Keberkesanan antibiotik titisan mata Gentamicin terhadap Sista Acanthamoeba spp. Isolat Klinikal

Mohamed Kamel Abd Ghani

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Type :	article
Subject :	Q Science (General)
ISSN :	2462-2052
Main Author :	Mohamed Kamel Abd Ghani
Additional Authors :	Noramalina Anisah Nordin Yusof Suboh Noraina Abd Rahim Norazah Ahmad
Title :	Keberkesanan antibiotik titisan mata Gentamicin terhadap Sista Acanthamoeba spp. Isolat Klinikal

Place of Production :	Universiti Pendidikan Sultan Idris
Year of Publication :	2018

Abstract :

Acanthamoeba spp. boleh menyebabkan keratitis kepada manusia, khasnya kepada pemakai kanta sentuh yang tidak menitikberatkan aspek higen kanta sentuh. Kajian ini bertujuan untuk mengkaji keberkesanan antibiotik titisan mata Gentamicin terhadap sista Acanthamoeba isolat klinikal iaitu HKL 5, HKL 11, HKL 32, dan HKL 51. Kajian dimulakan dengan melakukan suspensi sista terhadap keempat-empat isolat klinikal tersebut. Mikropencairan terhadap antimikrob dilakukan dan kemudiannya suspensi sista ditambah, seterusnya dieramkan selama 24 jam pada 30ºC sebelum dipindahkan ke atas plat agar bukan nutrien (NNA) yang dilapisi E. coli matian haba. Plat agar diperhatikan setiap hari sehingga hari yang ke-14 untuk mengesan sebarang kehadiran trofozoit di bawah mikroskop inverted. Kehadiran trofozoit Acanthamoeba menunjukkan bahawa antibiotik ujian tidak berkesan terhadap sista Acanthamoeba. Gentamicin berkesan terhadap sista Acanthamoeba, dengan nilai purata sistisidal minimum (MCC) 1453.1 μg/ml. Gentamicin merupakan antibiotik yang boleh digunakan dalam rawatan keratitis Acanthamoeba memandangkan kepada keberkesanannya terhadap sista Acanthamoeba isolat klinikal dalam kajian ini.

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