Phytochemical study of stem bark from Alstonia spathulata Bl. (Apocynaceae)

Tee Chuan Thing, Lee Yean Shan, Tan Siow Ping, Awang Khalijah, Nafiah Mohd Azlan, Ahmad Kartini,

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Type :	article
Subject :	QD Chemistry
ISSN :	2289-7070
Main Author :	Tee Chuan Thing, Lee Yean Shan, Tan Siow Ping, Awang Khalijah, Nafiah Mohd Azlan, Ahmad Kartini,
Title :	Phytochemical study of stem bark from Alstonia spathulata Bl. (Apocynaceae)

Year of Publication :

2014

Abstract :

In Malaysia, Alstonia spathulata Bl. is locally known as ‘Pulai Basong’ which distribute in peat forest. The separation of the chemical components from hexane and DCM crude extract of Alstonia spathulata was carried out using column chromatography (CC) and thin layer chromatography (TCL) techniques. A total of five known compound were isolated from the stem bark extracts and identified as: β-amyrin, β-amyrin acetate, lupeol acetate, stigmasterol and β-sitosterol. The elucidation structures of these compounds were established using 1D-NMR (1H, 13C and DEPT) and 2D-NMR (COSY, HSQC, HMBC) spectroscopic analysis, involving also comparison with data from the literature.

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