Alteration of fatty acid compositions in a starch-deficient mutant Arabidopsis thaliana (adg1-1) during seed development

Hasimah Alimon

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Type :	Article
Subject :	Q Science
ISSN :	1985-7918
Main Author :	Hasimah Alimon
Additional Authors :	W, Paul Quick Richard, C Leegood
Title :	Alteration of fatty acid compositions in a starch-deficient mutant Arabidopsis thaliana (adg1-1) during seed development
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Place of Production :	Tanjong Malim
Publisher :	Fakulti Sains dan Matematik
Year of Publication :	2010
Notes :	Vol. 2 No. 2 (2010): Journal of Science and Mathematics
Corporate Name :	Perpustakaan Tuanku Bainun
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Abstract : Perpustakaan Tuanku Bainun

Disruption of the ADG gene in Arabidopsis results in 6% increase of total fatty acids in mature desiccated seeds. A starchless mutant of Arabidopsis thaliana, ADP glucose pyrophosphorylase (adg1-1) has been investigated concerning the lack of starch in its leaves. In this work of metabolic analysis of adg1-1 mutant, there was very little or no starch accumulation throughout seed development. Even though the growth of these mutant plants was delayed, seed development of the mutants did not show much difference from the wild type. In mature desiccated seeds, there was no significant difference in the seed dry weight of the mutant compared to the wild type. The adg1-1 mutant showed a shift in carbon partitioning to fatty acid (FA) and sucrose accumulation. Major changes have occurred in FA 18.0 (stearic), FA 18.1 (oleic) and FA 18.2 (linoleic). In mature seeds of adg1-1, there was a 70% increase in FA 18.2, a 75% increase in FA 18.1 and a 76% reduction in FA 18.0. The results show that the assimilate partitioning within Arabidopsis seeds has the potential to be altered by relatively simple genetic manipulations. However, the mechanisms by which these changes occurred remain unknown. Keywords starchless mutant, ADP glucose pyrophosphorylase (ADG), carbon partitioning, seed development, metabolism

References

Baud, S., Boutin, J.P., Miquel, M., Lepinee, L., and Rochat, C. (2002). An integrated overview of

seed development in Arabidopsis thaliana ecotype WS. Plant Physiology and Biochemsitry,

40, 151-160.

Bhattacharyya, M., Martin, C. and Smith, A. (1993). The importance of starch biosynthesis in the

wrinkled seed shape character of peas studied by Mendel. Plant Molecular Biology, 22,

525-531.

Bradford, M. (1976). A rapid and sensitive method for the quantification of micrograms quantities

utilizing the principle of protein dye binding. Anal. Biochem, 76, 248-254.

Caspar, T., Huber, S.C. and Somerville, C. (1985). Alterations in growth, photosynthesis, and

respiration in a starchless mutant of Arabidopsis thaliana (L.) deficient in chloroplast

phosphoglucomutase activity. Plant Physiology, 79, 11-17.

Caspar, T., Lin, T.P., Kakefuda, G., Benbow, L., Preiss, J. and Somerville, C. (1991). Mutants of

Arabidopsis with altered regulation of starch degradation. Plant Physiology, 95, 1181-1188.

Casson, A. (1999). The hesitant boom: Indonesia’s oil palm sub-sector in an era of economic crisis

and political change. Bogor, Indonesia: Center for International Forestry Research.

Chung, S. H., Allen, T.W., Hoyles, M., Kuyucak, S. (1999) Permeation of ions across the potassium

channelBrownian dynamics studies. Biophys. J. 75(5): 2517-2533

Dennis, D.T., Turpin, D.H., Lefebvre, D.D. and Layzell, D.B. (1997). Plant metabolism. 2nd Edition.

England: Addison Wesley Longman Ltd.

Edwards, J. and Rees, T. (1985). Sucrose partitioning in developing embryos of round and wrinkled

varieties of Pisum sativum. Phytochemistry, 25(9), 2027-2032.

Focks, N. and Benning, C, (1998). Wrinkled1: a novel, low-seed-oil mutant of Arabidopsis with a

deficiency in the seed-specific regulation of carbohydrate metabolism. Plant Physiology, 118,

91-101.

