taurus (Artiodactyla : Bovidae) cattle by in silico analyses. Biotemas, 30(4), 1-6.

 

Haidr, M. A., Rosmilah, M., Som, C. S., & Alsailawi, H. (2018). Bioaccumulation of heavy metals in 

orange mud crab (Scylla olivacea) from Sungai Merbok, Kedah. International Journal of Academic 

Research, 1(1), 71-72.

 

 

Halleux, S. De, Stura, E., Vanderelst, L., Carlier, V., & Jacquemin, M. (2005). Three- dimensional 

structure and IgE-binding properties of mature fully active Der p 1, a clinically relevant major 

allergen. Journal Allergy Clinical Immunology, 117(3), 571-576.

 

 

Hanahan, D., Jessee, J., & Bloom, F. R. (1991). Plasmid transformation of Escherichia coli and 

other bacteria. Methods in Enzymology, 204, 63-113.

 

Hasan, A., & Rosmilah, M. (2019). Effects of food processing on the stability and quality of 

shellfish allergens. Journal of US-China Medical Science, 16(3), 149-163.

 

 

Hasan, A., Rosmilah, M., Keong, B. P., & Haider, M. A. (2019). The effects of thermal and 

non-thermal treatments on protein profiles of Scylla tranquebarica (purple mud crab). Plant 

Archives, 19, 813-816.

 

 

Hasan, A., Rosmilah, M., & Mustafa, M. (2021). Major and minor allergen IgE reactivity of purple 

mud crab (Scylla tranquebarica) against a cross-reactive allergen in crustacean and molluscs in 

patient with a seafood allergy. Research Journal

Pharmacy and Technology, 14(1), 239-244

 

Hasan, A. J., Rosmilah, M., Zailatul Hani, M. Z., Noormalim, A., Faizal. B., Masita, A., et al. 

(2021). Identification of common and novel major crab allergens in Scylla tranquebarica and the 

allergen stability in untreated and vinegar-treated Crab. Iranian Journal of Allergy, Asthma, and 

Immunology, 20(1), 76–87.

 

He, X., Cheng, Y., Yang, Y., Xie, J., Chu, K., Zhang, Y., et al. (2020). Cloning, expression and 

comparison of the properties of Scy p 9, a Scylla paramamosain allergen. Food and Function, 11(4), 

3006-3019.

 

 

Higgins, N. P., & Vologodskii, A. V. (2015). Topological behavior of plasmid DNA.

Plasmids, 3(2), 105-131.

 

Hill, B. J. (1975). Abundance, breeding and growth of the crab Scylla serrata in two South African 

estuaries. Marine Biology, 32(2), 119-126.

 

 

Hoh, K., & Swaminathan, V. (2017). In silico identification and characterization of putative kuruma 

prawn (Marsupenaeus japonicus) allergens. Indian Journal of Allergy, Asthma and Immunology, 31(2), 

87-93.

 

 

Hoseini, S. S., & Sauer, M. G. (2015). Molecular cloning using polymerase chain reaction, an 

educational guide for cellular engineering. Journal of Biological Engineering, 9(1), 1-12.

 

Hsu, M. L., & Li, L. F. (2012). Prevalence of food avoidance and food allergy in Chinese patients 

with chronic urticaria. British Journal of Dermatology, 166(4), 747-752.

 

Hu, M., Liu, G., Yang, Y., Pan, T., Liu, Y., Sun, L., et al. (2017). Cloning, expression, and the 

effects of processing on sarcoplasmic-calcium-binding protein: an important allergen in mud crab. 

Journal of Agricultural and Food Chemistry, 65(30), 6247-6257.

 

 

Hu, Y., Fu, H., Qiao, H., Sun, S., Zhang, W., Jin, S., et al. (2018). Validation and evaluation of 

reference genes for quantitative real-Time PCR in Macrobrachium Nipponense. International Journal 

of Molecular Sciences, 19, 2258-2274.

 

 

Huang, S., Chen, X., Wang, J., Chen, J., Yue, W., Lu, W., et al (2017). Selection of appropriate 

reference genes for qPCR in the Chinese mitten crab, Eriocheir sinensis (Decapoda, Varunidae). 

Crustaceana, 90(3), 275-296.

 

 

Huang, Y. Y., Liu, G. M., Cai, Q. F., Weng, W. Y., Maleki, S. J., Su, W., et al. (2010). Stability 

of major allergen tropomyosin and other food proteins of mud crab (Scylla serrata) by in vitro 

gastrointestinal digestion. Food and Chemical Toxicology,

48(5), 1196-1201.

 

Huang, Z., Feng, W., Wei, W., Yang, B., & Wang, L. (2019). Prevalence of food- allergen and 

aeroallergen sensitization among people in Sichuan, Western China: an 8-year observational study. 

Journal of Clinical Laboratory Analysis, 33(3), 1-6.

 

 

Huby, R. D. J., Dearman, R. J., & Kimber, I. (2000). Why are some proteins allergens?

Toxicological Sciences, 55, 235-246.

 

 

Hypersensitivities    |    Microbiology.    (n.d.).    (Retrieved    from 

https://courses.lumenlearning.com/suny- microbiology/chapter/hypersensitivities/).

 

 

Ihwan, M. Z., Ikhwanuddin, M., Wahida, W., Ambak, M. A., & Marina, H. (2014). Present distribution 

of pedunculate barnacle Of wild mud crab Scylla Olivacea from Setiu Wetland, Terengganu coastal 

water, Malaysia. Poultry, Fisheries & Wildlife Sciences, 2(2), 1-3.

 

Ikai,  A.  (1980).  Thermostability  and  aliphatic  index  of  globular  proteins.

Communication, 88, 1895-1898.

 

Ikhwanuddin, M., Azmie, G., Juariah, H. M., Zakaria, M. Z., & Ambak, M. A. (2011). Biological 

information and population features of mud crab, genus Scylla from mangrove areas of Sarawak, 

Malaysia. Fisheries Research, 108(2-3), 299-306.

 

 

Ikhwanuddin, M., Azmie, G., Mohd Ihwan, Z., & Abol Munafi, A. B. (2013). Mud crab culture system 

and practice in Malaysia (pp. 1-31).

 

Ikhwanuddin, M., Bachok, Z., Hilmi, M. G., Azmie, G., & Zakaria, M. Z. (2010). Species diversity, 

carapace width-body weight relationship, size distribution and sex ratio of mud crab, genus Scylla 

from Setiu Wetlands of Terengganu coastal waters, Malaysia. Journal of Sustainability Science and 

Management, 5(2), 97-109.

 

 

Ikhwanuddin, M., Bachok, Z., Mohd Faizal, W. W. Y., Azmie, G., & Abol-Munafi, A. B. (2010). Size of 

maturity of mud crab Scylla olivacea (Herbst, 1796) from mangrove areas of Terengganu coastal 

waters. Journal of Sustainability Science and Management, 5(2), 134-147.

 

Ishikawa, M., Suzuki, F., Ishida, M., Nagashima, Y., & Shiomi, K. (2001). Identification of 

tropomyosin as a major allergen in the octopus Octopus vulgaris and elucidation of its IgE-binding 

epitopes. Fisheries Science, 67(5), 934-942.

