Culture medium and varietal selection for establishment of callus culture of Artemisia annua

Chin, Chee Keong

Abstract

Plant tissue culture technology offers the potential of producing medicinally important secondary metabolites such as anti-malarial artemisinin from Artemisia annua. In this study, callus induction of three different varieties of A.annua, namely T1, T2 and Hi varieties was carried out using leaf explants on Murashige & Skoog (MS) and Litvay (LV) media with three different supplementations. MS medium added with 0.5 mg/L BA, 0.5 mg/L NAA and 0.5 g/L of casein hydrolysate (CH) induced the highest yield of callus biomass compared to the effect of picloram- or 2,4-enriched MS medium. T2 variety was found to be the highest yielding variety in this medium. Picloram-enriched MS medium induced better callus in term of callus biomass and friability than 2,4-enriched medium. Hi variety induced in MS medium added with 0.5 mg/L picloram produced highest callus biomass among the three varieties. Callus formed on 0.5 mg/L picloram was also much more easily dispersed than callus of all three varieties cultured in the other two MS medium. LV-based medium was generally shown to be poor in inducing callogenic response from leaf explants. Therefore Hi callus sourced from MS medium supplemented with 0.5 mg/L picloram was selected to initiate liquid cell culture of A. annua which can further be explored for production of artemisinin, an anti-malarial compound.

References

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