Jako, C., Kumar, A., Wei, Y., Zou, J., Barton, D.L., Giblin, E.M., Covello, P.S. and Taylor, D.C.

(2001). Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol

acyltransfrease enhances seed oil content and seed weight. Plant Physiology, 126, 861-874.

Johnson, P.E., Patron, N.J., Bottrill, A.R., Dinges, J.R., Fahy, B.F., Parker, M.L., Waite, D.N. and

Denyer, K. (2003). A low-starch barley mutant, risj 16, lacking the cytosolic small subunit

of ADP-glucose pyrophosphorylase, reveals the importance of the cytosolic isoform and the

identity of the plastidial small subunit. Plant Physiology, 131, 684-696.

Kang, F. and Rawsthorne, S. (1994). Starch and fatty acid synthesis in plastids from developing

embryos of oilseed rape (Brassica napus L.). The Plant Journal, 6(6), 795-805.

Kawagoe, Y., Kobo, A., Satoh, H., Takaiwa, F. and Nakamura, Y. (2005). Roles of isoamylase and

ADP-glucose pyrophosphorylase in starch granule in rice endosperm. The Plant Journal,

42(2), 164, doi:10.1111/j.1365-313X.2005.02367.x

Lalonde, S., Boles, E., Hellmann, H., Barker, L., Patrick, J.W., Frommer, W.B. and Ward, J.M.

(1999). The dual function of sugar carriers: transport and sugar sensing. The Plant Cell, 11,

707-726.

Lin, T.P., Caspar, T., Somerville, C. and Preiss, J. (1988). Isolation and characterization of a starchless

mutant of Arabidopsis thaliana (L.) Heynh lacking ADPglucose pyrophosphorylase activity.

J Plant Physiology, 86, 1131-1135.

Plaxton,W.C. and Preiss, J. (1987). Purification and properties of nonproteolyticdegraded ADP

glucose pyrophosphorylase from maize dendosperm. J Plant Physiology, 83, 105-112.

Quick, W.P., Schurr, U., Fichtner, K., Schulze, E.D., Rodermel, S.R., Bogorad, L. and Stitt, M.

(1991). The impact of decreased rubisco on photosynthesis, growth, allocation and storage

in tobacco plants which have been transformed with antisense. Rbcs. Plant J., 1 (1), 51–58.

Reiser, R., Probsfield, J.L and Silvers, A. (1985). Plasma lipid and lipoprotein response of human to

beef fat, coconut oil and safflower oil. Am. J Clin Nutri. 42 (2): 1990-1997.

Routaboul, J.M., Benning, C., Bechtold, N., Caboche, M. and Lepiniec, L. (1999). The TAG1 locus of

Arabidopsis encodes for diacylglycerol acyltransferase. Plant physiology and Biochemistry,

37(11), 831-840.

Ruuska, S.A., Girke, T., Benning, C. and Ohlrogge, J.B. (2004). Contrapuntal networks of gene

expression during Arabidopsis seed filling. The Plant Cell, 14, 1191-1206.

Stitt, M., Lilley, R.M., Gerhardt, R. and Heldt, H.W. (1989). Determination of metabolite levels in

specific cells and subcellular compartment of plant cells. Methods in Enzymology, 174, 518-

552.

Tomlinson, K.L., McHugh, S., Labbe, H,, Grainger, J.L., James, L.E., Pomeroy, K.M., Mullin, J.W.,

Miller, S.S., Dennis, D.T. and Miki, B.L.A. (2004). Evidence that the hexose-to-sucrose ratio

does not control the switch to storage product accumulation in oilseeds: analysis of tobacco

seed development and effects of overexpressing apoplastic invertase. Journal of Experimental

Botany, 55(406), 2291-2303.

Wang, S.M., Lue, W.L., Yu, T.S., Long, J.H., Wang, C.N., Eimert, K. and Che, J. (1998)

Characterization of ADG1, an Arabidopsis locus encoding for ADPG pyrophosphorylase

small subunit is required for large subunit stability. The Plant Journal, 13(1), 63-70.

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