 

 

Ishikawa, M., Ishida, M., Shimakura, K., Nagashima, Y., & Shiomi, K. (1998). Tropomyosin, the major 

oyster Crassostrea gigas allergen and its IgE-binding

epitopes. Journal of Food Science, 63(1), 44-47.

 

James, J. K., Pike, D. H., Khan, I. J., & Nanda, V. (2018). Structural and dynamic properties of 

allergen and non-allergen forms of tropomyosin. Structure, 26(7), 997-1006.

 

Jeong, K. Y. (2004). Characterization of allergenic properties of German cockroach tropomyosin 

using recombinant proteins. Animal Cells and Systems, 8, 73-73.

 

 

Jeong, K. Y., Hong, C., & Yong, T. (2006). Allergenic tropomyosins and their cross- reactivities. 

Protein and Peptide Letters, 13(8), 835-845.

 

Jeong, K. Y., Lee, J., Lee, I. Y., Ree, H. I., Hong, C. S., & Yong, T. S. (2003).

Allergenicity of recombinant Bla g 7, German cockroach tropomyosin. Allergy: European Journal of 

Allergy and Clinical Immunology, 58(10), 1059-1063.

 

Jeong, K. Y., Yum, H., Lee, I., Ree, H., Hong, C., Kim, D. S., et al. (2004). Molecular cloning and 

characterization of tropomyosin, a major allergen of Chironomus kiiensis, a dominant species of 

nonbiting midges in Korea. Clinical and Diagnostic Laboratory Immunology, 11(2), 320-324.

 

 

Jeong, K. Y., Hwang, H., Lee, J., Lee, I. Y., Kim, D. S., Hong, C. S., et al. (2004). Allergenic 

characterization of tropomyosin from the dusky brown cockroach, Periplaneta fuliginosa. Clinical 

and Diagnostic Laboratory Immunology, 11(4), 680-685.

 

Jeong, K. Y., Lee, H., Lee, J. S., Lee, J., Lee, I. Y., Ree, H. I., et al. (2007). Molecular 

cloning and the allergenic characterization of tropomyosin from Tyrophagus putrescentiae. Protein 

Peptide Letters, 14(5), 431-436.

 

 

Jeong, K. Y., Han, I. S., Lee, J. Y., Park, K. H., Lee, J. H., & Park, J. W. (2017). Role

of tropomyosin in silkworm allergy. Molecular Medicine Reports, 15(5), 3264- 3270.

 

Jeong, K. Y., Lee, H., Shin, K. H., Yi, M. H., Jeong, K. J., Hong, C. S., et al. (2007). Sequence 

polymorphisms of major German cockroach allergens Bla g 1, Bla g 2, Bla g 4, and Bla g 5. 

International Archives of Allergy and Immunology, 145(1), 1- 8.

 

 

Jeong, K. Y., Lee, I. Y., Yong, T. S., Lee, J. H., Kim, E. J., Lee, J. S., et al. (2012).

Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite 

isolates. Experimental and Applied Acarology, 58(1), 35-42.

 

 

Jeong, K. Y., Lee, J., Lee, I. Y., Ree, H. I., Hong, C. S., & Yong, T. S. (2004). Analysis of amino 

acid sequence variations and immunoglobulin E-binding epitopes of

German cockroach tropomyosin. Clinical and Diagnostic Laboratory Immunology,11(5), 874-878.

 

Jiang, H., Qian, Z., Lu, W., Ding, H., Yu, H., Wang, H., & Li, J. (2015). Identification and 

characterization of reference genes for normalizing expression data from red swamp crawfish 

Procambarus clarkii. International Journal of Molecular Sciences, 16(9), 21591-21605.

 

Jirapunpipat, K., Aungtonya, C., & Watanabe, S. (2008). Morphological study and application of 

multivariate analysis for the mud crab Genus Scylla in Klong Ngao Mangrove, Ranong Province, 

Thailand. Phuket Marine Biological Center Research Bulletin, 24(69), 7-24.

 

Joseph, B., Furneaux, H., & Illarioshkin, S. N. (1998). Complementary DNA libraries and 

neurological disease. Archives of Neurology, 55(6), 785-788.

 

 

Jutel, M., Solarewicz-Madejek, K., & Smolinska, S. (2012). Recombinant allergens: the present and 

the future. Human Vaccines and Immunotherapeutics, 8(10), 1534- 1543.

 

 

Kamath, S. D., Abdel Rahman, A. M., Komoda, T., & Lopata, A. L. (2013). Impact of heat processing 

on the detection of the major shellfish allergen tropomyosin in crustaceans and molluscs using 

specific monoclonal antibodies. Food Chemistry, 141(4), 4031-4039.

 

Keenan, C. P., Davie, P. J. F., & Mann, D. L. (1998). A revision of the genus Scylla de Haan, 1833 

(Crustacea: Decapoda: Brachyura: Portunidae). The Raffles Bulletin of Zoology, 46(1), 217-245.

 

 

Keong, B. P., Rosmilah, M., & Som Cit, S. (2019). Optimal preservation and storage regimes of total 

RNAs from different fish tissues. Pertanika Journal of Tropical Agricultural Science, 42(4), 

1289-1294.

 

Khan, F., Orson, F., Ogawa, Y., Parker, C., & Davis, C. M. (2011). Adult seafood allergy in the 

Texas Medical Center: a 13-year experience. Allergy & Rhinology, 2(2), 1- 7.

 

Klinbunga, S., Boonyapakdee, A., & Pratoomchat, B. (2015). Genetic diversity and species-diagnostic 

markers of mud crabs (Genus Scylla) in Eastern Thailand Determined by RAPD analysis. Marine 

biotechnology, 2(2), 180-187.

 

 

Koeberl, M., Kamath, S. D., Saptarshi, S. R., Smout, M. J., Rolland, J. M., O’Hehir, R. E., et al. 

(2014). Auto-induction for high yield expression of recombinant novel isoallergen tropomyosin from 

King prawn (Melicertus latisulcatus) for improved

diagnostics and immunotherapeutics. Journal of Immunological Methods, 415, 6-16

 

Kornienko, E. S. (2020). The reproductive strategy of hermit crabs in temperate waters.

Russian Journal of Marine Biology, 46(5), 319-329.

 

Kostylev, M., Otwell, A. E., Richardson, R. E., & Suzuki, Y. (2015). Cloning should be simple: 

Escherichia coli DH5α-mediated assembly of multiple DNA fragments with short end homologies. PLoS 

ONE, 10(9), 1-15.

 

 

Kowalski, M. L., Ansotegui, I., Aberer, W., Al-Ahmad, M., Akdis, M., Ballmer-Weber, B. K., et al. 

(2016). Risk and safety requirements for diagnostic and therapeutic procedures in allergology: 

World allergy organization statement. World Allergy Organization Journal, 9(1), 1-42.

 

Krieger, J., Raming, K., Knipper, M., Grau, M., Mertens, S., & Breer, H. (1990). Cloning, 

sequencing and expression of locust tropomyosin. Insect Biochemistry, 20(2), 173-184.

 

 

Kuballa, A. V., Holton, T. A., Paterson, B., & Elizur, A. (2011). Moult cycle specific differential 

gene expression profiling of the crab Portunus pelagicus. BMC Genomics, 12(1), 147-164.

