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Type :article
Subject :GV Recreation Leisure
ISSN :1112-9867
Main Author :Noor Aiwa Rosman
Additional Authors :Froemming, G.R.A.
Omar Effat
Hapizah Mohd Nawawi
Singh, Harbindarjeet
Title :Effects of adiponectin on markers of endothelial activation and markers of inflammation in human coronary artery endothelial cells
Year of Publication :2017

Abstract :
This study investigates the effect of adiponectin on endothelial activation and inflammatory marker secretion by human coronary artery endothelial cells (HCEAC) in vitro. Methodology: HCAEC at the seventh passage were divided into two groups and incubated for 24 hours at 37° C and 5% CO2 as follows: Control, and adiponectin-treated (30 µg/ml adiponectin) groups. Supernatants were analysed for ICAM-1, E-selectin, PAI-1 and IL-6 and COX-2 using ELISA. RT-PCR was used to analyse gene expression of ICAM-1, E-selectin, PAI-, IL-6, COX-2, NFkBp50 and NFkBp65. Data were analysed using independent t-test.Results: ICAM-1 and E-selectin level was significantly higher in leptin-adiponectin-treated groups (P

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