UPSI Digital Repository (UDRep)
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UPSI Digital Repository (UDRep)
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| Abstract : Perpustakaan Tuanku Bainun |
| Horseshoe crab populations are said to be declining worldwide. However, there is still no published
report on the status of horseshoe crabs in Malaysia. In this study, microsatellite markers were
developed using 5'anchored ISSR-PCR enrichment procedure to diagnose population genetic structure
of the mangrove horseshoe crab, Carcinoscorpius rotundicauda. A total of 134 microsatellite regions
have been succesfully isolated using this technique and 36 novel microsatellite markers were
developed. Out of these, eleven informative microsatellite loci were identified in an analysis of
127 samples representing five C. rolundicauda populations namely Kuala Juru, Kg. Pennatang, Kg.
Chuah, Kg. Sekokoh and Kg. Sg. Pulai from Peninsular Malaysia. The mean expected (He) and observed
heterozygosity (Ho) values obtained ranged between 0.299-0.42 l and 0.148-0.197 respectively.
Significant departures from Hardy-Weinberg equilibrium were detected m C. rotundicauda populations
at Kg. Permatang, Kg. Chuah and Kg. Sg. Pulai. These populations also show moderate genetic
differentiation with overall FsT values of 0.108. Pairwise genetic distance values analysed between the
five populations studied were close to zero which indicate high genetic similarity between populations.
Five polymorphic inter-simple-sequence-repeat (ISSR) markers tested also generated comparable results. Thus, samples
in each population are presumed to be genetically similar although the populations studied were
geographically distant.
Due to the unknown migratory ability of C. rotundicauda species, existing habitats must be
conserved for the survival of this species especially in Peninsular Malaysia. Findings from this
study provide information on the genetic diversity of C. rotundicauda in Peninsular Malaysia and
are hoped to assist in the development of conservation program for the mangrove horseshoe crabs in
Southeast Asia.
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