 

 

Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular evolutionary 

genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549.

 

 

Kumjim, S., Jirapongsananuruk, O., & Piboonpocanun, S. (2016). Cloning and characterization of 

recombinant tropomyosin of giant freshwater shrimp M. rosenbergii to determine major allergens 

causing allergic reactions among shrimp-allergic children. Asian Pacific Journal of Allergy and 

Immunology, 34(3), 229-235.

 

 

Kurcinski, M., Badaczewska-Dawid, A., Kolinski, M., Kolinski, A., & Kmiecik, S. (2020). Flexible 

docking of peptides to proteins using CABS-dock. Protein science: a publication of the Protein 

Society, 29(1), 211–222.

 

Larsen, J. M., Bang-Berthelsen, C. H., Qvortrup, K., Sancho, A. I., Hansen, A. H., Andersen,  K.  

I.  H.,  et  al.  (2020).  Production  of  allergen-specific immunotherapeutic agents for the 

treatment of food allergy. Critical Reviews in Biotechnology, 40(6), 881-894.

 

Larsen, J. N., Broge, L., & Jacobi, H. (2016). Allergy immunotherapy: the future of allergy 

treatment. Drug Discovery Today, 21(1), 26-37.

 

 

Lee, H. L., Tang, M. M., Bakhtiar, M. F., Mohamad Yadzir, Z. H., & Johar, A. (2021). Sensitization 

to local seafood allergens in adult patients with atopic dermatitis in Malaysia. International 

Archives of Allergy and Immunology, 182(2), 153-157.

 

 

Lee, J. S., Park, S. G., Park, H., Seol, W., Lee, S., & Kim, S. (2002). Interaction network of 

human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex. Biochemical and 

Biophysical Research Communications, 291(1), 158- 164.

 

 

Lee, P. Y., Costumbrado, J., Hsu, C., & Kim, Y. H. (2012). Agarose gel electrophoresis for the 

separation of DNA fragments. Journal of Visualized Experiments, (62), 1- 5.

 

 

Lee, S., Ban, G., Jeong, K., Shin, Y. S., Park, H. S., Lee, S., et al. (2017). A retrospective 

study of Korean adults with food allergy: Differences in phenotypes and causes. Allergy, Asthma and 

Immunology Research, 9(6), 534-539.

 

 

Lee, S. V., & Bahaman, A. R. (2010). Modified gel preparation for distict DNA fragment analysis in 

agarose gel electrophoresis. Tropical Biomedicine, 27(2), 351-354.

 

Leelatanawit, R., Klanchui, A., Uawisetwathana, U., & Karoonuthaisiri, N. (2012). Validation of 

reference genes for Real-Time PCR of reproductive system in the black tiger shrimp. PLoS ONE, 

7(12), 1-10.

 

 

Lekshmi, M., Vartak, V. R., & Gudipati, V. (2016). Variation in proximate composition and fatty 

acid profiles of mud crab meat with regard to sex and body parts. Indian Journal of Fisheries, 

63(2), 147-150.

 

 

Leung, N. Y. H., Wai, C. Y. Y., Shu, S., Wang, J., Kenny, T. P., Chu, K. H., et al. (2012).

Current immunological and molecular biological perspectives on seafood allergy: A comprehensive 

review. Clinical Reviews in Allergy and Immunology, 46(3), 180- 197.

 

 

Leung, P. S. C., Chen, Y. C., Gershwin, M. E., Wong, S. H., Kwan, H. S., & Chu, K. H. (1998). 

Identification and molecular characterization of Charybdis feriatus

tropomyosin, the major crab allergen. Journal of Allergy and Clinical Immunology,

102(5), 847-852.

 

Leung, P. S. C., Wing, K. C., Duffey, S., Hoi, S. K., Gershwin, M. E., & Ka, H. C. (1996). IgE 

reactivity against a cross-reactivity allergen in crustacea and mollusca: Evidence for tropomyosin 

as the common allergen. Journal of Allergy and Clinical Immunology, 98(5), 954-961.

 

 

Leung, P. S., Chen, Y. C., Mykles, D. L., Chow, W. K., Li, C. P., & Chu, K. H. (1998).

Molecular identification of the lobster muscle protein tropomyosin as a seafood allergen. Molecular 

Marine Biology and Biotechnology, 7(1), 12-20.

 

 

Li, M., Xia, F., Liu, M., He, X., Chen, Y., Bai, T., et al. (2019). Cloning, expression, and 

epitope identification of myosin light chain 1: an allergen in mud crab. Journal of Agricultural 

and Food Chemistry, 67(37), 10458-10469.

 

 

Li, X., Yang, H., Chen, H., Wu, J., Liu, Y., & Wei, J. (2014). In silico prediction of T and B cell 

epitopes of Der f 25 in Dermatophagoides farinae. International Journal of Genomics, 2014, 1-10.

 

 

Liang, Y., Cao, M., Su, W., Zhang, L., Huang, Y., & Liu, G. (2008). Identification and 

characterisation of the major allergen of Chinese mitten crab (Eriocheir sinensis). Food Chemistry, 

111(4), 998-1003.

 

Linhart, B., & Valenta, R. (2005). Molecular design of allergy vaccines. Current Opinion in 

Immunology, 17, 646-655.

 

Liu, C., Xin, N., Zhai, Y., Jiang, L., Zhai, J., Zhang, Q., et al. (2014). Reference gene selection 

for quantitative real-time RT-PCR normalization in the half-smooth tongue sole (Cynoglossus 

semilaevis) at different developmental stages, in various tissue types and on exposure to 

chemicals. PLoS ONE, 9(3), 1-10.

 

 

Liu, G. M., Cao, M. J., Huang, Y. Y., Cai, Q. F., Weng, W. Y., & Su, W. J. (2010).

Comparative study of in vitro digestibility of major allergen tropomyosin and other food proteins 

of Chinese mitten crab (Eriocheir sinensis). Journal of the Science of Food and Agriculture, 

90(10), 1614-1620.

 

Liu, G. M., Li, B., Yu, H. L., Cao, M. J., Cai, Q. F., Lin, J. W., et al. (2012). Induction of mud 

crab (Scylla paramamosain) tropomyosin and arginine kinase specific hypersensitivity in BALB/c 

mice. Journal of the Science of Food and Agriculture, 92(2), 232-238.

 

 

Liu, H., Chen, R., Zhang, Q., Peng, H., & Wang, K. (2011). Differential gene expression profile 

from haematopoietic tissue stem cells of red claw crayfish, Cherax

quadricarinatus, in response to WSSV infection. Developmental and Comparative

Immunology, 35, 716-724.

 

Liu, M., Liu, G., Yang, Y., Mei, X., Yang, H., Li, Y., et al. (2018). Thermal processing influences 

the digestibility and immunoreactivity of muscle proteins of Scylla paramamosain. LWT - Food 

Science and Technology, 98, 559-567.

 

 

Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using Real-Time 

Quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 25, 402-408.

 

 

Lopata, A. L., Kleine-Tebbe, J., & Kamath, S. D. (2016). Allergens and molecular diagnostics of 

shellfish allergy. Allergo Journal, 25(7), 24-32.

