Effects of gamma radiation on growth of Sinningia speciosa callus tissue

Norhayati Daud

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Type :	Article
Subject :	Q Science
ISSN :	1985-7918
Main Author :	Norhayati Daud
Additional Authors :	Rosna Mat Taha
Title :	Effects of gamma radiation on growth of Sinningia speciosa callus tissue
Hits :	7

Place of Production :	Tanjong Malim
Publisher :	Fakulti Sains dan Matematik
Year of Publication :	2011
Notes :	Vol. 3 No. 2 (2011): Journal of Science and Mathematics
Corporate Name :	Perpustakaan Tuanku Bainun
PDF Full Text :	Login required to access this item.

Abstract : Perpustakaan Tuanku Bainun

In this study, the in vitro tissue culture of the Sinningia speciosa callus was established at 25_1 _C with light intensity 1000 _mol m-2 sec-1 and 70-80% relative humidity with a 16/8h light/dark photoperiod. Additionally, gamma radiation of 10-60 Gy was applied and the effects of gamma rays on growth of Sinningia speciosa callus tissue were investigated. A 100% callus initiation was observed in petal explants cultured on MS medium supplemented with combinations of 0.5 mg L-1 NAA and 2.0 mg L-1 BAP resulted in 100% callus induction after being cultured for 4 weeks. This medium was considered optimal medium for callus induction because it can produce normal growing healthy callus. Callus growth was based on callus weight and changing textures and colour of callus; determined following exposure of the calli to different doses of gamma rays. Results obtained indicated that callus growth was reduced when the dose of radiation was increased. It was found that increasing doses of gamma radiation (10-60 Gy) has inhibition effect on callus fresh weight on the 4-week established cultures of Sinningia speciosa callus. Gamma rays produced different effect compared to non-irradiated callus controls in morphological aspects of the callus such as textures and colour of callus cultures. Keywords Sinningia speciosa, gamma rays, callus growth.

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