In situ synthesis and characterisation of chitosan/nanohydroxyapatite composite (IR)

Ahmad Azraie Mat Zulkipli

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Type :	thesis
Subject :	QD Chemistry
Main Author :	Ahmad Azraie Mat Zulkipli
Title :	In situ synthesis and characterisation of chitosan/nanohydroxyapatite composite (IR)

Publisher :	Fakulti Sains dan Matematik
Year of Publication :	2015
Notes :	masters
Corporate Name :	Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris

This study aimed to synthesize and characterise the homogenous chitosan/hydroxyapatite (CS/HA) composite by in situ precipitation method. The optimum ratio of chitosan/hydroxyapatite composite was studied by varying the concentration of chitosan, orthophosphoric acid (H3PO4) and calcium nitrate (Ca(NO3)2.4H2O) used in this formulation. The CS-H3PO4 solution was prepared and reacted with Ca(NO3)2.4H2O to produce CS/HA composites ratio of 30/70, 40/60 and 45/55, which were identified by thermogravimetric analysis. Fourier transform infrared spectroscopy results showed absorption peaks at 601.8 cm-1 and 562.0 cm-1 to 564.2 cm-1 which proved the presence of phosphate, PO43- of HA component whereas absorption peak at 1591.0 cm-1 was corresponding to amide of the chitosan group. Scanning electron microscopy analysis on the composite surface revealed that CS/HA composite with 45/55 ratio exhibited particles with needle like shape with cross-section of 5.41 nm and length of 37.2 nm distributed in the matrix. On the other hand, composite with ratio of 30/70 and 40/60 were more rounded and less uniformly dispersed. The compression strength of 45/55 composite was the highest as compared to 30/70 and 40/60 composite. X-ray powder diffraction result showed similar pattern for all samples, of which chitosan appeared at 2θ = 10.6o and 20.4o while crystalline peaks of HA at 2θ = 25.8o and 40.0o. Bioactivity study proved that mineralization was occurred on the surface of all samples, but it was more significant in 30/70 composite sample. Biocompatibility study revealed that all composites have no indication of cell toxicity. The results showed that the simple in situ precipitation technique successfully developed CS/HA composite with good mechanical properties and biocompatibility, which is suitable for bone filler application.

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