 

 

Lopez-Matas, M. A., Iraola, V., Moya, R., Vailes, L. D., Pomes, A., Boquete, M., et al. (2015). 

Cloning and characterization of tropomyosin from the mite Chortoglyphus arcuatus. Molecular 

Immunology, 68(2), 634-640.

 

 

Luo, W., Hu, H., Tang, W., Zou, X., Huang, H., Huang, Z., et al. (2019). Allergen sensitization 

pattern of allergic adults and children in Southern China: a survey based on real life data. 

Allergy, Asthma and Clinical Immunology, 15(1), 1-9.

 

 

Ma, H., Ma, C., & Ma, L. (2012). Molecular identification of genus Scylla (Decapoda: Portunidae)  

based  on DNA  barcoding and polymerase  chain  reaction. Biochemical Systematics and Ecology, 41, 

41-47.

 

 

Ma, H., Ma, C., & Ma, L. (2012). High genetic diversity and low differentiation in mud crab (Scylla 

paramamosain) along the southeastern coast of China revealed by microsatellite markers. The Journal 

of Experimental Biology, 215(17), 3120-3125.

 

 

Ma, H., Ma, C., Ma, L., & Li, X. (2016). Microsatellite Markers in the mud crab (Scylla 

paramamosain) and their application in population genetics and marker-assited selection. In 

Microsatellite Markers (pp. 165-183).

 

 

Mak, T. W., & Saunders, E. (2006). Allergy and hypersensitivity. In The Immune Response (pp. 

923–962).

 

 

Mandal, A., Varkey, M., & Pindaniyil, S. (2014). Molecular markers reveal only two mud crab species 

of Genus Scylla (Brachyura: Portunidae) in Indian Coastal Waters. Biochemical Genetics, 1(52), 

338-354.

 

 

Mao, H., Cao, M., Maleki, S. J., Cai, Q., Su, W., Yang, Y., et al. (2013). Structural 

characterization and IgE epitope analysis of arginine kinase from Scylla

paramamosain. Molecular Immunology, 56(4), 463-470.

 

Mao, S., Wu, L., & Shi, W. (2020). Prevalence and distribution patterns of allergens among children 

with asthma and asthma-like symptoms in Shanghai, China. Respiratory Research, 21(57), 4-11.

 

Matricardi, P. M., Kleine-Tebbe, J., Hoffmann, H. J., Valenta, R., Hilger, C., Hofmaier, S., et al. 

(2016). EAACI Molecular Allergology User’s Guide. Pediatric Allergy and Immunology, 27, 1-250.

 

 

Matsuo, H., Yokooji, T., & Taogoshi, T. (2015). Common food allergens and their IgE- binding 

epitopes. Allergology International, 64(4), 332-343.

 

 

Mattos, C. P. D., Dantas, F. J. S., Caldeira-de-Araujo, A., & Moraes, M. O. J. (2004). Agarose gel 

electrophoresis system in the classroom. Biochemistry and Biology Education, 32(4), 254-257.

 

Mayze, J. (2013). Alive and kicking: Best practice handling of live mud crabs for retailers 

(Retrieved from https://www.daf.qld.gov.au/).

 

Mehennaoui, K., Legay, S., Serchi, T., Guerold, F., Giamberini, L., Gutleb, A. C., et al. (2018). 

Identification of reference genes for RT-qPCR data normalization in Gammarus fossarum (Crustacea 

Amphipoda). Scientific Reports, 8, 1-8.

 

 

Melen, E., Pomes, A., Vailes, L. D., Arruda, L. K., & Chapman, M. D. (1999). Molecular cloning of 

per a 1 and definition of the cross-reactive Group 1 cockroach allergens. Journal of Allergy and 

Clinical Immunology, 103, 859-864.

 

 

Melo, B., Rezende, L., Machado, P., Gouveia, N., & Levy, R. (2018). Associations of ultra-processed 

food and drink products with asthma and wheezing among Brazilian adolescents. Pediatric Allergy and 

Immunology, 29(5), 504-511.

 

 

Milian, E., & Diaz, A. M. (2004). Allergy to house dust mites and asthma. Puerto Rico Health 

Sciences Journal, 23(1), 47-57.

 

 

Millier, L., & Green, P. (1991). OSP: A computer program for choosing PCR and DNA sequencing 

primers. Genome Research, 1(2), 124-128.

 

Motoyama, K., Suma, Y., Ishizaki, S., Naashima, Y., Lu, Y., Ushio, H., & Shiomi, K. (2008). 

Identification of tropomyosins as major allergens in Antarctic krill and mantis  shrimp  and  their 

 amino  acid  sequence  characteristics.  Marine Biotechnology, 10(6), 709-718.

 

Motoyama, K., Suma, Y., Ishizaki, S., Nagashima, Y., & Shiomi, K. (2007). Molecular

 

Kurochkina, N. (2010). Helix-helix interactions and their impact on protein motifs and assemblies. 

Journal of Theoretical Biology, 264(2), 585-592.

 

 

Kurowski, K., & Boxer, R. W. (2008). Food Allergies: detection and management.

American Family Physician, 77(12), 1678-1686.

cloning of tropomyosins identified as allergens in six species of crustaceans.

Journal of Agricultural and Food Chemistry, 55(3), 985-991.

 

 

Muhd-Farouk, H., Nurul Huda, A., Abol-Munafi, A. B., Mardhiyyah, M. P., Nurul Hasyima, I., Hidayah, 

M., et al. (2019). Development of ovarian maturations in orange mud crab, Scylla olivacea (Herbst, 

1796) through induction of eyestalk ablation and methyl farnesoate. Arab Journal of Basic and 

Applied Sciences, 26(1), 171-181.

 

Munera, M., Contreras, N., Sanchez, A., Sanchez, J., & Emiliani, Y. (2019). In silico analysis of a 

major allergen from Rattus norvegicus, Rat n 1, and cross-reactivity with domestic pets. 

F1000Research, 8, 1-18.

 

 

Mustafa, N., Rosmilah, M., Zailatul Hani, M. Y., Faizal, B., Noormalin, A., & Hasan, A. (2018). 

Comparison of protein profiles and allergenicity of different body parts and genders of Scylla 

paramamosain. International Journal of Science, Environment and Technology, 7(5), 1759-1765.

 

Nakano, S., Yoshinuma, T., & Yamada, T. (2008). Reactivity of shrimp allergy-related IgE antibodies 

to krill tropomyosin. International Archives of Allergy and Immunology, 145(3), 175-181.

 

 

Naz, S., Desclozeaux, M., Mounsey, K. E., Chaudhry, F. R., & Walton, S. F. (2017). Characterization 

 of  Sarcoptes  scabiei  tropomyosin  and  paramyosin: immunoreactive allergens in scabies. 

American Journal of Tropical Medicine and Hygiene, 97(3), 851-860.

 

Nehete, J. Y., Bhambar, R. S., Narkhede, M. R., & Gawali, S. R. (2013). Natural proteins: Sources, 

isolation, characterization and applications. Pharmacognosy Reviews, 7(14), 107-117.

 

 

Nugraha, R., Kamath, S. D., Johnston, E., Karnaneedi, S., Ruethers, T., & Lopata, A.

