UPSI Digital Repository (UDRep)
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UPSI Digital Repository (UDRep)
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| Abstract : Perpustakaan Tuanku Bainun |
| Studies of Aquilaria crassna Pierre from the leaves and barks had been divided into four parts;
phytochemical screening, essential oils, chemical constituents and antimicrobial activities.
Samples were extracted using three solvents; hexane, dichloromethane and methanol. All
six extracts; three leaves and three barks had underwent phytochemical screening to detect
the presence of secondary metabolites. Fresh samples of leaves and barks went through
hydrodistillation process using Dean Stark apparatus to obtain essential oil. Isolation of
chemical compounds had been done using several chromatography techniques such as column
chromatography (CC), preparative thin layer chromatography (PTLC) and monitoring with thin
layer chromatography (TLC). Totally, seven pure compounds successively isolated. Four pure
compounds isolated from leaves; 5-hydroxy- 7,4• -dimethoxyflavone (29) and epifriedelanol
(30) from hexane extract while squalene (31) and 5-hydroxy-7,4• - dimethoxyflavone (29)
from dichloromethane extract. Further extraction on barks yield three pure compounds;
sec-nonacosyl ester (32), 5-hydroxy-7,4• - dimethoxytlavone (29) and bis (2-ethylhexyl)
phthalate (33). Finally, crude extracts had been screened for antimicrobial activities towards four
bacteria. Results show that Staphylococcus aureus was the most susceptible bacteria to all six
extracts. Whilst, the least susceptible strain was Pseudomonas aeruginosa.
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