UPSI Digital Repository (UDRep)
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UPSI Digital Repository (UDRep)
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| Abstract : Perpustakaan Tuanku Bainun |
| The aim of this study was to investigate the essential oil composition, phytochemicals, and antibacterial activity of Piper frustratum, Piperaceae. Essential oil was obtained from the leaves of P. frustratum via hydrodistillation and its chemical composition was determined using gas chromatography (GC) and gas chromatography mass spectrometry (GC-MS). Cold extraction was performed to obtain the leaf extract, followed by isolation of phytochemicals using column chromatography techniques, and structural elucidation was achieved via various spectroscopic methods in comparison with literature. Antibacterial activity was evaluated using micro-dilution method. Results showed that nineteen chemical constituents were identified in the essential oil of P. frustratum leaf, accounting for 99.6% of the total composition. The major constituents include _-caryophyllene (12.6%), _-asarone (10.7%), myristicin (10.4%), _-copaene (9.6%), methyl eugenol (7.2 %), and _-cubebene (6.5%). Isolation and purification of the P. frustratum leaf extracts yielded eight phytochemicals including taraxerol, ursolic acid, _-asarone, _-asarone, 3_,4_,5,5_,7-pentamethoxyflavone, asaricin, elemicin, and N-isobutyl-(2E,4E)-tetradecadienamide. In addition, 3_,4_,5,5_,7- pentamethoxyflavone exhibited the strongest antibacterial activity with equal minimum inhibitory concentration (MIC) and minimum bactericidal activity (MBC) values (12.5 _g/mL) against both Escherichia coli and Salmonella typhi. In conclusion, the essential oil of P. frustratum was dominated by sesquiterpene hydrocarbons, while phytochemical analysis of the leaf extracts revealed the presence of terpenes, phenylpropanoids, flavone, and amides. These findings indicate that P. frustratum is a potential natural source for antibacterial compounds for the development of new antimicrobial agents. |
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