UPSI Digital Repository (UDRep)
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UPSI Digital Repository (UDRep)
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| Abstract : Perpustakaan Tuanku Bainun |
| This study aimed to determine the effects of different storage conditions and chemical treatments
on protein profile and allergenicity of local edible sea snail, Cerithidea obtusa. Several snail
extracts from untreated, stored snails at different times and temperatures (4 °C, 25 °C and -20 °C)
and chemical-treated snails (salting and vinegar) were extracted and resolved by sodium
dodecyl sulfate-polyacrylamide gel electrophoresis (SOS-PAGE) to determine their protein
profiles. Allergenic properties of all snail extracts were then determined by immunoblotting using
sera from snail allergic patients. In SOS-PAGE, the untreated extract of snail resulted in at
least 26 protein bands, between molecular weights of 8 to >250 kDa. In contrast, fewer protein
bands (in range of 5-25) were seen in almost all stored snail extracts and chemical treated
extracts, except for the frozen snails which could retain their protein profiling as the untreated
snail. Immunoblotting of the untreated snail extract identified numerous IgE-binding proteins at
various molecular weights between 25 to 245 kDa. Four major allergens at 35, 48, I 00, and 135 kDa
were detected. Immunoblotting revealed that the untreated and all the frozen snail extracts had
more allergenic proteins than the other stored snail extracts and chemical-treated extracts. The
vinegar-treated extract was demonstrated to have the least allergenic proteins. As a conclusion, C.
obtusa had four major allergens and all extracts of untreated, stored and chemical-treated snails
played a crucial role in snail allergy. These results would directly contribute to advancements in
diagnosis,management and therapeutic methods of snail-allergic patients worldwide.
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