L. (2019). Conservation analysis of B-cell allergen epitopes to predict clinical cross-reactivity 

between shellfish and inhalant invertebrate allergens. Frontiers in Immunology, 10, 1-13.

 

Nurul Ain, M. S., Noor Amalia, Rodrigues, K., Ransangan, J., & Seok-Kian, A. Y. (2016). Genetic 

diversity of mud crabs, Scylla tranquebarica in Sabah, Malaysia based on Cytochrome C Oxidase (COI) 

gene sequence. Songklanakarin Journal of Science and Technology, 38(4), 365-372.

 

Nurul Ain, M. S., Noor Amalia, S. K., Muhammad Ali, S. H., Julian, R., & Seok-Kian, A.

Y. (2019). Species diversity and distribution of mud crab in Marudu Bay mangrove forest reserve, 

Sabah, Malaysia. Borneo Journal of Marine Science and

Aquaculture, 3(1), 18-24.

 

Nurul Izzah, A. R., Rosmilah, M., & Zailatul Hani, M. Y. (2015). Identification of major and minor 

allergens of mud crab (Scylla Serrata). Medicine and Health, 10(2), 90- 97.

 

Ogawa, C. Y., Hamasaki, K., Dan, S., Obata, Y., & Kitada, S. (2012). Species composition, 

reproduction, and body size of mud crabs, Scylla spp., caught in Urado Bay, Japan. Journal of 

Crustacean Biology, 32(5), 762-768.

 

 

Okayama, H. (2012). Functional cDNA expression cloning: pushing it to the limit. Proceedings of the 

Japan Academy Series B: Physical and Biological Sciences, 88(3), 102-119.

 

 

Overton, J. L., & Macintosh, D. J. (2002). Estimated size at sexual maturity for female mud crabs 

(Genus Scylla) from two sympatric species within Ban Don Bay, Thailand. Journal of Crustacean 

Biology, 22(4), 790-797.

 

Owczarzy, R., Tataurov, A. V., Wu, Y., Manthey, J. A., McQuisten, K. A., Almabrazi,

H. G., et al. (2008). IDT SciTools: a suite for analysis and design of nucleic acid oligomers. 

Nucleic Acids Research, 36, 163-169.

 

Padate, V. P., Rivonker, C. U., & Ail, A. C. (2013). A new record of Scylla olivacea (Decapoda, 

Bachyura, Prtunidae) from Goa, central west coast of India- A comparative diagnosis. Indian Journal 

of Marine Sciences, 42(1), 82-89.

 

Park, H., Lee, J., Park, K. H., Ann, H. W., Jin, M., Choi, S., et al. (2014). A nationwide survey 

of inhalant allergens sensitization and levels of indoor major allergens in Korea. Allergy, Asthma 

and Immunology Research, 6(3), 222-227.

 

 

Parvin, I., Islam, K. M. I., Hoq, M. E., & Alam, M. S. (2018). Morphometric identification of mud 

crabs of the Genus Scylla available in Bangladesh waters. Bangladesh Journal of Fisheries, 30(2), 

19-206.

 

 

Patel, B. Y., & Volcheck, G. W. (2015). Food allergy: Common causes, diagnosis, and treatment. Mayo 

Clinic Proceedings, 90(10), 1411-1419.

 

 

Pekar, J., Ret, D., & Untersmayr, E. (2018). Stability of allergens. Molecular Immunology, 100, 

14-20.

 

 

Pfaffl, M. W., Tichopad, A., Prgomet, C., & Neuvians, T. P. (2004). Determination of stable 

housekeeping genes, differentially regulated target genes and sample integrity :  

BestKeeper-Excel-based  tool  using  pair-wise  correlations.

Biotechnology Letters, 26, 509-515.

 

Phinyo, M., Visudtiphole, V., Roytrakul, S., Phaonakrop, N., Jarayabhand, P., & Klinbunga, S. 

(2013). General and comparative endocrinology characterization and expression of cell division 

cycle 2 (Cdc2) mRNA and protein during ovarian development of the giant tiger shrimp Penaeus 

monodon. General and Comparative Endocrinology, 193, 103-111.

 

Piboonpocanun, S., Malainual, N., Jirapongsananuruk, O., Vichyanond, P., & Thomas,

W. R. (2006). Genetic polymorphisms of major house dust mite allergens. Clinical and Experimental 

Allergy, 36(4), 510-516.

 

 

Pittenger, M. F., Kazzaz, J. A., & Helfman, D. M. (1994). Functional properties of non- muscle 

tropomyosin isoforms. Current Opinion in Cell Biology, 6(1), 96-104.

 

 

Pomes, A., Davies, J. M., Gadermaier, G., Hilger, C., Holzhauser, T., Lidholm, J., et al. (2018). 

WHO/IUIS allergen nomenclature: providing a common language. Molecular Immunology, 100, 3-13.

 

 

Poms, R. E., & Anklam, E. (2005). Tracking and tracing for allergen-free food production chains. 

Frontis, 10, 77-83.

 

Prasanthi, C., & Ramesh, K. B. (2019). Comparative evaluation of Scylla species from two fishery 

conservation areas of east coast of Andhra Pradesh, India. International Journal of Bio-Pharma 

Research, 8(5), 2593-2601.

 

 

Quinitio, E. T., Pedro, J. De, & Parado-estepa, F. D. (2007). Ovarian maturation stages of the mud 

crab Scylla serrata. Aquaculture Research, 38, 1434-1441.

 

 

Ramos, J. D. A., Cheong, N., Lee, B. W., & Chua, K. Y. (2001). cDNA cloning and expression of Blo t 

11, the Blomia tropicalis allergen homologous to paramyosin. International Archives of Allergy and 

Immunology, 126(4), 286-293.

 

 

Reese, G., Ayuso, R., & Lehrer, S. B. (1999). Tropomyosin: An invertebrate pan- allergen. 

International Achieves of Allergy and Immunology, 119, 247-258.

 

 

Ridgeway, J. A., & Timm, A. E. (2015). Reference gene selection for quantitative real- time PCR 

normalization in larvae of three species of Grapholitini (Lepidoptera: Tortricidae). PLoS ONE, 

10(6), 1-15.

 

 

Rolland, J. M., Varese, N. P., Abramovitch, J. B., Anania, J., Nugraha, R., Kamath, S., et al. 

(2018). Effect of heat processing on IgE reactivity and cross-reactivity of tropomyosin and other 

allergens of Asia-pacific mollusc species: identification of novel Sydney rock oyster tropomyosin 

Sac g 1. Molecular Nutrition and Food

Research, 62(14), 1-12.

 

Rombel, I. T., Sykes, K. F., Rayner, S., & Johnston, S. A. (2002). ORF-FINDER: a

vector for high-throughput gene identification. Gene, 282, 33-41.

 

 

Rosmilah, M., Nurul Izzah, A. R., Zailatul Hani, M. Y., Noormalin, A., Mohd Faizal, B., & Shahnaz, 

M. (2015). Characterization of major allergens of local mud crab (Scylla serrata). Scientific 

REsearch Journal, 12(1), 1-10.

 

 

Rosmilah, M., Shahnaz, M., Zailatul Hani, M. Y., & Noormalin, A. (2012). Identification of the 

major allergens of Charybdis feriatus (red crab) and its cross-reactivity with Portunus pelagicus 

(blue crab). Asian Pacific Journal of Allergy and Immunology, 30(4), 285-293.

