UPSI Digital Repository (UDRep)
|
UPSI Digital Repository (UDRep)
|
|
|
|
||||||||||||||||||||||||||
| Abstract : Perpustakaan Tuanku Bainun |
| Brassica oleracea var. italica is an important vegetable crops mostly grown in Cameron Highland, Malaysia. These vegetables are highly vulnerable to high temperature and grow best in cool climate. The purpose of this research was to examine in vitro growth response of B. oleracea var. italica through tissue culture system. Complete plant regeneration was successfully achieved when root, stem and leaf aseptic explant was cultured on MS medium supplemented with various combination of plant growth regulators. The experimental design used in this study was Completely Randomized Design (CRD). MS medium supplemented with 1.0 mg/L IBA + 0.5 mg/L BAP was found to be the optimum medium for shoot regeneration with 5.367±0.768 shoots per explant. Meanwhile 2.0 mg/L NAA was the optimum medium for leaf and root explant for root induction producing 7.567±0.498 and 8.767±0.707 roots per explant. Optimum callus induction was obtained when leaf explants were cultured on MS medium supplemented with 1.0 mg/L NAA ± 1.5 mg/L BAP. Friable cream callus were produced. Meanwhile synthetic seeds of B. oleracea var. italica were produced when micro shoots were encapsulated with 4.0% sodium alginate solution added with 1.5 mg/L BAP + 1.0 mg/L NAA. Synthetic seeds germination rate was 4.532±0.860 shoots per explant. Finally, acclimatization of B. oleracea var. italica was accomplished when plantlets were transferred to the combination of black and red soil (2:1) with 86.67% survival rate. In conclusion, this research showed that in vitro propagation of B. oleracea var. italica through tissue culture was successfully achieved. This research has provided positive implication in agriculture field which can be widely used to produce clones of a plant especially in Malaysia and also worldwide. |
| This material may be protected under Copyright Act which governs the making of photocopies or reproductions of copyrighted materials. You may use the digitized material for private study, scholarship, or research. |