UPSI Digital Repository (UDRep)
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UPSI Digital Repository (UDRep)
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| Abstract : Perpustakaan Tuanku Bainun |
| Papaya is one of the fruit crops cultivated in Malaysia and contributed to more than USD 2 million in gross production value in the year 2022 which also threatened by plant diseases. Recently, Fusarium equiseti has been observed to cause post-harvest papaya disease in Malaysia. To date, there is limited study on the molecular processes related to the papaya defense mechanism during fungal infection. The objectives of the study are to identify the causal agent causing postharvest disease in Papaya fruit and changes in protein abundance involved in the papaya_s defense mechanism. Firstly, a fungal growth pattern was observed after incubating the samples on Potato Dextrose Agar (PDA), giving evidence of Fusarium involvement in the postharvest disease. F. equiseti on PDA forms fast-growing, woolly colonies with salmon to reddish-brown coloration. After successful amplification using PCR, producing distinct bands corresponding to approximately 583 bp and 576 bp for the first and second isolates, respectively. A BLAST analysis revealed a 100% sequence similarity between the first isolate F. equiseti, with GenBank accession no MN335223.1. Following the establishment of F. equiseti as the pathogenic agent, the study proceeded to investigate the proteomic responses of papaya fruits to Fusarium inoculation. A total of five protein spots were identified, including Oxysterol-binding protein-related protein 4B, Stroma 70kDa heat shock-related protein, Lipoxygenase,30S ribosomal protein, Linoleate 9Slipoxygenase (spots 1324, 1002, 1923, 2138, 2701) respectively. Three proteins were down regulated (spots 1324, 1002, 2138) in response to F. equiseti inoculation, indicating significant in the plant_s defense response. After protein identification using MALDI-TOF/TOF mass spectrometry, total of five protein spots were successfully identified, comprising these proteins with known functions with their differential expression levels in response to F. equiseti inoculation. Throughout string analysis, the heat map of proteins and Gene cooccurrence was used. Gene cooccurrence results showed that the proteins identified from papaya show similarity to Arabidopsis thaliana._ |
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