 

Rosmilah, M., Shahnaz, M., Zailatul Hani, M. Y., Noormalin, A., & Normilah, I. (2012). 

Identification of tropomyosin and arginine kinase as major allergens of Portunus pelagicus (blue 

swimming crab). Tropical Biomedicine, 29(3), 467-478.

 

 

Rouf, M. A., Shahriar, S. I., Sarower, M. G., & Ahsan, M. A. (2016). Taxonomic clarification of mud 

crab species of Genus Scylla (Brachyura: Portunidae) available in the coastal regions of 

Bangladesh. Asian Fisheries Science, 29, 124- 136.

 

 

Rougeon, F., Kourilsky, P., & Mach, B. (1975). Insertion of a rabbit β-globin gene sequence into an 

E. coli plasmid. Nucleic Acids Research, 2(12), 2365-2378.

 

Ruethers, T., Taki, A. C., Johnston, E. B., Nugraha, R., Le, T. T. K., Kalic, T., et al. (2018). 

Seafood allergy: a comprehensive review of fish and shellfish allergens. Molecular Immunology, 100, 

28-57.

 

 

Ruhana, H., & Durrah Syazwani, M. (2019). Morphology and genetic diversity of mud crabs 

(Portunidae: Scylla) from Teluk Sulaman, Sabah, Malaysian Borneo. ASM Science Journal, 12, 1-9.

 

 

Saetang, J., Tipmanee, V., & Benjakul, S. (2022). In silico prediction of cross-reactive epitopes 

of tropomyosin. Molecules, 27(2667), 1-15.

 

 

Sampson, H. A. (2003). Anaphylaxis and emergency treatment. Pediatrics, 111, 1601- 1608.

 

 

Sampson, H. A., Gerth V. W. R., Bindslev-Jensen, C., Sicherer, S., Teuber, S. S., Burks, A. W., et 

al. (2012). Standardizing double-blind, placebo-controlled oral food challenges: American Academy 

of Allergy, Asthma & Immunology-European Academy of Allergy and Clinical Immunology PRACTALL 

consensus report.

Journal of Allergy and Clinical Immunology, 130(6), 1260-1274.

 

Santos, A. B. R., Chapman, M. D., Aalberse, R. C., Vailes, D., Virginia, P., Ferriani, L., et al. 

(1999). Cockroach allergens and asthma in Brazil: Identification of tropomyosin as a major allergen 

with potential cross-reactivity with mite and shrimp allergens. Journal of Allergy and Clinical 

Immunology, 104(2), 329-337.

 

Shafique, R. H., Inam, M., Ismail, M., & Chaudhary, F. R. (2013). Group 10 allergens (tropomyosins) 

from house-dust mites may cause covariation of sensitization to allergens from other invertebrates. 

Allergy & Rhinology, 3(2), 74-90.

 

 

Sharma, G. M., Khuda, S. E., Parker, C. H., Eischeid, A. C., & Pereira, M. (2017). Detection of 

allergen markers in food: analytical methods. Food and Drug Administration Paper, 6, 65-121.

 

 

Sheikh, S. N., & Lazarus, P. (1997). Re-usable DNA template for the polymerase chain reaction 

(PCR). Nucleic Acids Research, 25(17), 3537-3542.

 

Shekhar, M. S., Kiruthika, J., & Ponniah, A. G. (2013). Identification and expression analysis of 

differentially expressed genes from shrimp (Penaeus monodon) in response to low salinity stress. 

Fish and Shellfish Immunology, 35(6), 1957-1968.

 

 

Shen, Y., Cao, M. J., Cai, Q. F., Su, W. J., Yu, H. L., Ruan, W. W., et al. (2011).

Purification, cloning, expression and immunological analysis of Scylla serrata arginine kinase, the 

crab allergen. Journal of the Science of Food and Agriculture, 91(7), 1326-1335.

 

 

Sherson, D., Hansen, I., & Sigsgaard, T. (1989). Occupationally related respiratory symptoms in 

trout‑processing workers. Allergy, 44(5), 336-341.

 

Shiao, Y. (2003). A new reverse transcription-polymerase chain reaction method for accurate 

quantification. BMC Biotechnology, 3, 1-12.

 

 

Simossis, V. A., & Heringa, J. (2005). PRALINE: A multiple sequence alignment toolbox that 

integrates homology-extended and secondary structure information. Nucleic Acids Research, 33, 

289-294.

 

 

Singh, M. B., & Bhalla, P. L. (2003). Hypoallergenic derivatives of major grass pollen allergens 

for allergy vaccination. Immunology and Cell Iology, 81, 86-91.

 

 

Smoldovskaya, O., Feyzkhanova, G., Arefieva, A., Voloshin, S., Ivashkina, O., Reznikov, Y., et al. 

(2016). Allergen extracts and recombinant proteins: comparison of efficiency of in vitro allergy 

diagnostics using multiplex assay on a

biological microchip. Allergy, Asthma & Clinical Immunology, 12(9), 1-5.

 

Soegiarto, G., Mai Shihah, A., Damayanti, L. A., Suseno, A., & Effendi, C. (2019). The prevalence 

of allergic diseases in school children of metropolitan city in Indonesia shows a similar pattern 

to that of developed countries. Asia Pacific Allergy, 9(2), 1-10.

 

Song, J., Li, L., Liu, Z., Li, Q., & Ran, P. (2009). Sequence analysis and expression of a cDNA 

clone encoding tropomyosin in Sinonovacula constricta. Molecular Biology Reports, 36(2), 315-321.

 

 

Sookrung, N., Indrawattana, N., Tungtrongchitr, A., Bunnag, C., Tantilipikorn, P., Kwangsri, S., et 

al. (2009). Allergenicity of native/ recombinant tropomyosin, per a 7, of American Cockroach (CR), 

Periplaneta americana, among CR Allergic Thais. Asian Pacific Journal of Allergy and Immunology, 

27(1), 9-17.

 

 

Stanley, P. (2011). Golgi glycosylation. Cold Sring Harbor Perspective Biology, 3, 1- 13.

 

 

Stellwagen, N. C., & Stellwagen, E. (2009). Effect of the matrix on DNA electrophoretic mobility. 

Journal of Chromatography A, 1216(10), 1917-1929.

 

Steven, A. L., & Huszalina, H. (2022). In silico analysis of maturation and flocculating protein 

(rbcl) from d unaliella salina in wastewater treatment. Proceedings of Science and Mathematics, 

13(2007), 185-195.

 

Su, X., Lu, L., Li, Y., Zhen, C., Hu, G., Jiang, K., et al. (2020). Reference gene selection for 

quantitative realtime PCR (qRT-PCR) expression analysis in Galium aparine

L. PLoS ONE, 15(2), 1-12.

 

 

Sun, B. Q., Zheng, P. Y., Zhang, X. W., Huang, H. M., Chen, D. H., & Zeng, G. Q. (2014). Prevalence 

of allergen sensitization among patients with allergic diseases in Guangzhou, Southern China: a 

four-year observational study. Multidisciplinary Respiratory Medicine, 9(2), 1-9.

