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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| This study aims to examine phycoremediation and biomass production of local microalgae isolates cultivated in high nitrate concentration of nitrified landfill leachate (NLL). The microalgae samples were collected from landfill leachate treatment plant and were isolated as potential species that are able to grow in high nitrate concentration. The isolates were identified using APHA's Key for Identification of Freshwater Algae and 16S rRNAl18S rDNA molecular techniques. Maximum microalgae growth, nitrate removal performance, and biomass productivity were determined at NLL concentration of 10 30% v/v. Biomass produced was further characterized for carbohydrate, lipid, and protein composition. The findings indicated that three species of local microalgae were successfully isolated namely; Chlorella sp., Scenedesmus sp. and Oscillatoria sp. with highest specific growth rate (11) of 0.62 day" (15% NLL), 0.71 day" (10% NLL), and 0.94 day" (10% NLL), respectively. Nitrate was removed by all microalgae isolates with nitrate removal rate of 26.5-27.5 mgl.i'day" and maximum nitrate removal percentage of84% (20% NLL) by Oscillatoria sp. Highest biomass productivity (106.87 mgl.i'day"), carbohydrate productivity (2921.83 mgl.t'day"), lipid productivity (1414.96 mgl.i'day") were observed for Oscillatoria sp. cultured in 10% NLL. Highest protein productivity (5498.37 mgl.i'day") was observed for Oscillatoria sp. cultured in 20% NLL. Highest carbohydrate, lipid, and protein content were observed for Chlorella sp. cultured in 30% NLL (37.50%), Chlorella sp. cultured in 10% NLL (18.23%), and Oscillatoria sp. cultured in 20% NLL (61.4%), respectively. The highest carbohydrate, lipid, and protein yield were achieved by Oscillatoria sp. cultured in 10% NLL (0.95 mg carbohydrate mg" NOJ-), Chlorella sp. cultured in 10% NLL (0.5 mg lipid mg' N03"), and Oscillatoria sp. cultured in 10% NLL (1.58 mg protein rng' N031 respectively. In conclusion, application of local microalgae isolates in phycoremediation of NLL and biomass production was feasible. As implication, local microalgae isolates performance in this research could serves as a basis for scaling up of microalgae biomass production. |
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