 

 

Sun, X., Zhao, J., Wang, Q., Shi, G., Yang, J., & Ming, L. (2019). Prevalence of allergen 

sensitization among 15,534 patients with suspected allergic diseases in Henan Province, China. 

Asian Pacific Journal of Allergy and Immunology, 37(2), 57-64.

 

 

Synnergren, J., Giesler, T. L., Adak, S., Tandon, R., Noaksson, K., Lindahl, A., et al. (2007).  

Differentiating  human  embryonic  stem  cells  express  a  unique housekeeping gene signature. 

Stem Cells, 25, 473-480.

 

 

Takeharu, K. (2001). Brief assessment of stock of mud crabs Scylla spp. in Matang Mangrove forest, 

Malaysia and proposal for resources management. Japan

Agricultural Research Quarterly, 35(2), 5-8.

 

Tajsharghi, H. (2008). Thick and thin filament gene mutations in striated muscle diseases. 

International Journal of Molecular Sciences, 9, 1259-1275.

 

Takai, T., & Izuhara, K. (2015). Allergology international allergens in modern society: updated 

catalogs and future prospects. Allergology International, 64(4), 293-294.

 

Teng, F., Yu, L., Sun, J., Wang, N., & Cui, Y. (2018). Homology modeling and prediction of B-cell 

and T-cell epitopes of the house dust mite allergen der f 20. Molecular Medicine Reports, 17(1), 

1807-1812.

 

 

Thomas, W. R., Smith, W., Hales, B. J., Mills, K. L., & O’Brien, R. M. (2002). Characterization and 

immunobiology of house dust mite allergens. International Archives of Allergy and Immunology, 

129(1), 1-18.

 

 

Tokmakov, A. A., Kurotani, A., & Sato, K. (2021). Protein pI and intracellular localization. 

Frontier in Molecular Biosciences, 8, 1-6.

 

 

Tong, W. S., Yuen, A. W. T., Wai, C. Y. Y., Leung, N. Y. H., Chu, K. H., & Leung, P. S.

C. (2018). Diagnosis of fish and shellfish allergies. Journal of Asthma and Allergy, 11, 247-260.

 

 

Toth, E., Huszaar, K., Bencsura, P., Kulcsar, P. I., Vodicska, B., Nyeste, A., et al. (2014). 

Restriction enzyme body doubles and PCR cloning: on the general use of Type IIS restriction enzymes 

for cloning. PLoS ONE, 9(3), 1-12.

 

 

Traidl-Hoffmann, C., Jakob, T., & Behrendt, H. (2009). Determinants of allergenicity.

Journal of Allergy and Clinical Immunology, 123(3), 558-566.

 

 

Tweedie, J. W., & Stowell, K. M. (2005). Quantification of DNA by agarose gel electrophoresis and 

analysis of the topoisomers of plasmid and M13 DNA following treatment with a restriction 

endonuclease or DNA topoisomerase I. Biochemistry and Molecular Biology Education, 33(1), 28-33.

 

Udvardi, M. K., Czechowski, T., & Scheible, W. R. (2008). Eleven golden rules of quantitative 

RT-PCR. Plant Cell, 20(7), 1736-1737.

 

 

Untergasser, A., Nijveen, H., Rao, X., Bisseling, T., Geurts, R., & Leunissen, J. A. M. (2007). 

Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Research, 35, 71-74.

 

 

Valenta, R., & Niederberger, V. (2007). Recombinant allergens for immunotherapy.

Journal of Allergy and Clinical Immunology, 119(4), 826-830.

 

Vandesompele, J., Preter, K. D., Pattyn, F., Poppe, B., Roy, N. V., Paepe, A. D., et al. (2002). 

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple 

internal control genes. Genome Biology, 3(7), 1- 12.

 

Vay, L. L., Ut, V. N., & Jones, D. A. (2001). Seasonal abundance and recruitment in an estuarine 

population of mud crabs, Scylla paramamosain, in the Mekong Delta, Vietnam. Hydrobiologia, 449, 

231-240.

 

 

Verhoeckx, K. C. M., Vissers, Y. M., Baumert, J. L., Faludi, R., Feys, M., Flanagan, S., et al. 

(2015). Food processing and allergenicity. Food and Chemical Toxicology, 80, 223-240.

 

 

Vincze, T., Posfai, J., & Roberts, R. J. (2003). NEBcutter: A program to cleave DNA with 

restriction enzymes. Nucleic Acids Research, 31(13), 3688-3691.

 

Viswanathan, C., & Raffi, S. M. (2015). The natural diet of the mud crab Scylla olivacea (Herbst, 

1896) in Pichavaram mangroves, India. Saudi Journal of Biological Sciences, 22(6), 698-705.

 

 

Wachholz, P. A., Dearman, R. J., & Kimber, I. (2005). Detection of allergen-specific IgE antibody 

responses. Journal of Immunotoxicology, 1(3–4), 189-199.

 

 

Waiho, K., Fazhan, H., Baylon, J. C., Norfaizza, W. I. W., & Ikhwanuddin, M. (2016). Use of abdomen 

looseness as an indicator of sexual maturity in male mud crab Scylla spp. Journal of Shellfish 

Research, 35(4), 1027-1035.

 

Waiho, K., Mustaqim, M., Fazhan, H., Wan Norfaizza, W. I., Megat, F. H., & Ikhwanuddin, M. (2015). 

Mating behaviour of the orange mud crab, Scylla olivacea: The effect of sex ratio and stocking 

density on mating success. Aquaculture Reports, 2, 50-57.

 

 

Wan, K. S., Yang, W., & Wu, W. F. (2010). A survey of serum specific-IgE to common allergens in 

primary school children of Taipei city. Asian Pacific Journal of Allergy and Immunology, 28(1), 

1-6.

 

 

Wan Majdiah, W. M., Nurul Khaiza, Y., Suzina, S. A. H., Che Maraina, C. H., & Noor Suryani, M. A. 

(2016). Food allergen Sensitization among Malaysian rhinitis patients. International Medical 

Journal, 23(3), 252-255.

 

 

Wang, L., Zhang, X., Zhang, J., Fan, W., Lu, M., & Hu, J. (2018). Proteomic analysis and 

identification of possible allergenic proteins in mature pollen of Populus

tomentosa. International Journal of Molecular Sciences, 19(1), 250-265.

 

Wang, X., Li, L., Xu, F., & Zhang, G. (2011). Tropomyosin is a nice marker gene for phylogenetic 

analysis of molluscs. Molecular Biology Reports, 38(7), 4589-4593.

 

Wheatley, L. M., & Togias, A. (2017). Allergic rhinitis. The New England Journal of Medicine, 

372(5), 351-372.

 

Wilkins, M. R., Gasteiger, E., Bairoch, A., Sanchez, J. C., Williams, K. L., Appel, R. D., & 

Hochstrasser, D. F. (1999). Protein identification and analysis tools in the ExPASy server. In 

Methods in Molecular Biology, 112, 531-552.

 

 

Woo, C. K., & Bahna, S. L. (2011). Not all shellfish “allergy” is allergy! Clinical and 

Translational Allergy, 1(3), 1-12.

 

 

Wu, C. C., Lee, C. H., Tyan, Y. C., Huang, E. S., Yu, W. T., & Yu, H. S. (2019).

Identification of pyruvate kinase 2 as a possible crab allergen and analysis of allergenic proteins 

in crabs consumed in Taiwan. Food Chemistry, 289, 413-418.

 

 

Wu, T. C., Tsai, T. C., Huang, C. F., Chang, F. Y., Lin, C. C., Huang, I. F., et al. (2012). 

Prevalence of food allergy in Taiwan: A questionnaire-based survey. Internal Medicine Journal, 

42(12), 1310-1315.

 

 

Xia, F., Li, M., Liu, Q., Liu, M., Yang, Y., Cao, M., et al. (2019). Crystal structure analysis and 

conformational epitope mutation of triosephosphate isomerase, a mud crab allergen. Journal of 

Agricultural and Food Chemistry, 67(46), 12918- 12926.

 

Xie, F., Xiao, P., Chen, D., Xu, L., & Zhang, B. (2012). miRDeepFinder: a miRNA analysis tool for 

deep sequencing of plant small RNAs. Plant Molecular Biology, 80(1), 75-84.

 

 

Xu, J., Cao, J., Cao, D., Zhao, T., Huang, X., Zhang, P., et al. (2013). Flanking sequence 

determination and event-specific detection of genetically modified wheat B73-6-1. Acta Biochimica 

et Biophysica Sinica, 45(5), 416-421.

 

 

Xu, L., Zhang, H., Zhang, X., Lin, H., Guo, Y., Yu, C., et al. (2020). Natural shrimp (Litopenaeus 

vannamei) tropomyosin shows higher allergic properties than recombinant ones as compared through 

SWATH-MS-based proteomics and immunological response. Journal of Agricultural and Food Chemistry, 

68(41), 11553-11567.

 

Yang, H., Chen, H., Jin, M., Xie, H., He, S., & Wei, J. (2016). Molecular cloning, expression, IgE 

binding activities and in silico epitope prediction of Per a 9 allergens of the American cockroach. 

International Journal of Molecular Medicine,

38(6), 1795-1805.

 

Yang, Y., Cao, M., Alcocer, M., Liu, Q., Fei, D., Mao, H., et al. (2015). Mapping and 

characterization of antigenic epitopes of arginine kinase of Scylla paramamosain. Molecular 

Immunology, 65(2), 310-320.

 

Yang, Y., Hu, M. J., Jin, T. C., Zhang, Y. X., Liu, G. Y., Li, Y. B., et al. (2019). A

comprehensive analysis of the allergenicity and IgE epitopes of myosinogen allergens in Scylla 

paramamosain. Clinical and Experimental Allergy, 49(1), 108- 119.

 

 

Yang, Y., Liu, G., Yang, H., Hu, M., Cao, M., Su, W., et al. (2019). Crystal structure 

determination of Scylla paramamosain arginine kinase, an allergen that may cause cross-reactivity 

among invertebrates. Food Chemistry, 271, 597-605.

 

 

Yang, Z., Zheng, W., Yung, E., Zhong, N., Wong, G. W. K., & Li, J. (2015). Frequency of food group 

consumption and risk of allergic disease and sensitization in school children in urban and rural 

China. Clinical and Experimental Allergy, 45(12), 1823- 1832.

 

Yu, H., Cao, M., Cai, Q., Weng, W., Su, W., & Liu, G. (2011). Effects of different processing  

methods  on  digestibility  of  Scylla  paramamosain  allergen (tropomyosin). Food and Chemical 

Toxicology, 49(4), 791-798.

 

 

Yu, H., Ruan, W., Cao, M., Cai, Q., Shen, H., & Liu, G. (2013). Identification of physicochemical 

properties of Scylla paramamosain allergen, arginine kinase. Journal of the Science of Food and 

Agriculture, 93(2), 245-253.

 

Yu, K., Gong, J., Huang, C., Huang, H., & Ye, H. (2015). Characterization of CCTα and evaluating 

its expression in the mud crab Scylla paramamosain when challenged by low temperatures alone and in 

combination with high and low salinity. Cell Stress and Chaperones, 20(5), 853-864.

 

Zaffagnini, M., Fermani, S., Costa, A., Lemaire, S. D., & Trost, P. (2013). Plant cytoplasmic 

GAPDH: redox post-translational modifications and moonlighting properties. Frontiers in Plant 

Science, 4(450), 1-18.

 

 

Zailatul Hani, M. Y., Leecyous, B., & Amelia Suhana, Z. (2020). Isolation and cloning of 

tropomyosin and arginine kinase from tiger prawn Penaeus monodon and blue swimming crab Portunus 

trituberculatus. Journal of Science and Mathematics Letters, 8(2), 2019-2021.

 

Zailatul Hani, M. Y., Rosmilah, M., Faizal, B., Noormalin, A., & Shahnaz, M. (2015). Tropomyosin 

and actin identified as major allergens of the carpet clam (Paphia textile) and the effect of 

cooking on their allergenicity. BioMed Research

International, 2015, 13-18.

 

Zailatul Hani, M. Y., Rosmilah, M., Noormalin, A., Faizal, B., Masita, A., & Shahnaz,

M. (2010). Identification of IgE-binding proteins of raw and cooked extracts of Loligo edulis 

(white squid). Southeast Asian Journal of Tropical Medicine and Public Health, 41(3), 653-659.

 

Zhang, J., & Tao, A. (2015). Antigenicity, immunogenicity, allergenicity. Allergy Bioinformatics, 

Translational Bioinformatics (pp. 175-c186).

 

 

Zhang, Y., Matsuo, H., & Morita, E. (2006). Cross-reactivity among shrimp, crab and scallops in a 

patient with a seafood allergy. Journal of Dermatology, 33(3), 174- 177.

 

 

Zhang, Z., Li, X., Xiao, H., Nowak-Wegrzyn., A., & Z., P. (2020). IgE-binding epitope mapping of 

tropomyosin allergen (Exo m 1) from Exopalaemon modestus, the freshwater Siberian prawn. Food 

Chemistry, 309 (1-9).

 

 

Zhou, S., Tao, Z., Shen, C., Qian, D., Wang, C., & Zhou, Q. (2018). β-actin gene expression is 

variable among individuals and not suitable for normalizing mRNA levels in Portunus 

trituberculatus. Fish and Shellfish Immunology, 81, 338-342.

 

 

Zhu, L., She, T., Zhang, Y., Li, S., Xu, Z., Yan, J., et al. (2016). Identification and 

characterization of ovary development-related protein EJO1 (Eri s 2) from the ovary of Eriocheir 

sinensis as a new food allergen. Molecular Nutrition & Food Research, 60(10), 2275-2287.

 

 

Zuberbier, T., Edenharter, G., Worm, M., Ehlers, I., Reimann, S., Hantke, T., et al. (2004). 

Prevalence of adverse reactions to food in Germany- a population study. Allergy: European Journal 

of Allergy and Clinical Immunology, 59(3), 338-345.

 

 

Zukiewicz-Sobczak, W. A., Wroblewska, P., Adamczuk, P., & Kopczynski, P. (2013). Causes, symptoms 

and prevention of food allergy. Postepy Dermatologii

Alergologii, 30(2), 113-116.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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